J Otolaryngol Ophthalmol Shandong Univ ›› 2018, Vol. 32 ›› Issue (4): 43-47.doi: 10.6040/j.issn.1673-3770.0.2017.433

Previous Articles     Next Articles

Establishment of a standardized model of traumatic optic neuropathy in cats

LI Junyi1, LI Xiumei2, WAN Jingming1, BI Jingyu1, LIU Juan1, XU Hualin1, JIANG Yan3, LI Na3   

  1. Shanxian Hospital of Traditional Chinese Medicine, Shanxian 274300, Shandong, China;3. Department of Otolaryngology Head and Neck Surgery, The Affiliated Hospital of Qingdao University Medical College, Qingdao 266003, Shandong, China
  • Received:2017-10-10 Online:2018-07-20 Published:2018-07-20

PDF (PC)

527

Abstract: Objective To establish a standardized model of traumatic optic neuropathy in cats by using a standard clip and to simultaneously observe the changes in flash visual-evoked potential. Methods Ten adult healthy cats were enrolled in the study. The right eye was identified as the experimental eye; and the left eye, as the control eye. The optic nerves of the right eyes were clamped for 30 seconds to establish traumatic optic nerve injury models. The flash visual-evoked potential in was recorded in all experimental and control eyes at 1 hour, 3 days, 1 week, 2 weeks, and 4 weeks after injury. The peak latency and amplitude of the main wave P100 were recorded, and a statistical analysis was performed. Results The flash visual evoked potential peak latency and amplitudes of the main wave P100 were no significant differences before operation, but there were significant differences after traumatic optic neuropathy between the two groups(P< 0.05). The flash visual evoked potential peak latency and amplitudes of the main wave P100 were no significant differences before operation(F=1.48, P=0.19; F=1.63, P=0.15). In the experimental group, there were statistically significant differences between the 1 hour after injury and 3 days, 1 week, 2 weeks, and 4 weeks after the injury(F=7.38, P=0.008 7; F=4.38, P=0.027). There were statistically significant differences between 3 days after the injury and 1 week,2 weeks and 4 weeks after the injury(F=6.49, P =0.019; F=3.97, P=0.036). There were statistically significant differences between 1 week after the injury and 2 weeks and 4 weeks after the injury(F=6.02, P=0.011; F=3.37, P=0.041).The peak latency of P100 was gradually extended, and the amplitude gradually decreased in the 2 weeks after the injury. After 2 weeks, it tended to be stable. There was no statistically significant difference between the two groups after 2 weeks and 4 weeks after the injury(F=2.24, P=0.11; F=2.01, P=0.12). Conclusion The standardized cat model of traumatic optic neuropathy was successfully established by using the same clamp. The flash visual-evoked potential examination is the objective evaluation tool. The flash visual-evoked potential peak latency of the main wave P100 was significantly prolonged with a decreased amplitude, in the standardized cat model of traumatic optic neuropathy.

Key words: Optic nerve injury, Flash visual evoked potential, Clamp, Model

CLC Number: 

