鼻息肉中核因子κB亚单位P50活性与IL-4、γ-IFN因子表达的关系

山东大学耳鼻喉眼学报

鼻息肉中核因子κB亚单位P50活性与IL-4、γ-IFN因子表达的关系

李云秋¹，冯永²，王继华¹

1. 湖南省人民医院耳鼻咽喉-头颈外科，长沙 410002； 2. 中南大学湘雅医院耳鼻咽喉-头颈外科，长沙 410002

收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2008-02-24 发布日期:2008-02-24
通讯作者: 李云秋

Correlation between nuclear factor-kappa B subunit P50 activity and IL-4 and γ-IFN expressions in nasal polyps

LI Yun-qiu¹, FENG Yong², WANG Ji-hua¹

Department of Otolaryngology Head and Neck Surgery, 1. Hunan Provincial Hospital, Changsha 410002, China;2. Xiangya Hospital of Central South University, Changsha 410002, China

Received:1900-01-01 Revised:1900-01-01 Online:2008-02-24 Published:2008-02-24
Contact: LI Yun-qiu

摘要/Abstract

摘要： 目的测定鼻息肉中核因子κB亚单位P50的活性及其与IL-4、γ-IFN表达的关系，探讨P50对TH1/TH2细胞因子表达调节的可能机制。方法采用SP免疫组化染色法检测40例鼻息肉患者与20例正常钩突黏膜中IL-4、γ-IFN、P50的表达,以核染阳性率来评估P50活性。对P50活性与IL-4,γ-IFN因子行直线相关分析。结果①鼻息肉中P50胞浆、胞核均有阳性表达,主要位于上皮细胞、炎症细胞及腺上皮细胞胞浆,P50活性显著增强(P<0.001)，IL-4表达亦明显增强(P<0.001), γ-IFN表达无明显改变(P>0.05)；②Pearson相关性分析提示，P50活性与IL-4表达呈直线正相关关系(r=0.70, P<0.001),而与γ-IFN表达无相关性(r=0.14, P=0.40)。结论鼻息肉中P50的活性明显增强，P50的激活上调IL-4的表达可能是鼻息肉中Th1/Th2因子失衡的重要环节之一。

关键词: 鼻息肉, NF-κB/P50, 细胞因子, IL-4, γ-IFN

Abstract: To explore the possible regulatory mechanism of nuclear factorkappa B subunit P50 to Th1/Th2 cytokine in nasal polyps. MethodsThe expressions of IL-4 , γ-IFN and P50 were determined by the method of immunohistochemistry staining in 40 cases of nasal polyps and 20 normal subjects, and the linear correlations between activities of P50 and IL-4 and γ-IFN were evaluated. ResultsThe expression of P50 was mainly distributed in the cytoplasm of epithelia, inflammatory cells and the epithelia of glands, and the expression of IL-4 and the activity of P50 were significantly increased in nasal polyps(both P<0.001), however, the expression of γ-IFN had no significant differences between the groups(P>0.05). The Pearson correlation analysis demonstrated that P50 nuclear-presence closely correlated with the IL-4 level, but not with γ-IFN. The correlation coefficient was 0.70 for P50 and IL-4(P<0.001 ) and 0.14 for P50 and γ-IFN (P>0.05). ConclusionThe activity of P50 in nasal polyps significantly increases, and it may play an important role in the imbalance of Th1/Th2-typed cytokines by up-regulating the level of IL-4.

Key words: Nuclear factor κB sub-unit P50, Cytokine, Nasal polyps, IL-4, γ-IFN

中图分类号:

R765.25

李云秋,冯永,王继华 . 鼻息肉中核因子κB亚单位P50活性与IL-4、γ-IFN因子表达的关系[J]. 山东大学耳鼻喉眼学报, 2008, 22(1): 48-51 .

LI Yun-qiu,FENG Yong,WANG Ji-hua . Correlation between nuclear factor-kappa B subunit P50 activity and IL-4 and γ-IFN expressions in nasal polyps[J]. JOURNAL OF SHANDONG UNIVERSITY (OTOLARYNGOLOGY AND OPHTHALMOLOGY), 2008, 22(1): 48-51 .

