颈段食管癌患者外周血单个核细胞HLA-A和HLA-B mRNA的表达

山东大学耳鼻喉眼学报 ›› 2009, Vol. 23 ›› Issue (2): 8-11 .

颈段食管癌患者外周血单个核细胞HLA-A和HLA-B mRNA的表达

李浩¹，邹雄²，赵胜梅¹，张义²，李英杰¹，
陆楠¹，周玉侠¹，竺明晨¹，刘阳¹

（1. 山东大学医学院, 济南 250012；
2. 山东大学齐鲁医院检验科，济南 250012）

收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2009-04-16 发布日期:2009-04-16
通讯作者: 邹雄

Expressions of HLAA and B mRNA in peripheral blood mononuclear cells in patients with esophagus cancer of the neck

LI Hao¹, ZOU Xiong², ZHAO Shengmei¹, ZHANG Yi², LI Yingjie¹,
LU Nan¹,　ZHOU Yuxia¹,　ZHU Mingchen¹,　LIU Yang¹

(1. School of Medicine, Shandong University, Jinan 250012, China; 2.Department of Clinical Laboratory,
Qilu Hospital of Shandong University, Jinan 250012, China)

Received:1900-01-01 Revised:1900-01-01 Online:2009-04-16 Published:2009-04-16
Contact: ZOU Xiong

摘要/Abstract

摘要：

目的检测颈段食管癌患者外周血单个核细胞（peripheral blood mononuclear cell， PBMC）HLA-A和HLA-B mRNA的表达水平，探讨其在肿瘤免疫中的作用及其临床应用价值。方法收集32例颈段食管癌患者和45名健康体检者外周血单个核细胞，用实时荧光RTPCR方法检测淋巴细胞HLA-A和HLA-B mRNA的表达水平。结果PBMC的HLA-A和HLA-B mRNA在颈段食管肿瘤组的相对表达率分别为0.41±0.26和0.37±0.28，在正常对照组分别为1.12±0.54，1.19±0.60，颈段食管肿瘤组HLA-A和HLA-B mRNA相对表达率低于正常对照组（P均＜0.000?1)。多因素Logistic回归分析显示，HLA-A,B mRNA的表达与颈段食管癌的发生有关（P=0.01）。结论颈段食管癌患者外周血单个核细胞HLAA和HLAB mRNA表达水平低于正常对照组。HLA-A和HLA-B mRNA的表达水平或许可以作为一个新的肿瘤标志物，这对颈段食管癌的早期诊断和治疗提供了一个新的思路和方向。

关键词: 关键词：颈段食管癌；外周血单个核细胞；HLA；实时荧光定量PCR

Abstract:

To investigate expression levels of HLAA and B mRNA in peripheral blood mononuclear cells (PBMCs) in patients with esophagus cancer of the neck and to explore their clinical significance. MethodsPeripheral blood mononuclear cells HLAA and B mRNA were determined by a realtime reverse quantitative PCR method in 62 patients with esophagus cancer of the neck and 32 healthy individuals. ResultsThe relative expression ratio of PBMC HLAA and B mRNA was 0.41±0.26 and 0.37±0.28 in the carcinoma group and 1.12±0.54 and 1.19±0.60 in the control group. Expression levels of HLAA and B mRNA on PBMCs in esophagus cancer of the neck were significantly lower than that in the controls. Multiple logistic regression analysis showed that expression of HLAA,B was significantly associated with esophagus cancer of the neck(P=0.01). ConclusionHLAA and B mRNA play a critical role in the tumor immunity response. HLAA and B mRNA expression levels in PBMC are significantly reduced when tumors occur, so they may become available tumor markers.

Key words: Key words: Esophagus cancer of the neck; Peripheral blood mononuclear cell； HLA； Realtime quantitative PCR

中图分类号:

R735.1

李浩1 ，邹雄2 ，赵胜梅1 ，张义2 ，李英杰1 ，
陆楠1 ，周玉侠1 ，竺明晨1 ，刘阳1
. 颈段食管癌患者外周血单个核细胞HLA-A和HLA-B mRNA的表达[J]. 山东大学耳鼻喉眼学报, 2009, 23(2): 8-11 .

LI Hao1, ZOU Xiong2, ZHAO Shengmei1, ZHANG Yi2, LI Yingjie1, . Expressions of HLAA and B mRNA in peripheral blood mononuclear cells in patients with esophagus cancer of the neck[J]. JOURNAL OF SHANDONG UNIVERSITY (OTOLARYNGOLOGY AND OPHTHALMOLOGY), 2009, 23(2): 8-11 .

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