山东大学耳鼻喉眼学报 ›› 2010, Vol. 24 ›› Issue (5): 36-39.

• 论文 • 上一篇    下一篇

大鼠嗅感觉上皮发育及嗅细胞凋亡的研究

史保院1, 史丽2,马超2   

  1. 1.  河南大学淮河医院耳鼻咽喉科,  河南 开封 475000;2. 山东大学齐鲁医院耳鼻咽喉-头颈外科,  济南 250012
  • 收稿日期:2010-04-28 修回日期:2010-09-22 出版日期:2010-10-16 发布日期:2010-10-16
  • 通讯作者: 史丽,女,教授,博士生导师。 Email: shili126@sina.com
  • 作者简介:史保院, 男,硕士,主治医师, 主要从事耳鼻喉科疾病的临床诊治。 Email: shiby74@163.com

Investigation of the development and apoptosis of  the mouse olfactory epithelium

SHI Bao-yuan1,  SHI Li2,  MA Chao2   

  1. 1. Department of Otolaryngology,Huaihe Hospital of Henan University, Kaifeng 475000,  Henan, China;
    2. Department of OtolaryngologyHead and Neck Surgery, Qilu Hospital of Shandong University, Jinan 250012, China
  • Received:2010-04-28 Revised:2010-09-22 Online:2010-10-16 Published:2010-10-16

摘要:

目的      探讨神经特异性烯醇酶(NSE)、嗅细胞标记蛋白(OMP)及细胞凋亡在不同胎龄大鼠嗅黏膜中的表达。方法     免疫组化方法检测NSE及OMP在不同孕期大鼠嗅上皮中的表达及其规律。TUNEL方法检测不同孕期大鼠嗅上皮中凋亡细胞并计算细胞凋亡指数(AI)。结果     E13d鼻腔黏膜中即有NSE阳性表达,细胞数量多。E13d嗅上皮中未见OMP阳性表达细胞。在E14d,嗅上皮中出现嗅OMP阳性细胞,数量少,随胎龄增加阳性细胞数量增多,至17d达高峰并逐渐趋于稳定。E13~E15d细胞凋亡数量较稳定,至E16d凋亡细胞数量明显增加,达到高峰,E18~E21d凋亡细胞数量逐渐减少渐趋于稳定。E16dAI与其他d数差异有统计学意义。结论        E14d嗅黏膜中已有发育成熟的嗅细胞,数量少,胚胎发育后期大鼠嗅化学感受器发育已趋于成熟。在嗅上皮发育过程中存在细胞凋亡,E16d嗅细胞凋亡出现一高峰,细胞凋亡在嗅觉发育过程中起重要作用。

关键词: 嗅上皮;神经特异性烯醇酶;嗅细胞标记蛋白;细胞凋亡小体;凋亡指数

Abstract:

Objective     To study the expression of neuro-specific enolase(NSE),  ofactory marker protein(OMP)and  apoptosis in the development of olfactory mucosa of mouse fetuses. Methods      Expressions of NSE and OMP in the olfactory mucosa of the different embryonic stages(day 13 to 21)were determined using immunohistochemical technique. The Tdt-mediated dUTP nick end-labeling technique (TUNEL) was used to detect apoptotic cells of the olfactory epithelium in different embryonic stages and to caculate the apoplotic index. Results       NSE immunological positive reactions were seen in different embryonic stages from E13 to E21. Otherwise, few positive OMP cells were observed after E14,  after E15  OMP-positive neurons were noticeably increased in the olfactory epithelium and at E17, they reached the peak. The apoptotic process in the olfactory epithelium was observed form E13 to E21. At E13, the apoptotic cells were few and the AI was low. A massive wave of cell death was evidenced between E16 and E18.The apoptotic index of E16 was noticeably distinction from other embryonic stages. Conclusion      Olfactory receptor neurons of mouse fetuses became mature at E14 and the quantity was small. In the anaphase of the embryo, the olfactory epithelium was mature. The apoptotic process takes part in the development of the mouse olfactory epithelium and comes to a peak in E16. Apoptosis is an active process in the development of mouse olfactory epithelium.

Key words: Olfactory epithelium; Neurospecial endolase; Olfactory marker protein; Apoptotic body; Apoptotic index

中图分类号: 

  • R765.6
[1] 黄珏,方芳,章左艳. 慢性鼻窦炎伴弥漫性泛细支气管炎1例[J]. 山东大学耳鼻喉眼学报, 2012, 26(4): 91-92.
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