参考文献

相关文章 15

[1]	宋晓宇, 张宇, 李成林, 宋西成. 加速康复外科理念在慢性鼻-鼻窦炎伴鼻息肉手术治疗中的应用[J]. 山东大学耳鼻喉眼学报, 2018, 32(5): 9-12.
[2]	黄远,巴罗,李波,余文兴,杨芳丽,杜进涛. 慢性鼻窦炎鼻息肉中转录因子NKX2-1调控嗜酸性炎症反应的作用[J]. 山东大学耳鼻喉眼学报, 2018, 32(4): 17-23.
[3]	陈望兴,赵雪,刘岩,刘悦,金春顺. 鼻中隔偏曲及慢性鼻窦炎鼻息肉患者的精神心理状态调查[J]. 山东大学耳鼻喉眼学报, 2018, 32(4): 24-27.
[4]	黄凯丰. 糖皮质激素短疗程雾化吸入对嗜酸粒细胞性鼻窦炎伴鼻息肉患者鼻部症状及肾上腺皮质功能的影响[J]. 山东大学耳鼻喉眼学报, 2018, 32(4): 28-31.
[5]	李华斌, 赖玉婷. 慢性鼻-鼻窦炎的发病机制及诊疗进展[J]. 山东大学耳鼻喉眼学报, 2018, 32(3): 4-9.
[6]	于倩倩,关鸽,张念凯,李娜,温君凤,刘婷婷,连媛媛. 鼻腔嗅裂区呼吸道上皮腺瘤样错构瘤的CT影像表现[J]. 山东大学耳鼻喉眼学报, 2018, 32(3): 76-81.
[7]	李宏梅,王兰,高照慧,王莹,薛金梅. p-ERK2及ERK2在鼻息肉组织的表达及其意义[J]. 山东大学耳鼻喉眼学报, 2018, 32(3): 42-46.
[8]	张劼,龚齐. 不同分型慢性鼻-鼻窦炎患者外周血嗜酸性粒细胞与中性粒细胞百分比及变态反应临床差异性分析[J]. 山东大学耳鼻喉眼学报, 2018, 32(3): 54-57.
[9]	甘彬,张永举,许安廷. BRF2在慢性鼻-鼻窦炎伴鼻息肉发病过程中的作用研究[J]. 山东大学耳鼻喉眼学报, 2018, 32(3): 47-53.
[10]	郅莉莉,宋道亮. 嗜酸性粒细胞及IL5在上颌窦后鼻孔息肉与鼻息肉中表达的差异[J]. 山东大学耳鼻喉眼学报, 2017, 31(4): 43-46.
[11]	伦杰,张亚楠. 慢性鼻-鼻窦炎伴鼻息肉复发的相关影响因素分析[J]. 山东大学耳鼻喉眼学报, 2017, 31(3): 80-83.
[12]	童尧,郑岩,周雅丽,王艺晓,赵培泉,汪朝阳. 各型青光眼患者眼内TNF-α及IP-10水平及其相关性分析[J]. 山东大学耳鼻喉眼学报, 2017, 31(1): 103-106.
[13]	刘叶,孙殿水,殷进军,夏明,徐英,孙建华. 鼻咽癌调强放疗后前庭功能损伤的机制研究[J]. 山东大学耳鼻喉眼学报, 2016, 30(6): 14-17.
[14]	包扬,王春玲,张丽,金敏,周长青,潘新良. 帕瑞昔布钠联合曲马多镇痛对悬雍垂腭咽成形术患者的影响[J]. 山东大学耳鼻喉眼学报, 2016, 30(5): 49-53.
[15]	王海鹏,徐会娟,张秀芳,王惠忠,李延忠. 塞纳斯凝胶在鼻息肉伴睡眠呼吸障碍成年患者鼻内镜术中的应用[J]. 山东大学耳鼻喉眼学报, 2016, 30(5): 58-60.

多维度评价

Viewed

Full text

Abstract

Cited

Shared

Discussed