不同发育时期小鼠初级听觉皮层中芳香化酶及雌激素受体表达与听力的相关性

doi:10.6040/j.issn.1673-3770.0.2024.517

摘要/Abstract

摘要： 目的探讨初级听皮层(primary auditory cortex, A1)中芳香化酶(aromatase, AROM)及三种雌激素受体(estrogen receptors, ERs):雌激素受体α(estrogen receptor alpha, ERα)、雌激素受体β(estrogen receptor beta, ERβ)和G蛋白偶联受体30(G protein-coupled receptor, GPR30)在听觉发育中的表达及作用。方法选取出生后7日龄(P7)、14日龄(P14)、21日龄(P21)、30日龄(P30)和60日龄(P60)的C57BL/6J雄性小鼠,采用听觉脑干诱发电位(auditory brainstem response, ABR)检测听阈;采用qRT-PCR、Western bloting、免疫组化等检测小鼠A1内AROM和ERs表达变化;进行表达量与听阈的Spearman相关性分析。结果 ABR从P14可检测后持续下降(P<0.05),于P30达到成熟水平。qPCR显示AROM、GPR30和ERβ表达随日龄递增(P<0.05);ERα在P30时达到峰值(P<0.05)。蛋白水平(Western bloting和免疫组化)实验的结果表明AROM和ERs的表达量随发育显著升高(P<0.05)。Spearman相关性分析结果表明,AROM、ERβ的表达与听力阈值的发育变化为强相关(∣r_s∣分别为0.75、0.72),GPR30呈弱相关(∣r_s∣为0.64),ERα无显著相关性。结论小鼠出生后听力逐渐发育完善,A1内AROM及GPR30、ERβ表达水平逐渐升高,提示AROM-GPR30/ERβ信号轴可能促进了小鼠听觉功能的建立和成熟。

关键词: 芳香化酶, 雌激素, 雌激素受体, 雌激素受体α, 雌激素受体β, G蛋白偶联受体30, 听皮层

Abstract: Objective To investigate the expression and potential regulatory roles of aromatase(AROM)and three estrogen receptors(ERs)—estrogen receptor alpha(ERα), estrogen receptor beta(ERβ), and G protein-coupled receptor 30(GPR30)—in the primary auditory cortex(A1)during auditory development. Methods Male C57BL/6J mice at different postnatal stages—postnatal day 7(P7), P14, P21, P30, and P60-were selected. Auditory thresholds were measured by auditory brainstem response(ABR), and qRT-PCR, Western bloting, and immunohistochemistry were performed to examine the expression changes of AROM and ERs in the A1. Spearman correlation analysis was conducted between expression levels and hearing thresholds. Results ABR thresholds decreased significantly from P14 onward(P<0.05), reaching maturity at P30. qPCR revealed that the expression of AROM, GPR30, and ERβ increased with age(P<0.05)while ERα peaked at P30(P<0.05). Protein levels(Western bloting and immunohistochemistry)confirmed a significant developmental increase in AROM and ERs expression(P<0.05). Spearman correlation analysis showed strong correlations between the developmental changes in hearing thresholds and the expression of AROM(∣r_s∣=0.75)and ERβ(∣r_s∣=0.72), a weak correlation with GPR30(∣r_s∣=0.64), and no significant correlation with ERα. Conclusion Postnatal auditory development in mice progressively matures, accompanied by increasing expression levels of AROM, GPR30, and ERβ in the A1. The AROM-GPR30/ERβ axis is suggested to potentially facilitate the establishment and maturation of auditory function in mice.

Key words: Aromatase, 17β-Estradiol, Estrogen receptors, ERα, ERβ, G protein coupled receptor 30, Auditory cortex

中图分类号:

R339.16

唐茜,黄茂凌,张吉强,钟诚. 不同发育时期小鼠初级听觉皮层中芳香化酶及雌激素受体表达与听力的相关性[J]. 山东大学耳鼻喉眼学报, 2025, 39(6): 46-53.

TANG Xi, HUANG Maoling, ZHANG Jiqiang, ZHONG Cheng. Correlation between aromatase and estrogen receptor expression in the primary auditory cortex of mice at different developmental stages and hearing ability[J]. Journal of Otolaryngology and Ophthalmology of Shandong University, 2025, 39(6): 46-53.

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