Journal of Otolaryngology and Ophthalmology of Shandong University ›› 2019, Vol. 33 ›› Issue (5): 69-72.doi: 10.6040/j.issn.1673-3770.0.2018.425

• Original Article • Previous Articles     Next Articles

Expression and biological function of long chain non-coding cytoskeleton regulator RNA in nasopharyngeal carcinoma

Lin LI1,Heng ZHANG2,Yufei XIAN3,Xiaoli SHENG3()   

  1. 1. Department of Otorhinolaryngology Head and Neck Surgery, Zhuhai Hospital of Guangdong Provincial People's Hospital，Jinwan Central Hospital, Zhuhai 519040, Guangdong, China
    2. Department of Pathology, the First Affiliated Hospital of Guangzhou Medical University, Guangzhou 510080, Guangdong, China
    3. Department of Otorhinolaryngology Head and Neck Surgery, Guangdong Academy of Medical Sciences, Guangdong Province People's Hospital, Guangzhou 510080, Guangdong, China
  • Received:2018-09-26 Revised:2018-11-08 Online:2019-09-20 Published:2019-10-15
  • Contact: Xiaoli SHENG E-mail:shengzhang2016@126.com

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Abstract: Objective

To investigate the expression and biological function of long non-coding cytoskeleton regulator RNA (CYTOR) in nasopharyngeal carcinoma.

Methods

Real-time PCR was used to examine CYTOR expression in nasopharyngeal carcinoma tissues (n = 32), nasopharyngeal carcinoma cell lines (CNE1 and HONE1), 28 patients with benign nasopharyngeal lesions, and human nasopharyngeal epithelial cells (NP69). Moreover, CYTOR expression in the CNE1 and HONE1 cell lines was knocked down using RNAi assay. MTT, Transwell, and wound healing assays were also used to detect the proliferation and migration of nasopharyngeal carcinoma cells from CNE1 and HONE1 after downregulating CYTOR expression.

 Results

CYTOR expression in the nasopharyngeal carcinoma tissues and nasopharyngeal carcinoma cell lines was observed to be significantly higher than that in the nasopharyngeal benign lesion mucosal tissues and NP69. After transient inhibition of CYTOR expression, the migration and proliferation abilities of the nasopharyngeal carcinoma cell lines were also observed to decrease significantly (P < 0.05).

 Conclusion

CYTOR expression is elevated in pharyngeal cancer tissues and nasopharyngeal carcinoma cell lines and is positively correlated with the migration ability of nasopharyngeal carcinoma.

Key words: cytoskeleton regulator RNA, Nasopharyngeal Carcinoma, Invasion, Metastasis

CLC Number: 

  • R739.6

Table 1

Downregulation of CYTOR expression inhibits nasopharyngeal carcinoma cell migration"

时间 组别 宽度 F P
0 h 0.22 0.81
CNE1 406.32±22.11
CNE1_SiNC 411.36±19.21
CNE1_SiCYTOR 414.21±26.06
24 h 46.69 <0.001
CNE1 102.11±26.21
CNE1_SiNC 116.65±30.14
CNE1_SiCYTOR 336.45±45.69

Fig. 1

Effect of the inhibition of CYTOR expression on nasopharyngeal carcinoma cell invasion"

