关键词: 甲状腺乳头状癌；单核苷酸基因多态性；转化生长因子-β1

Abstract:

Objective   To observe single nucleotide polymorphisms of transforming growth factor-β1(TGF-β1) G-800A and C-509T, and to analyze the relationship between different types of their polymorphisms and susceptibility of papillary thyroid carcinoma (PTC) in Han adults in Jinan city. Methods   Distribution of alleles of TGF-β1 G-800A and C-509T SNP were analyzed by polymerase chain reaction-restriction fragment length polymorphisms (PCR-RFLP). The linkage disequilibrium relationships were observed between susceptibility of the PTC and TGF-β1 G-800A and C-509T polymorphisms in a case-control study. Results   TGF β-1 C-509 T and G-800 A linkage equilibrium factors are D′= 0.75, r2=0.83, P=0.03; In a population of Han located in Jinan city: adjusted by wild type GC, number of T GF β-1 GT haploid type, in both PTC patients and control, OR was 2.206(P<0.0001), this demonstrated that GT haploid type strengthened PTC susceptibility; The TGF β-1 G/G + C/C genotype，adjusted by non-mutated TGF β-1 G/G + C/C in both PTC patients and control, OR was 2.437(P=0.010). This demonstrated that the TGF β-1 G/A+T/T genotype enhanced PTC susceptibility; TGF β-1 C-509 T T/T was related to the wild type  C/C, OR=3.826, P<0.0001， that demonstrated that the TGF β-1 C-509 T T/T haploid type strengthened PTC susceptibility. Conclusion   In the Jinan Han ethnic group, the TGF-β 1 haploid type GT and merged genotype GA+TT increased PTC susceptibility. Also, the TGF β1 C509 T T/T genotype significantly increased susceptibility to PTC. TGF-β1 in G-800A, and C-509T chain single nucleotide polymorphisms can be used as markers for individual monitoring. TGFβ1 C-509T was closely related to high risk of PTC and might be a therapeutic target in PTC.

Key words: Papillary thyroid carcinoma; Single nucleotide polymorphism； Transforming growth factor-β1