山东大学耳鼻喉眼学报 ›› 2013, Vol. 27 ›› Issue (1): 19-23.doi: 10.6040/j.issn.1673-3770.0.2012.131

• 论著 • 上一篇    下一篇

大鼠面神经压榨对面神经元CNTF表达的影响

刘悦1,4,李培华2,刘稳2,神平2,许学谷2,孙光明2,孙海清3   

  1. 1.徐州医学院研究生学院, 江苏 徐州 221002;  2.徐州医学院附属医院耳鼻咽喉科, 江苏 徐州 221002;  3.江苏
    曼迪科技发展有限公司, 江苏 徐州 221000; 4.常州市第二人民医院耳鼻咽喉科, 江苏 常州 213003
  • 收稿日期:2012-04-15 发布日期:2013-02-16
  • 通讯作者: 刘稳,博士,副主任医师。 E-mail:liuwen1972@163.com
  • 作者简介:刘悦,硕士研究生,现在常州市第二人民医院工作。 E-mail: liuyue139148@163.com
  • 基金资助:

    江苏省2010年度“企业博士集聚计划”资助项目

Effects of crush injury to the facial nerve on the expressions of CNTF in motoneurons of facial nucleus in rats

LIU Yue1,4, LI Pei-hua2, LIU Wen2, SHEN Ping2, XU Xue-gu2, SUN Guang-ming2, SUN Hai-qing3   

  1. 1. Graduate School, Xuzhou Medical College, Xuzhou 221002, Jiangsu, China;   2. Department of Otolaryngology, Affiliated Hospital of Xuzhou Medical College, Xuzhou 221002, Jiangsu, China;  3. Mandy Jiangsu Science and Technology Development Co. Ltd. Xuzhou 221000, Jiangsu,  China; 4. Department of Otolaryngology, the Second People’s Hospital of Changzhou, Changzhou 213003, Jiangsu, China
  • Received:2012-04-15 Published:2013-02-16

摘要:

目的   观察将大鼠面神经压榨后面部行为学变化,探索简易稳定的面神经压榨建模方法;检测压榨侧面神经运动神经元中睫状神经营养因子(CNTF)的表达变化。方法   选取健康大鼠30只,解剖右侧面神经主干,蚊式血管钳单齿钳夹,持续1min,设左侧为对照。术后1 、7、14 、21、35d,观察大鼠面部行为学改变;HE染色观察面神经运动神经元的病理形态学变化并进行细胞计数;免疫组化观察面神经元CNTF蛋白的表达并进行光密度分析。结果   面神经压榨损伤后即刻出现同侧面神经瘫痪,术后7~14d达高峰,而后逐渐恢复,35d大鼠面部对称,面瘫完全恢复。HE染色发现术后7、14、21d损伤侧面神经运动神经元数目较对照组减少 (P<0.01),细胞结构形态紊乱,细胞内空泡增多,核仁偏移。免疫组化发现术后7、14、21d面神经运动神经元CNTF蛋白表达量明显增多(P<0.01)。结论   神经压榨是建立可逆性面神经麻痹动物模型简易稳定的方法。面神经压榨损伤后,面神经运动神经元内CNTF蛋白的表达增多。

关键词: 面神经核, 压榨损伤, 面神经, 睫状神经营养因子

Abstract:

Objective   To observe the behavioral difference after the crush injury of facial nerve in rats and determine the expression of CNTF in motoneurons of facial nucleus. Methods   In 30 healthy adult rats, the trunk of right facial nerve were crushed while that of the left nerve was control. The rats were divided into 5 subgroups according to the days after the operation, including 1d, 7d, 14d, 21d and 35d subgroup. During the experiment, the behavioral changes were monitored, while the pathological differences of motoneurons were observed by HE staining and the number of cells was counted. CNTF protein in facial nucleus were detected by immunohistochemical method and analyzed by optical density. Results   The rats showed right facial palsy immediately after the crush injury. The number of neurons in the right side decreased significantly compared with the left side (P<0.01) on the 7th, 14th and 21st day. Some morphological changes were observed, such as deranged cellular structure, cytoplasm vacuolization, or nucleolar margination. The expressions of CNTF increased obviously in the right side on the day of  7th, 14th and 21st day (P<0.01). Conslusion   Nerve crush is a simple, easy and stable way to establish animal model for the reversible facial paralysis. The expression of CNTF protein increases in the motoneurons of facial nucleus after the facial nerve is crushed.

