多次5ALA介导光动力下存活鼻咽癌细胞的生物学行为分析

山东大学耳鼻喉眼学报 ›› 2012, Vol. 26 ›› Issue (3): 33-36.

多次5ALA介导光动力下存活鼻咽癌细胞的生物学行为分析

胡可锦1, 唐瑶云2, 赵素萍2

1.韶关学院医学院临床医学系，广东韶关 512026；2.中南大学附属湘雅医院耳鼻咽喉头颈外科，长沙 410008

收稿日期:2012-03-13 出版日期:2012-06-16 发布日期:2012-06-16
通讯作者: 唐瑶云。Email:shiw888@yahoo.com.cn
作者简介:胡可锦。Email:hkzn2002@yahoo.cn

Biological behavior evaluation of nasopharyngeal carcinoma cells that survived from multiple 5-ALA mediated photodynamic therapies

HU Ke-jin1, TANG Yao-yun2, ZHAO Su-ping2. 1

1. Department of Clinical Medicine, Medical College, Shaoguan University, Shaoguan 512026, Guangdong, China; 2. Department of Otolaryngology & Head and Neck Surgery, Affiliated Xiangya Hospital, Central South University, Changsha 410008, China

Received:2012-03-13 Online:2012-06-16 Published:2012-06-16

摘要/Abstract

摘要：

目的评价多次5-氨基乙酰丙酸（5-aminolevulinic acid, 5-ALA）介导光动力下（5-ALA-PDTs）存活鼻咽癌（NPC）细胞亚株CNE2和5-8F的生物学行为。方法采用半数致死剂量（LD50）对细胞亚株CNE2和5-8F间断进行3次PDT处理，收集存活细胞传代扩增，然后分别评价细胞增殖、黏附、迁移能力和血管生成素1、2(Ang-1, Ang-2)表达的变化。结果多次5-ALA-PDTs后存活的CNE2和58F在增殖、粘附、迁移能力上均有下降，Ang-1和Ang-2表达下调（P＜0.05）。结论多次5-ALA-PDTs可使存活NPC细胞的恶性生物学行为稳定下降，具有减少淋巴转移的潜在价值。

关键词: 光动力学治疗；鼻咽癌；生物学行为；血管生成素

Abstract:

Objective To investigate the biological behavior of nasopharyngeal carcinoma(NPC）cell CNE2 and 5-8F that survived from multiple 5-aminolevulinic acid (5-ALA) mediated PDTs (5-ALA-PDTs). Methods CNE2 and 5-8F cells were discontinuously treated with median lethal dosage PDT 3 times, surviving cells were collected for culture, then to evaluate the change of proliferation, migration, adhesion and expressions of Ang-1 and Ang-2 in these cells were evaluated. Results The CNE2 and 5-8F cells that survived from multiple 5-ALA-PDTs showed a lower level of proliferation, migration, adhesion and expressions of Ang-1 and Ang-2（P＜0.05）. Conclusion Multiple 5-ALA-PDTs could stably decrease the malignant biological behavior of surviving NPC cells, having potential value of reducing lymphatic metastasis.

Key words: Photodynamic therapy; Nasopharyngeal carcinoma; Biologic behaviors; Angiopoietin

中图分类号:

R766

胡可锦1, 唐瑶云2, 赵素萍2. 多次5ALA介导光动力下存活鼻咽癌细胞的生物学行为分析[J]. 山东大学耳鼻喉眼学报, 2012, 26(3): 33-36.

HU Ke-jin1, TANG Yao-yun2, ZHAO Su-ping2. 1. Biological behavior evaluation of nasopharyngeal carcinoma cells that survived from multiple 5-ALA mediated photodynamic therapies[J]. JOURNAL OF SHANDONG UNIVERSITY (OTOLARYNGOLOGY AND OPHTHALMOLOGY), 2012, 26(3): 33-36.

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