FK506拮抗庆大霉素耳毒性的实验观察

山东大学耳鼻喉眼学报 ›› 2010, Vol. 24 ›› Issue (6): 13-16.

FK506拮抗庆大霉素耳毒性的实验观察

王晓1，鞠宏霜2，梁桂玲1，闫永红1，苏梅1

1. 山东大学齐鲁医院耳鼻咽喉科，济南 250012；2. 山东大学齐鲁医院桓台分院耳鼻咽喉科，山东桓台 256400

收稿日期:2010-05-02 修回日期:2010-07-12 发布日期:2010-12-16
通讯作者: 鞠宏霜。Email: 13583300844@163.com
作者简介:王晓（1979- ），女，主要从事耳鼻咽喉专业临床工作。Email: zkzkzkwxwxwx@yahoo.com.cn

FK506 as antagonist to ototoxicity of Gentamycin

WANG Xiao1, JU Hong-shuang2, LIANG Gui-ling1, YAN Yong-hong1, SU Mei1

1. Department of Otolaryngology, Qilu Hospital of Shandong University, Jinan 250012, China;
2. Department of Otolaryngology, Huantai County, Qilu Hospital of Shandong University, Huantai 256400, Shandong, China

Received:2010-05-02 Revised:2010-07-12 Published:2010-12-16

摘要/Abstract

摘要：

目的初步探讨FK506拮抗庆大霉素耳毒性的效果。方法通过分别检测庆大霉素损伤组与庆大霉素加不同剂量FK506（1mg、2mg）保护组实验动物听性脑干反应(ABR)阈值的变化，并结合耳蜗铺片琥珀酸脱氢酶（SDH)染色方法观察动物耳蜗形态学变化，观察FK506拮抗庆大霉素耳毒性的作用。结果实验动物的听觉生理检查显示FK506能够拮抗庆大霉素耳毒性,FK506保护组动物听阈明显低于庆大霉素损伤组(P<0.01)，随剂量增加，保护作用越明显；同时形态结果显示FK506保护组外毛细胞缺失明显少于庆大霉素损伤组(P<0.01)。结论 FK506对庆大霉素引起的耳毒性有拮抗作用。

关键词: 耳毒性;FK506;庆大霉素

Abstract:

Objective To investigate the effects of FK506 to antagonize ototoxicity of Gentamycin. Methods The gentamycin group and the groups of gentamycin plus different doses of FK506 (1mg, 2mg) were set up. Then, the auditory brainstem response (ABR) threshold was measured to explore the changes in hearing function. Cochlear preparations were stained with SDH to observe morphological changes. Results Auditory data and morphological changes demonstrated significant differences between the gentamycin group and the groups of FK506 (P<0.05). FK506 could reduce the damages of gentamycin in the cochlea. Conclusion Based on the experiment, FK506 can relieve the ototoxicity induced by gentamycin.

Key words: Ototoxity; FK506; Gentamycin

中图分类号:

R978.12

王晓1，鞠宏霜2，梁桂玲1，闫永红1，苏梅1. FK506拮抗庆大霉素耳毒性的实验观察[J]. 山东大学耳鼻喉眼学报, 2010, 24(6): 13-16.

WANG Xiao1, JU Hong-shuang2, LIANG Gui-ling1, YAN Yong-hong1, SU Mei1. FK506 as antagonist to ototoxicity of Gentamycin[J]. JOURNAL OF SHANDONG UNIVERSITY (OTOLARYNGOLOGY AND OPHTHALMOLOGY), 2010, 24(6): 13-16.

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