内皮素1对喉癌细胞HEP 2的促增殖作用

山东大学耳鼻喉眼学报 ›› 2009, Vol. 23 ›› Issue (2): 1-4 .

• 论文 • 下一篇

内皮素1对喉癌细胞HEP 2的促增殖作用

徐定远¹,郑雪莲²,吴波³,薛飞¹,李泽卿¹,季俊峰¹,王秋萍¹

(1. 南京军区总医院耳鼻咽喉科, 南京 210002; 2. 四川大学华西第二附属医院分子与转化实验室, 成都 610041；3. 南京军区总医院病理科, 南京 210002)

收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2009-04-16 发布日期:2009-04-16
通讯作者: 王秋萍

Effect of endothelin 1 on mitosis of human HEP 2 cells in vitro

XU Dingyuan¹, ZHENG Xuelian², WU Bo³, XUE Fei¹, LI Zeqinq¹, JI Junfeng¹, WANG Qiuping¹

(1. Department of Otorhinolaryngology, Nanjing General Hospital of Nanjing Military Command, Chinese PLA, Nanjing 210002, China; 2. Molecular and Translational Medicine Lab, Second Affiliated Hospital of Sichuan University, Chengdu 610041, China;
3.Department of Pathology, Nanjing General Hospital of Nanjing Military Command, Chinese PLA, Nanjing 210002, China)

Received:1900-01-01 Revised:1900-01-01 Online:2009-04-16 Published:2009-04-16
Contact: WANG Qiuping

摘要/Abstract

摘要：

目的探讨内皮素1对HEP 2细胞有丝分裂的影响。方法采用RTPCR及免疫组化Dako EnVision法检测HEP 2细胞内皮素受体表达情况;3H胸腺嘧啶核苷渗入法检测不同浓度和作用时间内皮素1对HEP 2细胞有丝分裂的影响。结果HEP 2细胞存在内皮素受体基因及蛋白表达;内皮素1具有明显促HEP2细胞增殖作用,且其促增殖作用呈浓度依赖性。结论内皮素1能有效促进HEP 2细胞有丝分裂,对喉癌的演进可能有重要调控作用。

关键词: 关键词:内皮素 1;HEP 2 细胞;有丝分裂

Abstract:

To investigate the effect of endothelin 1 on mitosis of HEP 2 cells. MethodsExpression of endothelin receptors on HEP 2 cells was determined by RTPCR and immunohistochemical methods. HEP 2 cell lines were exposed to different concentrations of the endothelin 1, and mitosis of HEP 2 cells was measured by 3ResultsGene expression of the endothelinreceptor was detected by RTPCR. Immunohistochemical studies showed that endothelin receptor immunoreactivity appeared in HEP 2 cells. Addition of endothelin 1 resulted in an increase of DNA synthesis in concentration dependence. ConclusionEndothelin 1 can promote mitosis of HEP 2 cells and may play an important role in the progression of laryngeal carcinoma.

Key words: Endothelin 1; HEP 2 cells; Mitosis

中图分类号:

R76719

徐定远 ,郑雪莲 ,吴波 ,薛飞,李泽卿,季俊峰 ,王秋萍. 内皮素1对喉癌细胞HEP 2的促增殖作用[J]. 山东大学耳鼻喉眼学报, 2009, 23(2): 1-4 .

XU Dingyuan¹, ZHENG Xuelian², WU Bo³, XUE Fei¹, LI Zeqinq¹, JI Junfeng¹, WANG Qiuping¹

. Effect of endothelin 1 on mitosis of human HEP 2 cells in vitro[J]. JOURNAL OF SHANDONG UNIVERSITY (OTOLARYNGOLOGY AND OPHTHALMOLOGY), 2009, 23(2): 1-4 .

参考文献

多维度评价

Viewed

Full text

Abstract

Cited

Shared

Discussed

内皮素1对喉癌细胞HEP 2的促增殖作用

Effect of endothelin 1 on mitosis of human HEP 2 cells in vitro

PDF (PC)

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 0

多维度评价

本文评价

推荐阅读 0