Notch-1、NF-kB和β-catenin在口腔鳞状细胞癌中的表达及其临床意义

山东大学耳鼻喉眼学报 ›› 2011, Vol. 25 ›› Issue (1): 9-13.

Notch-1、NF-kB和β-catenin在口腔鳞状细胞癌中的表达及其临床意义

李雪珍1，金晓明1，刘民生2

1. 青岛大学医学院口腔颌面外科，山东青岛 266011； 2. 莱芜市中医医院耳鼻喉科，山东莱芜 271100

收稿日期:2010-09-01 修回日期:2010-09-28 出版日期:2011-02-16 发布日期:2011-02-16
通讯作者: 金晓明。Email:jinxmqyfy@yahoo.com.cn
作者简介:李雪珍（1976- ）,女，硕士研究生，主要从事口腔疾病的临床诊治研究。 Email:sdlwlxz@163.com

Expression and clinicopathological significance of Notch-1，NF-kB and β-catenin in oral squamous cell carcinoma

LI Xue-zhen1， JIN Xiao-ming1， LIU Min-sheng2

1．Department of Oral and Maxillofacial Surgery, Medical College of Qingdao University,
Qingdao 266011, Shandong, China;
2．Department of Otolaryngology, Chinese Medicine Hospital of Laiwu, Laiwu 271100, Shandong, China

Received:2010-09-01 Revised:2010-09-28 Online:2011-02-16 Published:2011-02-16

摘要/Abstract

摘要：

目的通过观察Notch-1、NF-kB和β-catenin在口腔鳞状细胞癌中的表达情况，探讨Notch-1、NF-kB及Wnt/1β-catenin信号传导系统与口腔鳞状细胞癌发生、发展的相关性。方法应用免疫组织化学染色方法检测58例口腔鳞状细胞癌组织及30例癌周组织中Notch-1、NF-kB和β-catenin蛋白表达情况并分析其与临床病理特征的关系。结果 ①Notch-1、NF-kB蛋白在口腔鳞状细胞癌组织中的表达明显高于在癌周组织，并且两者染色积分间的差异有统计学意义(P<0.01)。两种蛋白的表达水平与患者的年龄、性别、肿瘤原发部位和组织学分级之间无相关性，但是与肿瘤TNM分期以及有无淋巴结转移密切相关(P<0.01)；②在58例口腔鳞状细胞癌组织中β-catenin异位表达23例(79.31％)，β-catenin异位表达与肿瘤TNM分期(P<0.01)以及有无淋巴结转移密切相关(P<0.05)；③Spearman等级相关分析口腔鳞状细胞癌组织中Notch-1、NF-kB表达和β-catenin异位表达之间的相关性，Notch-1和NFkB表达二者表达有显著关联性 (P<0.05)；Notch-1表达和β-catenin异位表达差异有统计学意义(P<0.05)；而NFkB蛋白表达和βcatenin异位表达未见明显相关性 (P>0.05)。结论 Notch-1、NF-ΚB高表达和β-catenin异位表达在口腔鳞状细胞癌中属常见现象，并且三者表达情况均与肿瘤TNM分期以及有无淋巴结转移密切相关。Notch-1表达分别和NF-kB表达及β-catenin异位表达差异有统计学意义，但NF-kB表达和β-catenin异位表达差异无统计学意义。

关键词: 口腔鳞状细胞癌；Notch-1；NF-ΚB；β-catenin；表达

Abstract:

Objective To investigate expressions of Notch-1，NF-kB and β-catenin in oral squamous cell carcinoma, and explore their associations with the clinicopathological features. Methods Expressions of Notch-1，NF-kB and β-catenin were analyzed in 58 oral squamous cell carcinoma samples and 30 normal tissues adjacent to the carcinomas by immunohistochemistry. Results a) Notch-1 and NF-kB protein were up-regulated in most oral squamous cell carcinoma samples but not in normal tissues. The difference of weighted scores between these two groups was significant (P<0.01). Although there were no correlations between the expressions of Notch1，NFkB protein and some tumor parameters, such as age, gender, tumor site and histological grade, expressions of Notch-1，NF-kB protein were significantly associated with clinical staging (P<0.01) and lymph node metastasis (P<0.05). b) Higher abnormalβ-catenin expression was noted in most oral squamous cell carcinoma samples than in normal tissues. The difference of weighted scores between these two group was also significant (P<0.01). There were no correlations between expressions of abnormalβcatenin expression and some tumor parameters, such as age, gender, tumor site and histological grade, while expression of abnormalβ-catenin was significantly associated with clinical staging (P<0.01) and lymph node metastasis (P<0.05). c) In oral squamous carcinoma tissues, there were correlation between expression of Notch-1 and NF-kB, and there were also correlation between expression of Notch-1 and ectopic expression of β-catenin (P<0.05)． But there was no correlation between expression of NF-kB and ectopic expression of β-catenin (P>0.05). Conclusions Over-expression of Notch-1， NF-kB or ectopic expression of β-catenin expression are common in oral squamous cell carcinoma. Expression of Notch-1 and NF-kB or ectopic expression of β-catenin expression are significantly correlated with tumor staging and lymph node metastasis. Expression of Notch-1 were correlatied with expression of NF-kB and ectopic expression of β-catenin, but there was no correlation between expression of NF-kB and ectopic expression of β-catenin.

Key words: Oral squamous cell carcinoma； Notch-1；NF-kB；β-catenin; Expression

中图分类号:

R739.8

李雪珍1，金晓明1，刘民生2. Notch-1、NF-kB和β-catenin在口腔鳞状细胞癌中的表达及其临床意义[J]. 山东大学耳鼻喉眼学报, 2011, 25(1): 9-13.

LI Xue-zhen1， JIN Xiao-ming1， LIU Min-sheng2. Expression and clinicopathological significance of Notch-1，NF-kB and β-catenin in oral squamous cell carcinoma[J]. JOURNAL OF SHANDONG UNIVERSITY (OTOLARYNGOLOGY AND OPHTHALMOLOGY), 2011, 25(1): 9-13.

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