  • R774.6
[1] 姜彦,李娜,张念凯,等.家猫外伤性视神经病的电生理及形态学观察[J].中华耳鼻咽喉头颈外科杂志,2007,42(7):520-525. JIANG Yan, LI Na, ZHANG Niankai, et al. Observation of neural electrophysiology and morphology in traumatic optic neuropathy in cat model[J]. Chin J Otorhinolaryngol Head Neck Surgery, 2007, 42(7):520-525.
[2] 丁见,杨华,张念凯.猫视神经损伤减压手术模型的建立[J].山东大学耳鼻喉眼学报,2009,23(3):75-77. DING Jian, YANG Hua, ZHANG Niankai. Model establishment of optic nerve decompression surgery in cats[J]. J Otolaryngol Ophthalmol Shandong University, 2009, 23(3):75-77.
[3] 赵玲,冯秀艳.视觉诱发电位在外伤性视神经病治疗中的临床应用[J].中华眼外伤职业眼病杂志,2012,34(1):51-53. ZHAO Ling, FENG Xiuyan. VEP clinical application in the treatment of traumatic optic neuropathy[J]. Chin J Ocular Trauma Occupational Eye Dis, 2012, 34(1):51-53.
[4] 周鑫.眼外伤后视力的视觉诱发电位评估[J].国际眼科杂志,2013,13(10):2068-2070. ZHOU Xin. Evaluation of visual evoked potentials in post-trauma eyes[J]. Inter Eye Sci, 2013, 13(10):2068-2070.
[5] Tao W, Dvoriantchikova G, Tse BC, et al. A novel mouse model of traumatic optic neuropathy using external ultrasound energy to achieve focal, indirect optic nerve injury[J]. Sci Reports, 2017, 7(1):11779.
[6] 曹霞,姜晓璇,唐永赢,等.大鼠视神经钳夹伤模型的建立及磁共振成像运用初探[J].眼科新进展,2014,34(3):205-208. CAO Xia, JIANG Xiaoxuan, TANG Yongying, et al. Establishment and pre-application of magnetic resonance imaging in rat optic nerve clamping injury model[J]. Rec Adv Ophthalmol, 2014, 34(3):205-208.
[7] 陈二涛,刘勇,冯东福,等.大鼠视神经部分损伤模型的建立[J].中华实验外科杂志,2008,25(11):1514-1516. CHEN Ertao, LIU Yong, FENG Dongfu, et al. Development of experimental partial optic nerve injury model in rats[J]. Chin J Exp Surg, 2008, 25(11):1514-1516.
[8] 李林林,温晏,宫华青.图形视觉诱发电位在猫双眼视觉研究中的作用[J].国际眼科杂志,2013,13(5):865-868. LI Linlin, WEN Yan, GONG Huaqing. The study of P-VEP on binocular vision in cats[J]. Inter Eye Sci, 2013, 13(5):865-868.
[9] 韩晓霞,许文辉,羊正祥,等.视神经损伤经颅视神经管减压与视觉诱发电位的临床研究[J].临床神经外科杂志,2014,11(6):459-461. HAN Xiaoxia, XU Wenhui, YANG Zhengxiang, et al. Clinical study on transcranial optic canal decompression and visual evoked potential for optic nerve injury[J]. J Clin Neurosurg, 2014, 11(6):459-461.
[10] 贾云峰,胡国良,李建.颅脑损伤伴视神经损伤86例临床分析[J].临床神经外科杂志,2013,10(2):90-91. JIA Yunfeng, HU Guoliang, LI Jian. Clinical analysis of 86 patients with head trauma and traumatic optic neuropathy[J]. J Clin Neurosurg, 2013, 10(2):90-91.
[11] 魏洪润,张哲成,田丽,等.视觉诱发电位对糖尿病视网膜病变的早期诊断价值[J].山东医药,2014,54(13):45-46.
[12] Luo Yi-ting. Diagnostic value of VEP applied in patients with optic nerve contusion[J].Mod Diagn Treat, 2013, 24(5):963-964.
[13] 赵秋岭.视觉诱发电位对外伤性视神经损伤诊断的应用价值[J].中国伤残医学,2014,22(9):206-207.
[14] You Y, K1istomar A, Thie J, et al. Axonal loss in a rat model of optic neuritisis closely correlated with visual evoked potential amplitudes using electroencephalogram-based sealing[J]. Invest Ophthalmol Vis Sci, 2012, 53(7):3662-3663.
[15] Heravian J, Ehyaei A, Shoeihi V, et al. Pattern Visual Evoked potentials patients with type II diabetes mellitus[J]. Ophthamic Vis Res, 2012, 7(3):225-230.
[16] Xue F, Wu K, Wang T, et al. Morphological and functional changes of the optic nerve following traumatic optic nerve injuries in rabbits[J]. Biomed Rep, 2016, 4(2):188-192.
[1] LI Kai, LUO Dan. Runmu ling formula ameliorates ocular surface damage in dry eye rats by activating the LC3-ATG5 autophagy pathway and inhibiting inflammatory factor expression [J]. Journal of Otolaryngology and Ophthalmology of Shandong University, 2026, 40(3): 92-101.
[2] LIU Jiahui, TIAN Ruxian, LI Yumei, SONG Xicheng. Construct of a cuproptosis-related lncRNA model to predict prognosis in head and neck squamous cell carcinoma [J]. Journal of Otolaryngology and Ophthalmology of Shandong University, 2026, 40(2): 49-64.
