山东大学耳鼻喉眼学报 ›› 2016, Vol. 30 ›› Issue (1): 36-39.doi: 10.6040/j.issn.1673-3770.0.2015.377

• 论著 • 上一篇    下一篇

黏膜接触点性头痛患者接触点鼻黏膜组织中 NK-1受体的表达

赵国敏1,彭洪2,王京3,尹金淑1,2   

  1. 1. 北京大学第九临床医学院, 北京 100038;
    2.首都医科大学附属北京世纪坛医院耳鼻喉头颈外科, 北京 100038;
    3. 首都医科大学附属北京世纪坛医院中心实验室, 北京 100038
  • 收稿日期:2015-09-02 出版日期:2016-02-16 发布日期:2016-02-16
  • 通讯作者: 尹金淑. E-mail:yinjinshu55@aliyun.com E-mail:zhaoguomin1990@126.co
  • 作者简介:赵国敏. E-mail: zhaoguomin1990@126.co
  • 基金资助:
    北京市教育委员会科研计划面上项目(KM201310025026)

The expression of neurokinin-1 receptors in nasal mucosa of patients with intranasal mucosal contact point headaches.

ZHAO Guomin1, PENG Hong2, WANG Jing3, YIN Jinshu1,2.   

  1. 1. Peking University Ninth School of Clinical Medicine, Beijng 100038, China;2. Department of Otorhinolaryngology-Head and Neck Surgery, Beijing Shijitan Hospital of Capital Medical University, Beijing 100038, China;3.Central Laboratory, Beijing Shijitan Hospital of Capital Medical University, Beijing 100038, China
  • Received:2015-09-02 Online:2016-02-16 Published:2016-02-16

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摘要： 目的 探讨NK-1受体(NK-1R)在黏膜接触点性头痛患者接触点鼻黏膜组织中的表达情况。 方法 采用免疫组织化学方法检测NK-1R在18例黏膜接触点性头痛患者接触点和非接触点鼻黏膜组织中的表达,应用逆转录聚合酶链反应(RT-PCR)技术检测部分NK-1R表达阳性的接触点和相应非接触点鼻黏膜组织中的NK-1R和P物质(SP)的mRNA水平。 结果 NK-1R主要表达于接触点鼻黏膜组织的腺泡上皮细胞、炎性细胞和神经纤维。与非接触点鼻黏膜组织相比,接触点鼻黏膜组织中的NK-1R和SP的mRNA水平显著上调。 结论 NK-1R在接触点鼻黏膜组织内的表达可促进痛觉信号的传递,可能参与黏膜接触点性头痛的发生,SP可能是其传递疼痛信息的重要介质。

关键词: NK-1受体, 黏膜接触点性头痛, P物质

Abstract: Objective To investigate the expression of neurokinin-1 receptor(NK-1R)in nasal mucosa tissue from patients with intranasal mucosal contact point headaches. Methods The expression of NK-1R was detected by immunohistochemical staining method in mucosal contact points and non-contact point from 18 patients respectively. The mRNA level of NK-1R and substance P(SP)was detected by real-time quantitative reverse transcription polymerase chain reaction(RT-PCR)at NK-1R positive cells at mucosal contact point and non-contact point respectively. Results NK-1R was mainly located in acinar epithelial cells, inflammatory cells and nerve fiber of contact point nasal mucosa. The mRNA of SP and NK-1R were up-regulated in mucosal contact point compared with those in mucosal non-contact point. Conclusion NK-1R, as a mediator of pain transmission, may play an important role in the occurrence of intranasal mucosal contact point headaches.

Key words: Mucosal contact point headaches, Substance P, Neurokinin-1 receptor

中图分类号: 

  • R765.25
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