山东大学耳鼻喉眼学报 ›› 2015, Vol. 29 ›› Issue (2): 31-35.doi: 10.6040/j.issn.1673-3770.0.2014.352

• 论著 • 上一篇    下一篇

表皮生长因子与其受体在鼻息肉上皮中的表达

段晨1, 徐欣2, 史丽3   

  1. 1. 山东大学医学院, 山东 济南 250012;
    2. 青岛开泰耳鼻喉头颈外科医院, 山东 青岛 266071;
    3. 山东大学齐鲁医院耳鼻咽喉科, 山东 济南 250012
  • 收稿日期:2014-11-05 出版日期:2015-04-16 发布日期:2015-04-16
  • 作者简介:段晨。E-mail:duanchen0627@126.com
  • 基金资助:
    国家自然科学基金(81170897)

Expressions of EGF and EGFR in nasal polyps epithelium

DUAN Chen1, XU Xin2, SHI Li3   

  1. 1. School of Medicine, Shandong University, Jinan 250012, Shandong, China;
    2. Qingdao Caretaker Otolaryngology Head & Neck Surgery Hospital, Qingdao 266071, Shandong, China;
    3. Department of Otolaryngology, Qilu Hospital of Shandong University, Jinan 250012, Shandong, China
  • Received:2014-11-05 Online:2015-04-16 Published:2015-04-16
  • Contact: 史丽。E-mail:shili126@sina.com E-mail:shili126@sina.com

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摘要： 目的 探讨表皮生长因子(EGF)及其受体(EGFR)在鼻息肉上皮中的表达及其意义。方法 收集鼻息肉标本25例, 正常下鼻甲黏膜组织10例, 采用免疫组化及实时荧光定量PCR技术, 观察EGF与EGFR在鼻息肉和正常下鼻甲黏膜组织中的表达情况。结果 与正常下鼻甲黏膜组织相比, EGF及EGFR的mRNA水平在鼻息肉组织中的表达下降。在免疫组化染色中, EGF及EGFR主要表达在上皮的基底层细胞, 而且在鼻息肉中的表达下降。结论 EGF及EGFR在正常鼻黏膜组织上皮的发生与修复中有着重要的作用。EGF及EGFR在鼻息肉中蛋白水平与mRNA水平表达的下降表明在鼻息肉中上皮修复功能的下调或缺失可能导致或促进鼻息肉的发生。

关键词: 表皮生长因子, 上皮修复, 表皮生长因子受体, 鼻息肉

Abstract: Objective To investigate the expressions of epidermal growth factor (EGF) and epidermal growth factor receptor(EGFR) in nasal polyps (NPs) as well as their potential roles in polyp epithelium. Methods Polyp biopsies were collected from 25 patients with NPs. Inferior turbinates were obtained from 10 subjects with septal deviation, which were served as the control group. EGF and EGFR expression levels were assessed by using immunohistochemistry and real-time quantitative PCR. Results Staining results revealed that EGF and EGFR mainly localized in basal epithelial cells, and their protein levels were significantly lower in NP than in the control. Down-regulation of both EGF and EGFR mRNA levels were further confirmed in NP as compared with control samples. Conclusion Lower expression of EGF and EGFR in epithelium may be involved in the defect of epithelial repair process in NP.

Key words: Epidermal growth factor, Nasal polyps, Epithelial repair, Epidermal growth factor receptor

中图分类号: 

  • R765.4+1
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