生后大鼠下丘GABAA受体β2和γ2亚单位的表达

山东大学耳鼻喉眼学报 ›› 2010, Vol. 24 ›› Issue (01): 25-28.

生后大鼠下丘GABAA受体β2和γ2亚单位的表达

张媛媛1，刘得龙2，张庆丰2，陶然1

1. 大连大学医学院，辽宁大连 116622； 2. 大连市中心医院耳鼻咽喉科，辽宁大连 116033

收稿日期:2009-05-05 修回日期:2009-12-05 出版日期:2010-02-16 发布日期:2010-02-16
通讯作者: 刘得龙
作者简介:张媛媛，女，医学博士、博士后，讲师，主要从事细胞生物学与分子生物学方面的研究。 E-mail: yyzh78@yahoo.com.cn

Expressions of GABAA receptor subunits in the inferior colliculus of newborn rats

ZHANG Yuanyuan1, LIU Delong2, ZHANG Qingfeng2，　TAO Ran1

1. Medical College of Dalian University, Dalian 116622, Liaoning, China;
2. Department of Otolaryngology, Dalian Central Hospital, Dalian 116033, Liaoning, China

Received:2009-05-05 Revised:2009-12-05 Online:2010-02-16 Published:2010-02-16

摘要/Abstract

摘要：

目的研究大鼠发育期下丘GABAAR β2和γ2亚单位的表达规律。方法选用生后P1到P16天的正常大鼠，将下丘组织提取的GABAARβ2和γ2亚单位mRNA用RT-PCR半定量，原位杂交方法定位和定性。将引物与内参GAPDH一起进行PCR，产物的电泳结果在紫外分析系统下进行灰度分析比较。用非放射性寡核苷酸探针进行原位杂交。结果 GABAARβ2和γ2亚单位在生后正常大鼠下丘内表达的总体趋势是非线性增加的，P7表达最低，P13为高峰，P16下降到与成年时的水平相当。差异有统计学意义（P<0.01）。下丘GABAARβ2和γ2亚单位的表达模式相似。结论 GABAAR两种亚单位在生后大鼠下丘的表达是阶段性和波动性的。两种亚单位在下丘内同步表达。

关键词: γ-氨基丁酸A受体；β2亚单位；γ2亚单位；下丘；大鼠

Abstract:

Objective To explore expressions of GABAAR β2 and γ2 subunits in the inferior colliculus of newborn rats. Methods mRNAs expressions of GABAAR β2 and γ2 subunits in the inferior colliculus of newborn rats from 1 to 16 days were quantitated by half quantitation PT-PCR and located by in situ hybridization. The PCR were done with interior control GAPDH. The gray scales of the electrophoresis products of PCR were observed and analyzed under an ultraviolet analytical system. In situ hybridization was done by a non-radioactive oligonucleotide probe and the results were observed by a light microscope. Results Expressions of GABAAR β2 and γ2 subunits in the inferior colliculus were gradually increased in a nonlinear way. Expression level was the lowest at day 7, reached a peak at day 13 and dropped. to a level similar with that of adult rats at day 16. The differences were significant（P<0.01）. Conclusion Expressions of GABAAR β2 and γ2 subunits in the inferior colliculus were undulatory and phasic in postnatal rats. The two subunits were co-expressed in the inferior colliculus.

Key words: γ-aminobutyricacid type A receptor; β2 subunit; γ2 subunit; Inferior Colliculus; Rats

中图分类号:

R764.4

张媛媛1，刘得龙2，张庆丰2，陶然1. 生后大鼠下丘GABAA受体β2和γ2亚单位的表达[J]. 山东大学耳鼻喉眼学报, 2010, 24(01): 25-28.

ZHANG Yuanyuan1, LIU Delong2, ZHANG Qingfeng2，　TAO Ran1. Expressions of GABAA receptor subunits in the inferior colliculus of newborn rats[J]. JOURNAL OF SHANDONG UNIVERSITY (OTOLARYNGOLOGY AND OPHTHALMOLOGY), 2010, 24(01): 25-28.

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