山东大学耳鼻喉眼学报 ›› 2009, Vol. 23 ›› Issue (2): 1-4 .

• 论文 •    下一篇

内皮素1对喉癌细胞HEP 2的促增殖作用

徐定远1,郑雪莲2,吴波3,薛飞1,李泽卿1,季俊峰1,王秋萍1   

  1. (1. 南京军区总医院耳鼻咽喉科, 南京 210002; 2. 四川大学华西第二附属医院分子与转化实验室, 成都 610041;3. 南京军区总医院病理科, 南京 210002)
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2009-04-16 发布日期:2009-04-16
  • 通讯作者: 王秋萍

Effect of endothelin 1 on mitosis of human HEP 2 cells in vitro

XU Dingyuan1, ZHENG Xuelian2, WU Bo3, XUE Fei1, LI Zeqinq1, JI Junfeng1, WANG Qiuping1   

  1. (1. Department of Otorhinolaryngology, Nanjing General Hospital of Nanjing Military Command, Chinese PLA, Nanjing 210002, China; 2. Molecular and Translational Medicine Lab, Second Affiliated Hospital of Sichuan University, Chengdu 610041, China;
    3.Department of Pathology, Nanjing General Hospital of Nanjing Military Command, Chinese PLA, Nanjing 210002, China)
  • Received:1900-01-01 Revised:1900-01-01 Online:2009-04-16 Published:2009-04-16
  • Contact: WANG Qiuping

摘要:

目的探讨内皮素1对HEP 2细胞有丝分裂的影响。方法采用RTPCR及免疫组化Dako EnVision法检测HEP 2细胞内皮素受体表达情况;3H胸腺嘧啶核苷渗入法检测不同浓度和作用时间内皮素1对HEP 2细胞有丝分裂的影响。结果HEP 2细胞存在内皮素受体基因及蛋白表达;内皮素1具有明显促HEP2细胞增殖作用,且其促增殖作用呈浓度依赖性。结论内皮素1能有效促进HEP 2细胞有丝分裂,对喉癌的演进可能有重要调控作用。

 

关键词: 关键词:内皮素 1;HEP 2 细胞;有丝分裂

Abstract:

To investigate the effect of endothelin 1 on mitosis of HEP 2 cells. MethodsExpression of endothelin receptors on HEP 2 cells was determined by RTPCR and immunohistochemical methods. HEP 2 cell lines were exposed to different concentrations of the endothelin 1, and mitosis of HEP 2 cells was measured by 3ResultsGene expression of the endothelinreceptor was detected by RTPCR. Immunohistochemical studies showed that endothelin receptor immunoreactivity appeared in HEP 2 cells. Addition of endothelin 1 resulted in an increase of DNA synthesis in concentration dependence. ConclusionEndothelin 1 can promote mitosis of HEP 2 cells and may play an important role in the progression of laryngeal carcinoma.

 

Key words: Endothelin 1; HEP 2 cells; Mitosis

中图分类号: 

  • R76719
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