高迁移蛋白A1与埃兹蛋白在喉癌组织中的表达及相关性研究

山东大学耳鼻喉眼学报 ›› 2012, Vol. 26 ›› Issue (2): 27-31.

高迁移蛋白A1与埃兹蛋白在喉癌组织中的表达及相关性研究

潘春晨1,张小鹏2，赖海春3，王德生3

1.福建医科大学协和临床医学院，福州 350001；　2.暨南大学附属第一医院妇产科，广州 510632；
　3.福建医科大学附属协和医院耳鼻咽喉头颈外科，福州 350001

收稿日期:2011-10-07 出版日期:2012-04-16 发布日期:2012-04-16
通讯作者: 王德生，硕士生导师。Email:fzwds@yahoo.com
作者简介:潘春晨，硕士研究生。Email:pccfz1985@gmail.com
基金资助:
福建省自然科学基金资助项目（2011J01174）

Expression and relationship of HMGA1 and Ezrin in laryngeal carcinoma

PAN Chun-chen1, ZHANG Xiao-peng2, LAI Hai-chun3, WANG De-sheng3

1. Xiehe Clinic Medicine College of Fujian Medical University, Fuzhou 350001, China; 2. Department of Gynecology and Obstetrics, The Affiliated First Hospital of Jinan University, Guangzhou 510630, China; 3. Department of Otorhinolaryngology & Head and Neck Surgery, The Affiliate Union Hospital of Fujian Medical University, Fuzhou 350001, China

Received:2011-10-07 Online:2012-04-16 Published:2012-04-16

摘要/Abstract

摘要：

目的探讨高迁移蛋白A1（HMGA1）与埃兹蛋白（Ezrin）在喉癌组织中的表达及关系。方法采用免疫组织化学染色方法检测HMGA1、Ezrin蛋白在50例喉癌组织、30例喉不典型增生组织、30例喉息肉中的表达；采用实时定量PCR法检测HMGA1、Ezrin mRNA在30例喉癌组织及30例癌切缘组织的表达，统计分析二者表达与临床病理特征的相关性。结果 HMGA1和Ezrin蛋白、mRNA在喉癌组织中的阳性表达率均高于对照组(P<0. 05)， HMGA1和Ezrin蛋白、mRNA表达与喉癌组织的临床分期(P<0.05)、组织学分级(P<0.05)及转移(P<0.05)相关,而与患者年龄、性别、吸烟差异无统计学意义(P>0.05)。喉癌组织中HMGA1及Ezrin蛋白、mRNA水平表达正相关(实时定量PCR，r=0.385，P=0.000；免疫组化，χ2=7.172，r=0.379，P=0.012)。结论 HMGA1与Ezrin在喉癌的侵袭、转移过程中发挥调控作用，可为喉癌的早期诊断和治疗提供新的分子指标。

关键词: 喉肿瘤；高迁移蛋白A1；埃兹蛋白；侵袭；转移

Abstract:

Objective To investigate expression of HMGA1 and Ezrin in laryngeal carcinoma and analyze their clinical significance and relationship. Methods Expression of proteins of HMGA1 and Ezrin was semi-quantitatively analyzed by SP immunohistochemical staining in 50 cases of laryngeal carcinoma. 30 cases of laryngeal polyp and 30 cases of atypical laryngeal hyperplasia were studied as controls. Expressions of mRNA of HMGA1 and Ezrin were analyzed by rea-time PCR in 30 cases of laryngeal carcinoma. Also, 30 cases of cancer cutting-margin were studied as controls. Results (1) The positive rates of HMGA1 protein were 68.0%(34/50), 53.0%(16/30) and 13.0%(4/30) in laryngeal carcinoma, atypical hyperplasia of laryngea and laryngeal polyps, while Ezrin protein were 64.0% (32/50), 50.0%(15/30) and 23.3%(7/30), respectively. (2) Expression of HMGA1 and Ezrin proteins and mRNA was closely related to clinical stage, pathological stage and lymph node metastasis (P<0.05), but not patients′age, gender or tumor location(P>0.05). There was a positive correlation between the protein and mRNA expression of HMGA1 and Ezrin. (r=0.385,P=0.000, by real time PCR；χ2 =7.172，r=0.379，P=0.012, by IHC). Conclusion Over-expression of HMGA1 and Ezrin may contribute to the carcinogenesis, development and metastasis of laryngeal carcinoma. They may be new markersto evaluate the prognosis of laryngeal carcinoma.

