急性分泌性中耳炎调节性T细胞及T淋巴细胞亚群的分析

山东大学耳鼻喉眼学报 ›› 2012, Vol. 26 ›› Issue (1): 26-28.

急性分泌性中耳炎调节性T细胞及T淋巴细胞亚群的分析

刘振1，王跃健1，肖平2

佛山市第一人民医院 1.耳鼻咽喉科； 2.临床研究所，广东佛山 528000

收稿日期:2011-07-15 出版日期:2012-02-16 发布日期:2012-02-16
作者简介:刘振，副主任医师。 Email:liuzzz1@126.com
基金资助:
广东省医学科研基金资助(No:A2009654)。

Tregs and lymphocyte subpopulations in the acute secretory otitis media patients.

LIU Zhen1, WANG Yue-Jian1, XIAO Ping2. 1

Department of Otolaryngology & Head and Neck Surgery, 2. Clinic Institute, First People′s Hospital of Foshan, 528000, Guangdong.

Received:2011-07-15 Online:2012-02-16 Published:2012-02-16

摘要/Abstract

摘要：

目的检测急性分泌性中耳炎(SOM)患者外周血调节性T细胞及淋巴细胞亚群的变化，综合分析免疫因素在其发病中的作用。方法以流式细胞术检测38例上感后诱发急性SOM患者，30例健康献血者外周血中CD3+、CD4+、CD8+、NK细胞、B淋巴细胞百分比，CD4+/CD8+比值，CD4+CD25+调节性T细胞百分比以及T淋巴细胞亚群的计数。结果患者组CD3+CD8+、NK细胞的百分比明显低于正常对照组(P<0.05)；CD4+/CD8+、B淋巴细胞比例明显高于正常对照组(P<0.05)；正常对照组Tregs占CD4+T淋巴细胞的比例为(6.695±1.09)%，患者组占CD4+T淋巴细胞的比例为(12.82±4.56)%，与正常对照组比较差异有统计学意义(P<0.05)；两组间CD3+、CD4+细胞比例，差异无统计学意义(P>0.05)；正常对照组及患者组外周血CD3+、CD3+CD4+、CD3+CD8+T淋巴细胞计数，两组之间差异无显著性(P>0.05)。结论急性分泌性中耳炎患者存在着免疫失调，CD4+/CD8+下降，Tregs比例异常增高，说明免疫异常在SOM的发病中起一定的作用。

关键词: 分泌性中耳炎；流式细胞术；淋巴细胞

Abstract:

Objective To investigate the changes of Tregs and lymphocyte subpopulations in the peripheral blood of the patients with SOM (secretory otitis media), and explore the influence of immunological status on the development of SOM. Methods Peripheral blood samples of thirtynine patients and thirty healthy donors were prepared. The percent of CD4+CD25+、CD3+、CD4+、CD8+、NK、B lymphocyte and the ratio of CD4+/CD8+ were analyzed by tricolour flow cytometry. Results The percentage of CD3+CD8+、NK in the patients group was lower than that of the control group(P<0.05)；The percentage of CD4+/CD8+、B lymphocyte was higher than that of the control group. The percentage of Tregs in the patient and control group were (12.82±4.56)% and (6.695±1.09)%, respectively(P<0.05). There were no significant differences in the number of CD3+、CD3+CD4+、CD3+CD8+T lymphocyte and the percentage of CD3+、CD4+ T lymphocyte. Conclusion The ratio of CD4+/CD8+ in the SOM patients is significantly lower than that of the healthy and the ratio of Tregs is higher, which implies that immune imbalance existes in the acute secretory otitis media patients.

Key words: Secretory otitis media； Flow eytometry； Lymphocyte

中图分类号:

R725.6

刘振1，王跃健1，肖平2. 急性分泌性中耳炎调节性T细胞及T淋巴细胞亚群的分析[J]. 山东大学耳鼻喉眼学报, 2012, 26(1): 26-28.

LIU Zhen1, WANG Yue-Jian1, XIAO Ping2. 1. Tregs and lymphocyte subpopulations in the acute secretory otitis media patients.[J]. JOURNAL OF SHANDONG UNIVERSITY (OTOLARYNGOLOGY AND OPHTHALMOLOGY), 2012, 26(1): 26-28.

参考文献

多维度评价

Viewed

Full text

Abstract

Cited

Shared

Discussed