慢性咽炎患者咽分泌物中幽门螺杆菌的超高倍显微镜检测结果

山东大学耳鼻喉眼学报 ›› 2009, Vol. 23 ›› Issue (3): 60-62.

慢性咽炎患者咽分泌物中幽门螺杆菌的超高倍显微镜检测结果

李丽娜张延平刘金伟马磊张宗霖邓惠严罗晓培王亚英周凤书

1. 解放军总医院第二附属医院耳鼻喉科，北京 100091； 2. 北京康博众公司，北京 100086；3. 解放军316医院耳鼻喉科，北京 100091

收稿日期:2009-02-04 修回日期:2009-04-23 出版日期:2009-06-16 发布日期:2009-06-16
通讯作者: 张延平。
作者简介:李丽娜，女，主治医师，主要从事遗传性耳聋方面的研究。Email:lili7na@sina.com

Helicobacter pylori in throat swabs of patients with chronic pharyngitis under a multifunction microscopy

LI Li-Na, ZHANG Yan-Ping, LIU Jin-Wei, MA Lei, ZHANG Zong-Lin, DENG Hui-Yan, LUO Xiao-Pei, WANG Ya-Ying, ZHOU Feng-Shu

1. Department of Otolaryngology, Second Affiliated Hospital of General Hospital of Chinese PLA, Beijing 100091, China; 2. Beijing Kangbozhong Cooperation, Beijing 100086, China; 3. Department of Otolaryngology, No. 316 Hospital of Chinese PLA, Beijing 100091, China

Received:2009-02-04 Revised:2009-04-23 Online:2009-06-16 Published:2009-06-16

摘要/Abstract

摘要：

目的探讨超高倍显微镜检测慢性咽炎患者咽部分泌物中幽门螺杆菌(HP)的分布情况。方法对慢性咽炎患者295例和正常对照30例，采用咽拭子均匀涂片，超高倍显微镜下观察，放大20?000倍，根据观察需要选择暗视野相差视野进行活体观察，由一名专业人员进行结果判定。卡方检验评价两组HP阳性率的差异。结果慢性咽炎患者中229例咽部分泌物中检测到幽门螺旋杆菌（77.63%，229/295），66例未发现HP（22.37%，66/295）；正常对照组中, 30例咽部分泌物中2例检测到幽门螺旋杆菌（6.67%，2/30）,其余28例（93.33%，28/30）幽门螺旋杆菌阴性，经卡方检验χ2=6.670，P＜0.05。结论幽门螺旋菌可能是慢性咽炎患者咽部一种被忽视的致病菌，超高倍显微镜有可能成为筛查咽部分泌物中幽门螺旋菌的一种新方法，幽门螺旋菌感染与喉咽反流的关系还需要进行进一步研究证实。

关键词: 显微镜；幽门螺旋杆菌；咽炎

Abstract:

Objective To explore the helicobacter pylori（HP） distributions by multifunction microscopy in pharyngeal secretion of patients with chronic pharyngitis. Methods From September 2005 to December 2007, 295 patients with chronic pharyngitis and 30 controls were subjected to throat swab tests by multifunctional microscopy with 20000 magnification. Assessment was conducted by one professional worker. A Chi-square test was used to explore the statistical significance in terms of the outcome in the two groups. Results In the chronic pharyngitis group, HP was positive in 229 patients（77.63%，229/295）and negative in 66 ones（22.37%，66/295）. In the control group, HP was found in only 2 individuals（6.67%，2/30）and the rest were negative（93.33%，28/30）. The infective rate of HP was statistically significant between the chronic pharyntitis group and the control group (χ2=6.670，P＜0.05). Conclusions Helicobacter pylori might be a pathogen of chronic pharyngitis that has been neglected by physicians for a long time. Multifunction microscopy may provide a new method to screen HP in patients with chronic pharyngitis.

Key words: Microscopy; Helicobacter pylori; Paryngitis

中图分类号:

R766

李丽娜张延平刘金伟马磊张宗霖邓惠严罗晓培王亚英周凤书. 慢性咽炎患者咽分泌物中幽门螺杆菌的超高倍显微镜检测结果[J]. 山东大学耳鼻喉眼学报, 2009, 23(3): 60-62.

LI Li-Na, ZHANG Yan-Ping, LIU Jin-Wei, MA Lei, ZHANG Zong-Lin, DENG Hui-Yan, LUO Xiao-Pei, WANG Ya-Ying, ZHOU Feng-Shu. Helicobacter pylori in throat swabs of patients with chronic pharyngitis under a multifunction microscopy[J]. JOURNAL OF SHANDONG UNIVERSITY (OTOLARYNGOLOGY AND OPHTHALMOLOGY), 2009, 23(3): 60-62.

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