睾酮对喉癌HEP-2细胞株分裂增殖及端粒酶活性的影响

山东大学耳鼻喉眼学报 ›› 2009, Vol. 23 ›› Issue (1): 15-17 .

睾酮对喉癌HEP-2细胞株分裂增殖及端粒酶活性的影响

王洪波¹，田军²，李红梅³

（1. 潍坊医学院耳鼻喉教研室； 2. 潍坊市人民医院耳鼻喉科；
3. 潍坊医学院耳鼻喉教研室，山东潍坊 261000）

收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2009-02-16 发布日期:2009-02-16
通讯作者: 田军

Effects of testosterone on proliferation and telomerase activity of Hep2 cells

WANG Hongbo¹,　TIAN Jun²,　LI Hongmei³

(1. Department of Otolaryngology， Weifang Medical College； 2. Department of Otolaryngology， Weifang People′s Hospital；
3. Department of Otolaryngology， Weifang Medical College, Weifang 261000, Shandong, China)

Received:1900-01-01 Revised:1900-01-01 Online:2009-02-16 Published:2009-02-16
Contact: TIAN Jun

摘要/Abstract

摘要：

目的研究睾酮对人喉癌HEP2细胞株分裂增殖及端粒酶表达活性的影响。方法采用噻唑蓝（MTT）比色法研究不同浓度睾酮对Hep2细胞株分裂增殖的影响，用免疫细胞化学检测10-8mol/L睾酮作用48?h细胞雄激素受体及端粒酶的表达活性。结果10-8mol/L睾酮作用48?h，人喉癌细胞Hep2细胞OD值由作用前的0.150±0.008增至0.281±0.010，其他实验浓度组均不能明显促进Hep2细胞的分裂增殖。睾酮作用前后Hep2细胞均强表达雄激素受体，表达率分别为95.73%和93.84%，差别无统计学意义。端粒酶表达率由睾酮作用前的52.35%增至89.21%，端粒酶强阳性细胞爬片由2张增至8张，差异有统计学意义。结论HEP2细胞株为雄激素敏感型细胞，睾酮可能通过上调端粒酶的表达促进喉癌Hep2细胞的分裂增殖，这对研究喉癌的激素疗法及开发端粒酶抑制剂有重要的意义。

关键词: 睾酮；Hep2细胞株；端粒酶

Abstract:

To investigate the effects of testosterone on proliferation and telomerase activity of Hep2 cells. MethodsProliferation activity of Hep2 cells was assessed by MTT assay. Expressions of Androgen Receptor antigen and Telomerase antigen were determined at 48?h after testosterone (10-8mol/L ) treatment by an immunocytochemistry method. ResultsOD values of Hep2cells before treatment and at 48?h after testosterone (10-8mol/L) treatment were 0.150±0.008 and 0.281±0.010. Testosterone at a concentration of 10-8mol/L may stimulate proliferation activity of Hep2 cells. Others had no such effect. Testosterone antigen activity was respectively 95.73% and 93.84% before and after testosterone (10-8mol/L) treatment, with no significant changes. However, telomerase activity increased from 52.35% to 89.21%, with obvious statistical significance. Slides with strong expression of telomerase increased from 2 to 8. ConclusionThe Hep2 cell line is androgensensitive cell. Testosterone can stimulate proliferation of Hep2 cells through increasing telomerase activity. Therefore, it has potential to develop endocrine therapy and antitelomerase drugs for laryngeal carcinoma.

Key words: Testosterone; Hep2; Telomerase

中图分类号:

R739.65

王洪波1 ，田军2 ，李红梅3
. 睾酮对喉癌HEP-2细胞株分裂增殖及端粒酶活性的影响[J]. 山东大学耳鼻喉眼学报, 2009, 23(1): 15-17 .

WANG Hongbo¹,　TIAN Jun²,　LI Hongmei³
. Effects of testosterone on proliferation and telomerase activity of Hep2 cells[J]. JOURNAL OF SHANDONG UNIVERSITY (OTOLARYNGOLOGY AND OPHTHALMOLOGY), 2009, 23(1): 15-17 .

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