羟基红花黄色素A对大鼠视网膜光损伤的保护作用

doi:10.6040/j.issn.1673-3770.0.2022.455

摘要/Abstract

摘要： 目的研究羟基红花黄色素A(hydroxysafflor yellow A,HSYA)对大鼠视网膜光损伤后的保护作用。方法将30只Sprague-Dawley大鼠随机分为空白对照组、模型对照组及HSYA组,每组各10只,后两组通过自制光损伤装置建立大鼠视网膜光损伤模型后,分别腹腔注射生理盐水、HSYA,1次/d,连续14 d,结束后摘除大鼠右眼球制病理切片,通过HE染色观察大鼠视网膜形态,免疫组织化学检测活性Caspase-3蛋白表达水平,TUNEL染色法观察并计算凋亡指数。结果 HE染色结果显示空白对照组大鼠视网膜组织形态和细胞结构正常,模型对照组大鼠视网膜组织形态紊乱,神经节细胞层、内核层、外核层细胞分散不均且大量细胞崩解消融,光感受器细胞内、外节分界不清,视网膜色素上皮(retinal pigment epithelium, RPE)层紊乱,HSYA组大鼠视网膜组织形态大致正常,未见明显损伤性改变。免疫组织化学检测模型对照组Caspase-3蛋白积分光密度值高于空白对照组,而HSYA组Caspase-3蛋白积分光密度值低于模型对照组,差异均有统计学意义(P<0.001)。TUNEL染色结果示模型对照组凋亡指数高于空白对照组(P<0.001),而HSYA组凋亡指数低于模型对照组(P=0.021),差异均具有统计学意义。结论 HSYA可改善光损伤所致的大鼠视网膜组织结构异常改变,降低Caspase-3蛋白的表达,抑制光损伤后大鼠视网膜细胞的凋亡,对视网膜光损伤具有一定的保护作用。

关键词: 视网膜光损伤, 羟基红花黄色素A, Caspase-3蛋白, 细胞凋亡

Abstract: Objective To investigate the protective effect of hydroxysafflor yellow A(HSYA)on light-induced retinal injury in rats. Methods Thirty Sprague-Dawley rats were randomly divided into the blank control group, model control group, and HSYA group(10 rats per group). The rats in the latter two groups were treated with 0.9% NaCl solution and 5.0 mg/mL HSYA, respectively, once a day for 14 days. At the end of the experiment, the pathological sections of the right eye were removed and examined. The morphology of the retina was observed after HE staining, the expression of active caspase-3 protein was detected by immunohistochemistry, and the apoptotic index was calculated using TUNEL staining. Results HE staining showed that the retinal tissue morphology and cell structure of the blank control group were normal. In the model control group, the retinal tissue morphology was disordered, the cells of the RGC, INL, and ONL layers were unevenly dispersed, a large number of cells were disintegrated, the boundary between the inner and outer segments of the photoreceptor cells was not clear, and the retinal pigment epithelium(RPE)layer was disordered. The morphology of the retina in the HSYA group was generally normal, and no obvious damage was observed. The integrated optical density of caspase-3 protein detected by immunohistochemistry was significantly higher in the model control group than in the blank control group(P<0.001), while the integrated optical density of caspase-3 protein in the HSYA group was significantly lower than in the model control group(P<0.001). TUNEL staining showed that the apoptotic index of the model control group was significantly higher than that of the blank control group(P<0.001), while the apoptotic index of the HSYA group was significantly lower than that of the model control group(P=0.021), and the differences were statistically significant. Conclusion HSYA improved abnormal changes in the retinal tissue structure caused by light damage in rats, reduce the expression of caspase-3 protein, and inhibit the apoptosis of retinal cells after light damage. Based on these findings, HSYA has a certain protective effect against retinal light damage.

Key words: Light-induced retinal injury, Hydroxysafflor yellow A, Caspase-3 protein, Apoptosis

中图分类号:

R774.1

付艺璇,王涛,曹静,李光达,郑凌方,许莞菁,赵爽. 羟基红花黄色素A对大鼠视网膜光损伤的保护作用[J]. 山东大学耳鼻喉眼学报, 2023, 37(5): 128-134.

FU Yixuan, WANG Tao, CAO Jing, LI Guangda, ZHENG Lingfang, XU Wanjing, ZHAO Shuang. The protective effect of hydroxysafflor yellow A on light-induced retinal injury in rats[J]. Journal of Otolaryngology and Ophthalmology of Shandong University, 2023, 37(5): 128-134.

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