JOURNAL OF SHANDONG UNIVERSITY (OTOLARYNGOLOGY AND OPHTHALMOLOGY) ›› 2017, Vol. 31 ›› Issue (4): 60-63.doi: 10.6040/j.issn.1673-3770.0.2016.362

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Expression and methylation patterns of CDH13 in human head and neck squamous carcinoma cells.

ZHAO Jincheng, SHI Ying, ZHANG Ying, JIA Zhanhong, MA Xin, ZHANG Jingqiu, WU Zaijun, WANG Yu   

  1. Department of Otolaryngology-Head and Neck Surgery, 263 Clinical Department of General Army Hospital, Beijing 101149, China
  • Received:2016-08-17 Online:2017-08-16 Published:2017-08-16

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Abstract: Objective To explore the expression levels and methylation patterns of the tumor-suppressor gene H-Cadherin 13(CDH13)in the head and neck squamous cell carcinoma(HNSCC)SCC10A and PCI-37B cell lines. Methods We selected 2 normal oral mucosa tissues as the control group, and used quantitative PCR and western blotting, respectively, to detect the relative mRNA and protein expression levels of CDH13 in SCC10A and PCI-37B cells. We then analyzed the methylation status of the promoter region of CDH13 using methylation-specific PCR(MSP)and bisulfite sequencing PCR(BSP). Results The mRNA and protein expression levels of CDH13 in SCC10A and PCI-37B were significantly reduced compared to the control group. Methylation of the CDH13 promoter was considerably higher in SCC10A and PCI-37B cells than in normal tissues; BSP confirmed this finding. Conclusion The high methylation levels of the CDH13 gene promoter may play an important role in down-regulating the expression of CDH13 in HNSCC cells, but further study is required to determine its influence on HNSCC.

Key words: Head and neck squamous cell carcinoma, Expression, Methylation, CDH13

CLC Number: 

  • R739.91
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