山东大学耳鼻喉眼学报 ›› 2024, Vol. 38 ›› Issue (4): 55-61.doi: 10.6040/j.issn.1673-3770.0.2023.146
• 论著 • 上一篇
倪荣生1,沈晓辉2,高下2
NI Rongsheng1, SHEN Xiaohui2, GAO Xia2
摘要: 目的 分析喉鳞状上皮细胞癌(laryngeal squamous cell carcinoma, LSCC)中基质金属蛋白酶(matrix metalloproteinase, MMPs)家族及组织金属蛋白酶抑制剂(tissue inhibitors of matrix metalloproteinases, TIMPs)的基因表达谱,找出与LSCC相关性最高的基因进行进一步研究,探讨其蛋白的表达与临床病理特征相关性。 方法 选取28例LSCC患者,采用含人类全基因组的基因芯片来检测MMPs及TIMPs在4例LSCC患者中共同上调或下调的基因。采用逆转录聚合酶链反应(reverse transcription-polymerase chain reaction, RT-PCR)及western blot检测24例患者该基因和蛋白的表达来验证基因芯片结果,并研究该蛋白的表达与临床病理特征之间的关系。 结果 基因芯片显示MMPs家族在LSCC与癌旁正常细胞之间的差异表达基因有14个,其中4个基因仅表达上调,4个基因仅表达下调,另有6个基因在部分患者中表达上调,而在另一部分患者中表达下调。未发现在4例患者中共同表达上调或下调的基因。TIMPs在LSCC与癌旁正常细胞之间全部表达下调,TIMP4在4例患者中全部表达下调。RT-PCR检测结果显示TIMP4基因在LSCC组织中低表达。western blot检测结果显示TIMP4蛋白在LSCC组织中低表达,且TIMP4蛋白的表达与淋巴结转移相关。 结论 研究首次发现MMPs家族及TIMPs在LSCC的表达谱,并发现TIMP4蛋白的表达与LSCC临床病理特征相关。未来TIMP4蛋白可能作为LSCC潜在的治疗靶点。
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