EGCG对鼻咽癌细胞株裸鼠移植瘤的放疗增敏作用以及对Survivin表达的影响

山东大学耳鼻喉眼学报 ›› 2009, Vol. 23 ›› Issue (1): 6-9 .

EGCG对鼻咽癌细胞株裸鼠移植瘤的放疗增敏作用以及对Survivin表达的影响

雷迅¹，刘强和¹，孔中雨¹，向秋²，耿宛平¹，黄辉³，董译元¹，刘芳贤¹

(1. 桂林医学院附属医院耳鼻咽喉- 头颈外科， 2. 桂林医学院科学实验中心，
3. 桂林医学院附属医院肿瘤放疗科, 广西桂林 541001)

收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2009-02-16 发布日期:2009-02-16

ARelationship between expression of Survivin and radiosensitization effect of EGCG in xenotransplanted nasopharyngeal carcinoma

LEIXun¹, LIU Qianghe¹, KONG Zhongyu¹, XIANG Qiu², GENG WANping¹, HUANG Hui³, DONG Yiyuan¹, LIU Fangxian¹

(1. Department of Otorhinolaryngology & Head and Neck Surgery, Affiliated Hospital of Guilin Medical College;
2. Scientific Research & Experiment Center, Guilin Medical College;
3. Department of Radioncology, Affiliated Hospital of Guilin Medical College, Guilin 541001, Guangxi, China )

Received:1900-01-01 Revised:1900-01-01 Online:2009-02-16 Published:2009-02-16

摘要/Abstract

摘要：

目的研究凋亡抑制基因Survivin的表达与EGCG对鼻咽癌CNE2裸鼠移植瘤放疗增敏作用的关系。方法将低分化鼻咽癌细胞CNE2接种于4～6周龄的雌性裸鼠皮下，待移植瘤生长至4～6?mm时，随机分为4组，分别采用NS、EGCG［50?mg/(kg·w）］、放疗（15?Gy）、EGCG给药24?h后再行放疗等方法分别处理各组裸鼠，处理前后分别测量裸鼠移植瘤的短径、体积。21?d后处死裸鼠，取出肿瘤并称重，将肿瘤组织分成两份，分别用来进行病理切片TUNEL染色以及RTPCR检测Survivin mRNR的表达。结果EGCG＋放疗对裸鼠移植瘤的生长抑制最为明显，肿瘤生长抑制率为89.3%，消退率为40.0％。与对照组EGCG组、放疗组相比，EGCG＋放疗组的凋亡指数均明显增加（P＜0.05)，同时，伴有Survivin mRNR的表达显著下降（P＜0.05）。结论EGCG对鼻咽癌裸鼠移植瘤可能具有放疗增敏作用，这种作用可能与凋亡抑制基因Survivin的表达下调有关。

关键词: 鼻咽肿瘤；肿瘤移植； EGCG；放射疗法； Survivin；小鼠，裸

Abstract:

To investigate the relationship between expression of Survivin and radiosensitization effects of EGCG in the in vivo growth of nasopharyngeal carcinoma. MethodsThe nasopharyngeal carcinoma undifferentiated cell line (CNE2) was subcutaneously inoculated into 20 nude mice (46weeks, female), and the volume and the shorter diameter of tumors were determined. When the shorter diameter of tumors reached 46?mm, NS, EGCG(50?mg/kg·w), radiotherapy(15?Gy), and EGCG(50?mg/kg·w) followed by radiotherapy(15?Gy) 24 hours later, were administered to the animals. The volume and the shorter diameter of tumors were checked. The excised tumor masses on the 21st day after administration were frozen and processed for reverse transcriptionpolymerase chain reaction(RTPCR) to investigate expression of Survivin mRNR. At the same time, one part of each tumor mass was processed for immunohistochemical TUNEL staining to get the apoptosis index(AI). ResultsEGCG in combination with radiotherapy showed a significant inhibitory effect on both tumor growth and expression of Survivin mRNA（P＜0.05). The apoptosis index increased after EGCG treatment in combination with radiotherapy（P＜0.05）. ConclusionEGCG is a good choice for the treatment of nasopharyngeal carcinoma and has radiosensitization on nasopharyngeal carcinoma. Survivin is a molecular agent taking part in the course of apoptosis induced by EGCG in combination with radiotherapy.

中图分类号:

R739.63

雷迅1 ，刘强和1 ，孔中雨1 ，向秋2 ，耿宛平1 ，黄辉3 ，董译元1 ，刘芳贤1
. EGCG对鼻咽癌细胞株裸鼠移植瘤的放疗增敏作用以及对Survivin表达的影响[J]. 山东大学耳鼻喉眼学报, 2009, 23(1): 6-9 .

LEIXun, LIU Qianghe, KONG Zhongyu, XIANG Qiu, GENG WANping, HUANG Hui, DONG Yiyuan, LIU Fangxian. ARelationship between expression of Survivin and radiosensitization effect of EGCG in xenotransplanted nasopharyngeal carcinoma[J]. JOURNAL OF SHANDONG UNIVERSITY (OTOLARYNGOLOGY AND OPHTHALMOLOGY), 2009, 23(1): 6-9 .

参考文献

多维度评价

Viewed

Full text

Abstract

Cited

Shared

Discussed

[1]	邹纪东，徐伟，吕正华，曹洪源 . 喉癌中maspin与p53表达的相关性[J]. 山东大学耳鼻喉眼学报, 2011, 25(6): 16-18.
[2]	王德利，潘新良，雷大鹏. 下咽鳞癌中uPA的表达和微血管密度与肿瘤侵袭转移的关系[J]. 山东大学耳鼻喉眼学报, 2011, 25(2): 23-28.
[3]	朱国臣，张永胜，郑晓彬，肖大江. 乙酰肝素酶、nm23-H1在鼻咽癌中的表达以及与侵袭转移的关系[J]. 山东大学耳鼻喉眼学报, 2010, 24(4): 30-33.
[4]	梁辉1,2，潘新良1,马超3，王启荣2，韩飞2，崔朝阳2. 下咽癌FADU细胞系中CD133阳性细胞侵的袭能力[J]. 山东大学耳鼻喉眼学报, 2010, 24(2): 5-7.
[5]	唐智陈勇明王继群. 靶向人端粒酶小干扰RNA对人喉癌Hep-2细胞系形态学影响[J]. 山东大学耳鼻喉眼学报, 2009, 23(5): 4-7.
[6]	黄方康瑞. 鼻咽癌组织中PI3Kp85的异常表达[J]. 山东大学耳鼻喉眼学报, 2009, 23(3): 17-20.