[3] ZHANG Nana, HUANG Guanjiang, LU Biaoqing. Analysis of influencing factors for laryngeal carcinogenesis and construction of a predictive model [J]. Journal of Otolaryngology and Ophthalmology of Shandong University, 2026, 40(1): 29-37.
[4] QIN Nana, LI Yufen, SUN Yuhao, WEI Jian, LI Qin. Effect of interleukin-13 receptor-α2 on nasal mucosal remodeling in rats with allergic rhinitis by TGF-β1/Smad signaling pathway [J]. Journal of Otolaryngology and Ophthalmology of Shandong University, 2025, 39(6): 71-77.
[5] LIU Danfeng, PAN Qingchun, LI Daibo, FENG Xuefei, REN Jian. Burden of larynx cancer attributable to excessive alcohol consumption and prediction of future trends in China from 1990 to 2021 [J]. Journal of Otolaryngology and Ophthalmology of Shandong University, 2025, 39(5): 61-69.
[6] YAO Xue, LU Xiaofeng, ZHANG Mengrui, HU Xinya, ZHAO Jialuo, LAI Sisi, LI Xuan, LIU Zixiao, SHEN Chaofan, FAN Zixin, ZHANG Yinsheng, ZHANG Guoming. Research on auxiliary diagnosis of ocular oblique muscle dysfunction based on the YOLOv8 model [J]. Journal of Otolaryngology and Ophthalmology of Shandong University, 2025, 39(5): 76-82.
[7] LYU Yong, FENG Yun. Prediction model of cardiovascular disease risk in people with dual sensory impairment: analysis based on CHARLS [J]. Journal of Otolaryngology and Ophthalmology of Shandong University, 2025, 39(3): 122-128.
[8] LI Xiaoyu, WANG Binchen, XU Dapeng, WANG Yanhua, DU Pinggong, ZHANG Qingquan. Effectiveness of transnasal and transoral fenestration in the treatment of maxillary bone cysts [J]. Journal of Otolaryngology and Ophthalmology of Shandong University, 2025, 39(2): 72-78.
[9] LI Zhicheng, YAN Ya, DAI Liangping, YING Junjie, WANG Renzhong. Research progress of animal models of olfactory disorders [J]. Journal of Otolaryngology and Ophthalmology of Shandong University, 2025, 39(2): 158-167.
[10] WANG Wenqing, ZHANG Dan, ZHU Mengdi, WANG Luyang, YANG Peipei, SUN Sisi, ZHANG Qiumin, ZHOU Hui. Changes of clinical and histopathological characteristics in recurrent chronic rhinosinusitis with nasal polyps [J]. Journal of Otolaryngology and Ophthalmology of Shandong University, 2025, 39(1): 46-53.
[11] BI Zhaojing, LI Yuanbin. Current status of experimental models and applications of meibomian gland dysfunction [J]. Journal of Otolaryngology and Ophthalmology of Shandong University, 2024, 38(4): 159-165.
[12] WANG Jingsong, YU Can, ZHANG Xi, DENG Qicheng, XIE Zhuoliang, ZHAO Rui, WEN Bei, LIU Hai. Expression and clinical significance of KRT4 in laryngeal carcinoma and adjacent normal mucosal tissues [J]. Journal of Otolaryngology and Ophthalmology of Shandong University, 2024, 38(3): 12-17.
[13] ZHANG Shihan, LIU Hongbing. Effect of matrix metalloproteinase on tissue remodeling in chronic rhinosinusitis [J]. Journal of Otolaryngology and Ophthalmology of Shandong University, 2024, 38(3): 116-123.
[14] ZHANG Yongcai, ZHANG Jiang, LU Hui, XING Kexin, WU Jingfang, XUE Gang, LI Yanping, LIU Yanbin. Expression and significance of CXCL10 and IFN-γ in antrochoanal polyp [J]. Journal of Otolaryngology and Ophthalmology of Shandong University, 2024, 38(2): 7-12.
[15] DAI Honglei, WANG Qiuyang, MA Wenxue, GUAN Bin, QI Jingjing. To analyze the mortality trend of nasopharyngeal carcinoma based on Joinpoint regression and an age-period-cohort model [J]. Journal of Otolaryngology and Ophthalmology of Shandong University, 2024, 38(1): 27-31.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
No Suggested Reading articles found!
Website Copyright: Editorial Office of Journal of Otolaryngology and Ophthalmology of Shandong University
Address: No.27 Shanda South Road, Jinan, Shandong, China. Post code: 250100 Tel: +86-0531-88366912 Email: ebhxb@sdu.edu.cn Supported by Beijing Magtech Co.Ltd