山东大学耳鼻喉眼学报 ›› 2015, Vol. 29 ›› Issue (2): 31-35.doi: 10.6040/j.issn.1673-3770.0.2014.352

• 论著 • 上一篇    下一篇

表皮生长因子与其受体在鼻息肉上皮中的表达

段晨1, 徐欣2, 史丽3   

  1. 1. 山东大学医学院, 山东 济南 250012;
    2. 青岛开泰耳鼻喉头颈外科医院, 山东 青岛 266071;
    3. 山东大学齐鲁医院耳鼻咽喉科, 山东 济南 250012
  • 收稿日期:2014-11-05 出版日期:2015-04-16 发布日期:2015-04-16
  • 作者简介:段晨。E-mail:duanchen0627@126.com
  • 基金资助:
    国家自然科学基金(81170897)

Expressions of EGF and EGFR in nasal polyps epithelium

DUAN Chen1, XU Xin2, SHI Li3   

  1. 1. School of Medicine, Shandong University, Jinan 250012, Shandong, China;
    2. Qingdao Caretaker Otolaryngology Head & Neck Surgery Hospital, Qingdao 266071, Shandong, China;
    3. Department of Otolaryngology, Qilu Hospital of Shandong University, Jinan 250012, Shandong, China
  • Received:2014-11-05 Online:2015-04-16 Published:2015-04-16
  • Contact: 史丽。E-mail:shili126@sina.com E-mail:shili126@sina.com

摘要: 目的 探讨表皮生长因子(EGF)及其受体(EGFR)在鼻息肉上皮中的表达及其意义。方法 收集鼻息肉标本25例, 正常下鼻甲黏膜组织10例, 采用免疫组化及实时荧光定量PCR技术, 观察EGF与EGFR在鼻息肉和正常下鼻甲黏膜组织中的表达情况。结果 与正常下鼻甲黏膜组织相比, EGF及EGFR的mRNA水平在鼻息肉组织中的表达下降。在免疫组化染色中, EGF及EGFR主要表达在上皮的基底层细胞, 而且在鼻息肉中的表达下降。结论 EGF及EGFR在正常鼻黏膜组织上皮的发生与修复中有着重要的作用。EGF及EGFR在鼻息肉中蛋白水平与mRNA水平表达的下降表明在鼻息肉中上皮修复功能的下调或缺失可能导致或促进鼻息肉的发生。

关键词: 表皮生长因子, 上皮修复, 表皮生长因子受体, 鼻息肉

Abstract: Objective To investigate the expressions of epidermal growth factor (EGF) and epidermal growth factor receptor(EGFR) in nasal polyps (NPs) as well as their potential roles in polyp epithelium. Methods Polyp biopsies were collected from 25 patients with NPs. Inferior turbinates were obtained from 10 subjects with septal deviation, which were served as the control group. EGF and EGFR expression levels were assessed by using immunohistochemistry and real-time quantitative PCR. Results Staining results revealed that EGF and EGFR mainly localized in basal epithelial cells, and their protein levels were significantly lower in NP than in the control. Down-regulation of both EGF and EGFR mRNA levels were further confirmed in NP as compared with control samples. Conclusion Lower expression of EGF and EGFR in epithelium may be involved in the defect of epithelial repair process in NP.

Key words: Epidermal growth factor, Nasal polyps, Epithelial repair, Epidermal growth factor receptor

中图分类号: 