Key words: Laryngeal carcinoma; High mobility group protein A1; Ezrin; Invasion; Metastasis

中图分类号:

R739.6

潘春晨1,张小鹏2，赖海春3，王德生3. 高迁移蛋白A1与埃兹蛋白在喉癌组织中的表达及相关性研究[J]. 山东大学耳鼻喉眼学报, 2012, 26(2): 27-31.

PAN Chun-chen1, ZHANG Xiao-peng2, LAI Hai-chun3, WANG De-sheng3. Expression and relationship of HMGA1 and Ezrin in laryngeal carcinoma[J]. JOURNAL OF SHANDONG UNIVERSITY (OTOLARYNGOLOGY AND OPHTHALMOLOGY), 2012, 26(2): 27-31.

参考文献

相关文章 14

[1]	赵苗青1，夏明2，林晓燕1，刘莹1，许风雷2，覃业军1. 颈段食管癌组织中FOXP3蛋白表达、肿瘤间质浸润FOXP3+淋巴细胞数量的临床意义[J]. 山东大学耳鼻喉眼学报, 2012, 26(6): 20-22.
[2]	王洪明1，薛维爽2，何睿妍3，姜学钧1. 局部注射平阳霉素与CO2激光治疗咽喉部血管瘤疗效比较[J]. 山东大学耳鼻喉眼学报, 2012, 26(6): 26-27.
[3]	沈斌，董频，英信江，高尚，李大伟. ABCG2及NF-κB p65在下咽癌中的表达意义[J]. 山东大学耳鼻喉眼学报, 2012, 26(4): 17-20.
[4]	成伟1，陈健智2. 原发性扁桃体恶性肿瘤误诊、诊断及鉴别诊断探讨[J]. 山东大学耳鼻喉眼学报, 2012, 26(3): 61-62.
[5]	王军，马莉，高刚，杨菊芳. 二氧化碳激光治疗会厌囊肿42例[J]. 山东大学耳鼻喉眼学报, 2012, 26(1): 46-47.
[6]	张静，江文霞，马玲国，王木清，刘苑美. 颞下窝肿瘤切除术患者的围手术期护理[J]. 山东大学耳鼻喉眼学报, 2012, 26(1): 73-75.
[7]	李俐华，任基浩, 殷团芳，刘伟. 儿童复发性呼吸道乳头状瘤组织中STAT3、VEGF的表达及微血管密度测定与复发、侵袭的关系[J]. 山东大学耳鼻喉眼学报, 2011, 25(6): 11-.
[8]	魏东敏，雷大鹏，金童，刘大昱，许风雷，潘新良. 舌根鳞状细胞癌37例临床分析[J]. 山东大学耳鼻喉眼学报, 2011, 25(6): 23-.
[9]	孙峰1综述,孙彦2审校. 喉癌患者手术后生存质量的变化研究现状[J]. 山东大学耳鼻喉眼学报, 2011, 25(6): 26-29.
[10]	陈金辉，陶泽璋. 端粒酶逆转录酶和细胞增殖核抗原在喉鳞癌组织及细胞中的表达意义[J]. 山东大学耳鼻喉眼学报, 2011, 25(3): 5-8.
[11]	刘蓉蓉，姬长友，陈继川. 重组人p53腺病毒注射液（今又生）联合放疗治疗复发性鼻咽癌疗效观察[J]. 山东大学耳鼻喉眼学报, 2010, 24(5): 13-16.
[12]	刘章，孙彦，华辉，陈志俊，李薇，孙文海. 山东东部地区女性喉癌患者的临床特点[J]. 山东大学耳鼻喉眼学报, 2010, 24(2): 8-10.
[13]	李军政，江东新，陈伟南，焦文成，高翔，黄佩仪，梁悦浓，陈文弦. 鼻内镜下咽鼓管置管治疗鼻咽癌放疗后分泌性中耳炎[J]. 山东大学耳鼻喉眼学报, 2009, 23(3): 15-16.
[14]	叶萍，孙睿杰，金童，钱晔，魏东敏，潘新良. Caveolin-1在下咽鳞癌中的表达及其临床意义[J]. 山东大学耳鼻喉眼学报, 2012, 26(5): 22-24.

多维度评价

Viewed

Full text

Abstract

Cited

Shared

Discussed