18β-甘草次酸对变应性鼻炎大鼠鼻黏膜上皮细胞线粒体的影响

doi:10.6040/j.issn.1673-3770.0.2014.062

山东大学耳鼻喉眼学报

• 应变性鼻炎 • 下一篇

18β-甘草次酸对变应性鼻炎大鼠鼻黏膜上皮细胞线粒体的影响

董明1，陈小婉1,2，席克虎1,2，王有虎1,2，桂岩1,2，张福宏1,2，马春霞1，洪浩1，刘翔毅2，江英1，马毅1，杨贵军1，张小兵1,2

1.兰州大学第一医院耳鼻咽喉头颈外科，甘肃兰州 730000;
2.甘肃中医学院附属医院耳鼻咽喉头颈科, 甘肃兰州 730020

收稿日期:2014-02-20 出版日期:2014-06-16 发布日期:2014-06-16
通讯作者: 张小兵。 E-mail：790736924@qq.com
作者简介:董明。 E-mail：guyverthree@foxmail.com
基金资助:
国家自然科学基金项目(81160449)；甘肃省卫生厅行业科技计划项目(GSWST2011-06)；甘肃省中医药科学技术研究课题(GZK-2011-22)

Influence of 18-βglycyrrhetinic acid on mitochondria of nasal mucosa epithelial cells in rats with allergic rhinitis

DONG Ming1, CHEN Xiao-wan1,2, XI Ke-hu1,2, WANG You-hu1,2, GUI Yan1,2, ZHANG Fu-hong1,2, MA Chun-xia1, HONG Hao1, LIU Xiang-yi2, JIANG Ying1, MA Yi1, YANG Gui-jun1, ZHANG Xiao-bing1,2.

Department of Otolaryngology, 1. First Hospital of Lanzhou University, Lanzhou 730000, Gansu, China; 2. Affiliated Hospital of Gansu College of Traditional Chinese Medicine, Lanzhou 730020, Gansu, China

Received:2014-02-20 Online:2014-06-16 Published:2014-06-16

摘要/Abstract

摘要： 目的观察变应性鼻炎(allergic rhinitis，AR)大鼠鼻黏膜上皮细胞线粒体的改变及时相特点和18β-甘草次酸对线粒体病理改变的影响。方法将96只Wistar大鼠随机分为空白组、模型组、氯雷他定组、甘草次酸组。卵清蛋白(ovalbumin，OVA)致敏建立大鼠AR模型。建模成功后给药干预，并分别于给药2、4、6、10周比较各组电镜下鼻黏膜上皮细胞线粒体的改变。结果 AR模型组见线粒体数量增多、体嵴消失、肿胀、空泡化及自噬体存在，且在变应原持续作用下，模型组鼻黏膜上皮细胞线粒体上述损害呈进行性加重。而甘草次酸干预组线粒体在增多的数量、体嵴减少、肿胀、空泡化及自噬体出现均较模型组好转，并随观察周期延长基本接近空白对照组。结论持续变应原刺激下的鼻黏膜上皮细胞线粒体呈进行性损害，18β-甘草次酸可抑制鼻黏膜上皮细胞线粒体的病理损害。

关键词: 鼻炎, 变应性；18β-甘草次酸；线粒体

Abstract: Objective To observe the mitochondria dynamic changes of nasal mucosa epithelial cells in rats with allergic rhinitis and the influence of 18β- glycyrrhetinic acid on them. Methods Ninety-six Wistar rats were randomly divided into 4 groups: the normal group， the model group， the loratadine group and the glycyrrhetinic acid group．AR models were established by ovalbumin and each group was respectively treated subsequently. After 2 weeks， 4 weeks， 6 weeks and 10 weeks，the ultrastructure changes were observed and compared. Results Mitochondria in the model group showed that the number of Mitochondria increased， crest disappeared， volume swelled or even cavitated and autophagy could be found. With persistent allergen exposure，the morphological changes aggravated gradually in the model group，meanwhile the changes in the glycyrrhetinic acid group(mentioned above in the model group)were improved and the mitochondria were much closer to the normal group with the extension of observation. Conclusion Mitochondria ultrastructure changes of nasal mucosa ciliated epithelial cells in the rat with AR exacerbated gradually due to persistent exposure to allergen and 18β-glycyrrhetinic acid is effective to relieve the mitochondria ultrastructure changes.

Key words: -Glycyrrhetinic acid； Mitochondria, Rhinitis, allergic； 18&beta

中图分类号:

R765.21

董明1，陈小婉1,2，席克虎1,2，王有虎1,2，桂岩1,2，张福宏1,2，马春霞1，洪浩1，刘翔毅2，江英1，马毅1，杨贵军1，张小兵1,2. 18β-甘草次酸对变应性鼻炎大鼠鼻黏膜上皮细胞线粒体的影响[J]. 山东大学耳鼻喉眼学报, 2014, 28(3): 1-6.

DONG Ming1, CHEN Xiao-wan1,2, XI Ke-hu1,2, WANG You-hu1,2, GUI Yan1,2, ZHANG Fu-hong1,2, MA Chun-xia1, HONG Hao1, LIU Xiang-yi2, JIANG Ying1, MA Yi1, YANG Gui-jun1, ZHANG Xiao-bing1,2.. Influence of 18-βglycyrrhetinic acid on mitochondria of nasal mucosa epithelial cells in rats with allergic rhinitis[J]. JOURNAL OF SHANDONG UNIVERSITY (OTOLARYNGOLOGY AND OPHTHALMOLOGY), 2014, 28(3): 1-6.

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18β-甘草次酸对变应性鼻炎大鼠鼻黏膜上皮细胞线粒体的影响

Influence of 18-βglycyrrhetinic acid on mitochondria of nasal mucosa epithelial cells in rats with allergic rhinitis

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