  • R765.4+1
[1] Li Chunwei. Molecular mechanisms underlying the pathogenesis of nasal polyposis and its response to steroid treatment[D].Singapore, National University of Singapore, 2009.
[2] Li C W, Shi L, Yan Y, et al. Gene expression signatures: a new approach to understanding the pathophysiology of chronic rhinosinusitis[J].Curr Aller Asth Rep, 2013, 13(2):209-217.
[3] Li C W, Cheung W, Lin Z B, et al. Oral steroids enhance epithelial repair in nasal polyposis via upregulation of the AP-1 gene network[J].Thoraxm, 2009, 64(4):306-312.
[4] Li C W, Shi L, Zhang K K, et al. Role of p63/p73 in epithelial remodeling and their response to steroid treatment in nasal polyposis[J]. J Aller Clin Immunol, 2011, 127(3):765-772.
[5] Livak K J, Schmittgen T D. Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) method[J].Methods, 2001, 25(4):402-408.
[6] Jin P, Li C W, Lin Z B, et al. Down-regulation of EMP1 is associated with epithelial metaplasia in nasal inverted papilloma[J].Asian Rhinol J, 2013, 1(1):21-25.
[7] 唐强.表皮生长因子的基础研究及在耳鼻咽喉科中的临床应用[J].医学综述, 2014, 20(14):2516-2518. TANG Qiang. Basic study of epidermal growth factor and its clinical application in otorhinolaryngology[J]. Med Recapit, 2014, 20(14):2516-2518.
[8] Burgel P R, Nadel J A. Roles of epidermal growth factor receptor activation in epithelial cell repair and mucin production in airway epithelium[J].Thorax, 2004, 59(11):992-996.
[9] Kato Y, Hamada Y, Ito S, et al.Epidermal growth factor stimulates the recovery of glucose absorption after small bowel[J].J Surg Res, 1998, 80(2):315-319.
[10] Dvorák B, Kolínská J, McWilliam D L, et al. The expression of epidermal growth factor and transforming growth factor α mRNA in the small intestine of suckling rats:organ culture study[J]. FEBS Lett, 1998, 435(1):119-124.[11] Stern L E, Erwin C R, OBrien D P. Epidermal growth factor is critical for intestinal adaptation following small bowel resection[J]. Microsc Res Tech , 2000, 51(2):138-148.
[12] 王明国.表皮生长因子受体[J].国外医学分子生物学分册, 2002, 24(2):82-85. WANG Mingguo. Epidermal growth factor [J]. J Med Molecul Biol, 2002, 24(2):82-85.
[13] Hedman J, Kaprio J, Poussa T. Prevalence of asthma,aspirin intolerance,nasal polyposis and chronic obstructive pulmonary disease in a population-based study1999 Aug, 28(4):717-722[J].Inter J Epidemiol, 1999, 28(4):717-722.
[14] Larsen K, Tos M. The estimated incidence of symptomatic nasal polyps[J].Acta Otolaryngologica, 2002, 122(2):179-182.
[15] Hosemann W, Gode U, Wagner W. Epidemiology, pathophysiology of nasal polyposis, and spectrum of endonasal sinus surgery[J].Am J Otolaryngol, 1994, 15(2):85-98.
[16] Triglia J M, Nicollas R.Nasal and sinus polyposis in children[J].Laryngoscope, 1997, 107(7):963-966.
[17] Pawankar R, Nonaka M.Inflammatory mechanisms and remodeling in chronic rhinosinusitis and nasal polyps[J].Curr Allergy Asthma Rep, 2007, 7(3):202-208.
[18] Rock J R, Randell S H, Hogan B L. Airway basal stem cells: a perspective on their roles in epithelial homeostasis and remodeling[J]. Dis Model Mech, 2010, 3(9-10):546-556.
[19] Hsu J, Avila P C, Kern R C, et al. Genetics of chronic rhinosinusitis: State of the field and directions forward[J]. J Allergy Clin Immunol, 2013, 131(4):977-993.
[20] Randell S H. Airway epithelial stem cells and the pathophysiology of chronic obstructive pulmonary disease[J]. Proc Am Thorac Soc, 2006, 3(8):718-725.
[21] Nguyen K H, Suzuki H, Wakasugi T, et al. Different expressions of erbB1/2 and tight junction proteins in hypertrophic inferior turbinates and nasal polyps[J]. Eur Arch Otorhinolaryngol, 2013, 270(6):945-951.
[22] Ding G Q, Zheng C Q, Bagga S S. Up-regulation of the mucosal epidermal growth factor receptor gene in chronic rhinosinusitis and nasalpolyposis[J]. Arch Otolaryngol Head Neck Surg, 2007, 133(11):1097-1103.
[23] Zaravinos A, Soufla G, Bizakis J,et al. Expression analysis of VEGFA, FGF2, TGFbeta1, EGF and IGF1 in human nasal polyposis[J]. Oncol Rep, 2008, 19(2):385-391.
[24] Burgel P R, Escudier E, Coste A, et al. Relation of epidermal growth factor receptor expression to goblet cell hyperplasia in nasal polyps[J]. J Aller Clin Immunol, 2000, 106(4):705-712.
[25] Hirota N, Risse P A, Novali M, et al. Histamine may induce airway remodeling through release of epidermal growth factor receptor ligands from bronchial epithelial cells[J]. FASEB J, 2012, 26(4):1704-1716.
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