Key words: Facial nerve, Crush injury, Ciliary neurotrophic factor, Facial nucleus

中图分类号: 

  • R745.1
[1] 陈涛,刘善平,卢永田. 中耳炎鼓室窦CT分型与手术方式初探[J]. 山东大学耳鼻喉眼学报, 2018, 32(4): 14-16.
[2] 谢邵兵,张燕妮,徐震航,张建辉,吴学文,孙虹. 外伤性面神经麻痹手术时机的系统评价[J]. 山东大学耳鼻喉眼学报, 2016, 30(6): 18-21.
[3] 王一红, 李瑞玉, 陈彩龙, 王晓燕, 林美福, 周硕. MSCT多模态三维重建观测颞骨结构及对人工耳蜗手术的指导意义[J]. 山东大学耳鼻喉眼学报, 2015, 29(6): 1-4.
[4] 王晓侠, 姚小宝, 李宏慧, 白艳霞, 张少强, 申震, 李白芽. 腮腺手术中寻找面神经技巧初探[J]. 山东大学耳鼻喉眼学报, 2015, 29(4): 48-50.
[5] 苟栋明1,王海1,陈建超2. 近发际缘内头皮冠状切口径路在耳鼻咽喉头颈外科手术中应用的临床意义[J]. 山东大学耳鼻喉眼学报, 2014, 28(1): 62-64.
[6] 单良1,陈文文1,倪关森1,罗禹2. 周围性面瘫的MRI增强扫描检查和损伤节段观察及预后相关性分析[J]. 山东大学耳鼻喉眼学报, 2013, 27(6): 28-30.
[7] 刘骏1,赵宇2,邹剑2,秦学玲2. 经乳突进路面神经减压术治疗中耳胆脂瘤并发面神经损害75例[J]. 山东大学耳鼻喉眼学报, 2013, 27(1): 31-32.
[8] 全世明1,彭本刚1,高志强2. T细胞免疫缺陷小鼠创伤性面瘫模型的建立及其形态学评价[J]. 山东大学耳鼻喉眼学报, 2010, 24(6): 5-9.
[9] 秦晨,王海波,李建峰,韩月臣,刘闻闻,逯素梅. 大鼠面神经损伤后神经营养因子-4的动态表达[J]. 山东大学耳鼻喉眼学报, 2010, 24(2): 17-.
[10] 崔鹏,王海波,李建峰,韩月臣,张海燕,徐磊
. 面神经损伤后糖皮质激素受体α在面神经核及
外周淋巴组织的表达
[J]. 山东大学耳鼻喉眼学报, 2009, 23(2): 34-37 .
[11] 刘 艳,刘新义,王金平,李大健 . 后鼓室解剖结构测量观察及临床意义[J]. 山东大学耳鼻喉眼学报, 2008, 22(3): 218-221 .
[12] 张丽丽 综述,王海波 审校 . 神经逆行示踪法在面神经损伤修复方面的应用[J]. 山东大学耳鼻喉眼学报, 2008, 22(2): 182-184 .
[13] 罗建芬,樊兆民,王海波 . 颞骨骨折性面瘫手术减压时机的实验研究[J]. 山东大学耳鼻喉眼学报, 2008, 22(1): 85-87 .
[14] 李新民,张庆泉,宋西成,王锡温,张天振,王强 . 手术治疗面神经鞘瘤[J]. 山东大学耳鼻喉眼学报, 2006, 20(6): 549-551 .
[15] 全世明,高志强 . 面神经修复及再生的组织工程学研究进展[J]. 山东大学耳鼻喉眼学报, 2006, 20(2): 97-101 .
Viewed
Full text


Abstract

Cited

  Shared   
  Discussed   
No Suggested Reading articles found!