声强对豚鼠下丘给声响应神经元间隔探测的影响

山东大学耳鼻喉眼学报 ›› 2009, Vol. 23 ›› Issue (4): 41-44.

声强对豚鼠下丘给声响应神经元间隔探测的影响

苏开明陈正侬关建于栋祯时海波殷善开

上海交通大学医学院附属第六人民医院耳鼻咽喉科，上海 200233

收稿日期:2009-03-19 修回日期:2009-08-02 出版日期:2009-08-16 发布日期:2009-08-16
作者简介:苏开明（1975- ），男，医学博士，主要从鼻科、耳科的基础与临床研究。 Email:021china@sina.com
基金资助:
上海交通大学医学院科技基金（09XJ21004）

Effects of stimulus intensity on gap detection in the onset unit of the inferior colliculus in guinea pigs

SU Kai-Meng, CHEN Zheng-Nong, GUAN Jian, XU Dong-Zhen, SHI Hai-Bei, YAN Shan-Kai

Department of Otolaryngology, Affiliated Shanghai Sixth People's Hospital of Shanghai Jiao Tong University of Medicine, Shanghai 200233, China

Received:2009-03-19 Revised:2009-08-02 Online:2009-08-16 Published:2009-08-16

摘要/Abstract

摘要：

目的探讨声音信号强度对豚鼠下丘给声响应神经元间隔探测的影响。方法采用单细胞记录的方法，刺激信号由100ms和50ms白噪声相加组成，声音强度10～80dB SPL，中间间隔时间为0、1、2、4、8、16、48、96?ms，分别统计阈响应组和阈上10，20，30dB时，给声响应神经元的间隔阈值，并观察间隔阈值与放电率和潜伏期的关系。结果本实验共记录96个神经元，其中，给声响应神经元有35个，在阈响应强度时，给声响应神经元间隔阈值明显增大，四组强度的间隔阈值均值分别为(22.91±4.36)ms，(9.00±2.69)ms，(4.00±0.49)ms和(11.33±3.11)ms（P<0.01），但阈上各组间的差异无统计学意义（P>0.05）。在间隔时间为48ms时，给声响应神经元间隔后放电恢复率的均值分别为0.44±0.15，0.83±0.12，0.88±0.07和0.61±0.10（P<0.01）。而首次放电潜伏期分别为(12.86±0.72)ms，(11.65±0.64)ms，(11.03±0.65)ms和(10.68±0.55)ms（P>0.05）。结论声音信号强度对豚鼠下丘给声响应神经元的间隔探测有一定影响。

关键词: 豚鼠；下丘；间隔探测；刺激强度

Abstract:

Objective To explore the effects of stimulus intensity on gap detection responses of onset neurons in inferior colliculus(IC) of guinea pigs. Methods Gap stimuli were noise bursts, with levels from 10 to 80?dB SPL. A series of silent gaps including 0, 1, 2, 4, 8, 16, 48 and 96ms gap duration were embedded between the initial 100?ms white noise burst (NB1) and the final 50ms burst (NB2) in the stimuli. The minimal gap threshold (MGT), first spike latency (FSL) andNB2 recovery rate were also analyzed according to four intensity groups: minimum threshold (MT), MT+10dB SPL (MT+10), MT+20dB SPL(MT+20), and MT+30dB SPL(MT+30). ResultsThirtyfive onset units were collected from 96 recorded neurons. The average MGT was (22.91±4.36)ms, (9.00±2.69)ms, (4.00±0.49)ms and (11.33±3.11)ms in the groups of MT, MT+10, MT+20 and MT+30, respectively (P<0.01). However, this effect was not significant among the group of suprathreshold. The NB2recovery rate in 48ms gap duration was 0.44±0.15, 0.83±0.12, 0.88±0.07 and 0.61±0.10, respectively. The FSL in 0ms gap duration was (12.86±0.72)ms, (11.65±0.64)ms, (11.03±0.65)ms and (10.68±0.55)ms respectively in the four groups. ConclusionThe level of white noise gap stimuli has some effects on thegap response in the onset unit of inferior colliculus in guinea pigs.

Key words: Guinea pig; Inferior colliculus; Gap detection; Stimulus intensity

中图分类号:

R764.4

苏开明陈正侬关建于栋祯时海波殷善开. 声强对豚鼠下丘给声响应神经元间隔探测的影响[J]. 山东大学耳鼻喉眼学报, 2009, 23(4): 41-44.

SU Kai-Meng, CHEN Zheng-Nong, GUAN Jian, XU Dong-Zhen, SHI Hai-Bei, YAN Shan-Kai. Effects of stimulus intensity on gap detection in the onset unit of the inferior colliculus in guinea pigs[J]. JOURNAL OF SHANDONG UNIVERSITY (OTOLARYNGOLOGY AND OPHTHALMOLOGY), 2009, 23(4): 41-44.

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