山东大学耳鼻喉眼学报 ›› 2008, Vol. 22 ›› Issue (1): 1-05 .

• 论文 •    下一篇

茶多酚防治STZ诱导的大鼠糖尿病性白内障的机制

毕宏生,李树杰,崔 彦,王 慧   

  1. 山东中医药大学眼科中心 山东施尔明眼科医院, 济南 250002
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2008-02-24 发布日期:2008-02-24
  • 通讯作者: 毕宏生

Effect of tea polyphenols in preventing diabetic cataracts induced by streptozotocin in rats and its mechanism

BI Hong-sheng, LI Shu-jie, CUI Yan, WANG Hui   

  1. Eye Center of Shandong University of Traditional Chinese Medicine,Shandong Shierming Eye Hospital, Jinan 250002, China
  • Received:1900-01-01 Revised:1900-01-01 Online:2008-02-24 Published:2008-02-24
  • Contact: BI Hong-sheng

摘要:

目的探讨茶多酚对STZ诱导的大鼠糖尿病性白内障防治的作用机制。方法制备STZ糖尿病大鼠模型。成模后(血糖>16.7mmol/L)将大鼠分为正常组、STZ诱导糖尿病组(STZ-DM)和茶多酚干预糖尿病组(TP-DM)。TP-DM组每只大鼠每日给予100mL茶多酚溶液,正常组和STZ-DM组大鼠饮用自来水,所有大鼠正常饮食。HE染色观察晶状体细胞的形态学改变;采用裂隙灯显微镜观察并记录晶状体混浊的程度;使用分光光度计测定晶状体中还原型谷胱甘肽的含量和谷胱甘肽还原酶、过氧化氢酶的活性;大鼠尾静脉取血测定血液中糖化血红蛋白、甘油三酯含量。结果HE染色可见,STZ-DM组大鼠晶状体赤道部至后囊区域内晶状体纤维细胞可见大量未被降解的细胞器,无细胞器区域缩小。建立糖尿病大鼠模型后6~9周,与STZ-DM组大鼠相比,TP-DM组大鼠晶状体混浊程度降低0.5~1个级别;13周时,还原型谷胱甘肽的含量升高45%,过氧化氢酶的活性增强174%,差别具有统计学意义(P<0.05)。另外,TP-DM组大鼠与同时期STZ-DM组大鼠相比,体重降低(P<0.05);TP-DM组和STZ-DM组大鼠血液中糖化血红蛋白、甘油三酯含量均高于正常对照组,差别具有统计学意义(P<0.05)。结论晶状体纤维细胞的异常细胞器降解可能与糖尿病性白内障的发生密切相关;氧化损伤可能在糖尿病性白内障的发生中起重要作用;茶多酚溶液对糖尿病性白内障的形成有抑制作用,可能是通过茶多酚抑制晶状体的氧化损伤而延缓早期的糖尿病大鼠晶状体混浊。

关键词: 糖尿病性白内障;链脲佐菌素;茶多酚;氧化应激

Abstract:

To explore the mechanism of diabetic cataracts induced by streptozotocin in rats and to investigate the role of tea polyphenols(TP) in preventing diabetic cataracts. MethodsDiabetic cataracts were induced by streptozocin(STZ). The diabetic rats (blood glucose>16.7mmol/L) were randomly divided into 3 groups: the TP-treatment group(TP-DM), the untreated diabetic group (STZ-DM) and the control group. Each rat of the TP-DM group received 100 ml TP solution per day and of the control and the STZ-DM groups received tap water besides normal diets. Some lenses were stained with HE and examined with light microscopy after 12 weeks. Progression of lens opacification was recorded using a slit lamp at regular time intervals. The levels of glutathione reductase(GR), catalase(CAT) and glutathione(GSH) were determined spectrometically. The glycosylated hemoglobin and triglyceride(TG) of the blood of the vene caudalis were determined. ResultsMorphological changes markedly occurred in diabetic cataracts. The TP treatment delayed the progression of cataracts in diabetic rats and the delay was statistically significant from the 6th to the 9th week of diabetes (about 0.51 grade, P<0.05 vs the untreated diabetic rats). An increase in CAT and GSH was found in the lenses of the TP treated diabetic rats (P<0.05 vs the untreated diabetic rats) after 12 weeks of diabetes. Moreover, a decrease in body weight and an increase in glycosylated hemoglobin and triglyceride(TG) were found in the blood of the vene caudalis of the diabetic rats (P<0.05 vs the normal control rats). Conclusions The abnormal degradation of lens fibrocytes may be the morphological base of diabetic cataracts. The oxidative damage may play an important role in diabetic cataracts. TP can delay the progression of lens opacification only during the early stages, which may be related to the protective effect of TP against inactivation of anti-oxidation enzymes.

Key words: Diabetic cataracts; Streptozocin; Tea polyphenols; Oxidative stress

中图分类号: 

  • R776.1
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