1 Wahlestedt C . Targeting long non-coding RNA to therapeutically upregulate gene expression[J]. Nat Rev Drug Discov, 2013, 12(6): 433-446. doi:10.1038/nrd4018 .
doi: 10.1038/nrd4018
2 Zhang H , Chen ZH , Wang XX , et al . Long non-coding RNA: a new player in cancer[J]. J Hematol Oncol, 2013, 6: 37. doi:10.1186/1756-8722-6-37 .
doi: 10.1186/1756-8722-6-37
3 Guttman M , Rinn JL . Modular regulatory principles of large non-coding RNAs[J]. Nature, 2012, 482(7385): 339-346. doi:10.1038/nature10887 .
doi: 10.1038/nature10887
4 Wierzbicki AT . The role of long non-coding RNA in transcriptional gene silencing[J]. Curr Opin Plant Biol, 2012, 15(5): 517-522. doi:10.1016/j.pbi.2012.08.008 .
doi: 10.1016/j.pbi.2012.08.008
5 Liang JY , Wei X , Liu ZH , et al . Long noncoding RNA CYTOR in cancer: a TCGA data review[J]. Clin Chim Acta, 2018, 483: 227-233. doi:10.1016/j.cca. 2018. 05. 010 .
doi: 10.1016/j.cca. 2018. 05. 010
6 Yue B , Liu CC , Sun HM , et al . A positive feed-forward loop between LncRNA-CYTOR and wnt/β-catenin signaling promotes metastasis of colon cancer[J]. Mol Ther, 2018, 26(5): 1287-1298. doi:10.1016/j.ymthe.2018. 02. 024 .
doi: 10.1016/j.ymthe.2018. 02. 024
7 Liongue C , Taznin T , Ward AC . Signaling via the CytoR/JAK/STAT/SOCS pathway: Emergence during evolution[J]. Mol Immunol, 2016, 71: 166-175. doi:10.1016/j. molimm.2016.02.002 .
doi: 10.1016/j. molimm.2016.02.002
8 Wu JL , Shuang ZY , Zhao JF , et al . Linc00152 promotes tumorigenesis by regulating DNMTs in triple-negative breast cancer[J]. Biomed Pharmacother, 2018, 97: 1275-1281. doi:10.1016/j.biopha.2017.11.055 .
doi: 10.1016/j.biopha.2017.11.055
9 Kamran SC , Riaz N , Lee N . Nasopharyngeal carcinoma[J]. Surg Oncol Clin N Am, 2015, 24(3): 547-561. doi:10.1016/j.soc.2015.03.008 .
doi: 10.1016/j.soc.2015.03.008
10 陆海军, 刘霁, 丁晓 . 鼻咽癌的综合治疗研究进展[J]. 山东大学耳鼻喉眼学报, 2019, 33(2): 26-30. doi: 10.6040/j.issn.1673-3770.1.2019.010 .
doi: 10.6040/j.issn.1673-3770.1.2019.010
LU Haijun , LIU Ji , DING Xiao . Progress in the comprehensive treatment for nasopharyngeal carcinoma[J]. Journal of Otolaryngology and Ophthalmology of Shandong University, 2019, 33(2): 26-30. doi: 10.6040/j.issn.1673-3770.1.2019.010 .
doi: 10.6040/j.issn.1673-3770.1.2019.010
11 陈瑞, 李杰恩 . 鼻咽癌放疗后诱发头颈部恶性肿瘤的治疗[J]. 山东大学耳鼻喉眼学报, 2019, 33(2): 31-34. doi:10.6040/j.issn.1673-3770.0.2019.072 .
doi: 10.6040/j.issn. 1673-3770.0.2019.072
CHEN Rui , LI Jie′en . Treatment of radiation-induced carcinoma in patients with nasopharyngeal carcinoma[J]. Journal of Otolaryngology and Ophthalmology of Shandong University, 2019, 33(2): 31-34. doi:10.6040/j.issn. 1673-3770.0.2019.072 .
doi: 10.6040/j.issn. 1673-3770.0.2019.072
12 Deniz E , Erman BT . Long noncoding RNA (lincRNA), a new paradigm in gene expression control[J]. Funct Integr Genomics, 2017, 17(2/3): 135-143. doi:10. 1007/s10142-016-0524-x .
doi: 10. 1007/s10142-016-0524-x
13 Sheng L , Ye L , Zhang D , et al . New insights into the long non-coding RNA SRA: physiological functions and mechanisms of action[J]. Front Med (Lausanne), 2018, 5: 244. doi:10.3389/fmed.2018.00244 .
doi: 10.3389/fmed.2018.00244
14 Zampetaki A , Albrecht A , Steinhofel K . Long non-coding RNA structure and function: is there a link?[J]. Front Physiol, 2018, 9: 1201. doi:10.3389/fphys. 2018. 01201 .
doi: 10.3389/fphys. 2018. 01201
15 Bian ZH , Zhang JW , Li M , et al . Correction: Long non-coding RNA LINC00152 promotes cell proliferation, metastasis, and confers 5-FU resistance in colorectal cancer by inhibiting miR-139-5p[J]. Oncogenesis, 2018, 7(8): 63. doi:10.1038/s41389-018-0067-1 .
doi: 10.1038/s41389-018-0067-1
16 Chen PX , Fang XL , Xia B , et al . Long noncoding RNA LINC00152 promotes cell proliferation through competitively binding endogenous miR-125b with MCL-1 by regulating mitochondrial apoptosis pathways in ovarian cancer[J]. Cancer Med, 2018, 7(9): 4530-4541. doi:10.1002/cam4.1547 .
doi: 10.1002/cam4.1547
17 Hu XL , Wang J , He W , et al . Down-regulation of lncRNA Linc00152 suppressed cell viability, invasion, migration, and epithelial to mesenchymal transition, and reversed chemo-resistance in breast cancer cells[J]. Eur Rev Med Pharmacol Sci, 2018, 22(10): 3074-3084. doi:10.26355/eurrev_201805_15067 .
doi: 10.26355/eurrev_201805_15067
18 Cai Q , Wang ZQ , Wang SH , et al . Long non-coding RNA LINC00152 promotes gallbladder cancer metastasis and epithelial-mesenchymal transition by regulating HIF-1α via miR-138[J]. Open Biol, 2017, 7(1): 160247. doi:10.1098/rsob.160247 .
doi: 10.1098/rsob.160247
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