53例慢性化脓性中耳炎患者分泌物细菌培养及药敏学分析

doi:10.6040/j.issn.1673-3770.0.2014.396

山东大学耳鼻喉眼学报 ›› 2015, Vol. 29 ›› Issue (3): 31-34.doi: 10.6040/j.issn.1673-3770.0.2014.396

53例慢性化脓性中耳炎患者分泌物细菌培养及药敏学分析

李慎玲¹, 张孝田², 姜晓丹¹, 黄天桥¹, 郭鸽¹, 梁大鹏¹

1. 青岛大学附属医院耳鼻喉科, 山东青岛 266500;
2. 青岛大学附属医院麻醉科, 山东青岛 266500

收稿日期:2014-12-11 修回日期:2015-04-07 发布日期:2015-06-16
通讯作者: 梁大鹏。E-mail:zhangxt1984@126.com E-mail:zhangxt1984@126.com
作者简介:李慎玲。E-mail:shenlingli8512@163.com

Pathogenic bacteria and drug sensitivity in 53 patients with chronic suppurative otitis media

LI Shenling¹, ZHANG Xiaotian², JIANG Xiaodan¹, HUANG Tianqiao¹, GUO Ge¹, LIANG Dapeng¹

1. Department of Otolaryngology, The Affiliatied Hospital of Qingdao University, Qingdao 266500, Shandong, China;
2. Department of Anesthesiology, The Affiliatied Hospital of Qingdao University, Qingdao 266500, Shandong, China

Received:2014-12-11 Revised:2015-04-07 Published:2015-06-16

摘要/Abstract

摘要： 目的分析53例慢性化脓性中耳炎患者分泌物细菌分布及药敏学的数据结果, 探讨此类患者的临床用药方案。方法采用VITEK-2 COMPACT微生物分析系统对近3年住院的53例慢性化脓性中耳炎患者分泌物进行病原菌分离鉴定与药敏试验, 进行总结统计。结果 53份分泌物培养出微生物的有45份, 检出率为84.9%, 共培养出微生物49株, 其中革兰阳性球菌21株, 占42.9%;革兰阴性杆菌 21株, 占42.9%;真菌7株, 占14.2%。检出主要病原菌为铜绿假单胞菌, 占22.4%, 其次为表皮葡萄球菌、金黄色葡萄球菌, 分别占16.3%、14.3%。常见革兰阳性菌、革兰阴性菌对临床常用药物左氧氟沙星均有较高敏感性(分别为81.0%、90.9%)。结论铜绿假单胞菌、表皮葡萄球菌、金黄色葡萄球菌是常见慢性化脓性中耳炎感染细菌, 对临床常用药物(如左氧氟沙星)有较高敏感性。而对常用药物治疗不敏感的病例要警惕细菌耐药可能, 及时行细菌培养, 真菌感染不容忽视。

关键词: 药敏实验, 细菌培养, 化脓性中耳炎, 用药方案

Abstract: Objective To investigate the pathogenic bacteria and drug sensitivity tests in patients with chronic suppurative otitis media for drug application. Methods Secretion were collected from 53 patients with chronic suppurtive otitis media who were admitted into our hospital from March 2011 to March 2014.Bacterial culture and drug susceptibility tests using VITEK-2 COMPACT microbiological analysis system were performed. Results There were 49 stains of microbes isolated from 45 secretion specimens of middle ear. The first three most common pathogenic bacteria were Pseudomonas aeruginosa, Staphylococcus epidermidis and Staphylococcus aureus accounted for 22.4%, 16.3%, 16.3%, respectively. The common gram-positive and gram-negative bacteria all showed highly sensitive to levofloxacin. (81%, 90.9% correspondingly). Conclusion Pseudomonas aeruginosa, Staphylococcus epidermidis and Staphylococcus aureus are main pathogenic bacteria for chronic suppurative otitis media in our region, which are highly sensitive to general antibiotics. Culture and drug sensitivity test have great value for clinical drug application. The fungus infection can't be neglected.

Key words: Drug application, Drug sensitivity test, Suppurative otitis media, Bacterical culture

中图分类号:

R764.21

李慎玲, 张孝田, 姜晓丹, 黄天桥, 郭鸽, 梁大鹏. 53例慢性化脓性中耳炎患者分泌物细菌培养及药敏学分析[J]. 山东大学耳鼻喉眼学报, 2015, 29(3): 31-34.

LI Shenling, ZHANG Xiaotian, JIANG Xiaodan, HUANG Tianqiao, GUO Ge, LIANG Dapeng. Pathogenic bacteria and drug sensitivity in 53 patients with chronic suppurative otitis media[J]. JOURNAL OF SHANDONG UNIVERSITY (OTOLARYNGOLOGY AND OPHTHALMOLOGY), 2015, 29(3): 31-34.

参考文献

[1] 胡峰, 黄彩忠, 黄闻军, 等.117例慢性化脓性中耳炎患者的病原菌分布及药敏性分析[J].中华医院感染学杂志, 2012, 22(8):1742-1744. HU Feng, HUANG Caizhong, HUANG Wenjun. Distrbution and drug sensitivity of pathogens in 117 patients with suppurative otitis media[J]. Chin J Nosocomiol, 2012, 22(8):1742-1744.
[2] 叶满.耳部分泌物的细菌学及药敏分析[J].中华医院感染学杂志, 2010, 20(23):3822-3823. YE Man. Bacteriology of ear secretion and its drug sensitivity[J]. Chin J Nosocomiol, 2010, 20(23):3822-3823.
[3] 杨燕珍, 龚树生, 刘莹.慢性化脓性中耳炎分泌物的细菌培养及药敏实验[J].临床耳鼻咽喉科杂志, 2001, 15(12):550-552. YANG Yanzhen, GONG Shusheng, LIU Ying. The clinical investigation of bacteriology of chronic suppurative otitis media[J]. J Clin Otorhinolaryngol(China), 2001, 15(12):550-552.
[4] 张秀秀, 张秋贵, 朱富高, 等.耳部炎性疾病的细菌学及药物敏感性[J].山东大学耳鼻喉眼学报, 2009, 23(1):48-51. ZHANG Xiuxiu, ZHANG Qiugui, ZHU Fugao, et al. Pathogenic bacterium and its resistance in aural inflammatory diseases[J]. J Otolaryngol Ophthalmol Shandong Univ, 2009, 23(1):48-51.
[5] 褚莉萍, 齐少恒, 王洪飞.慢性化脓性中耳炎156例脓培养及药敏试验分析[J].蚌埠医学院学报, 2014, 39(8):1018-1023. CHU Liping, QI Shaoheng, WANG Hongfei. The pus cultivation and drug sensitive test analysis of chronic suppurative otitis media in 156 cases[J]. J Bengbu Med Coll, 2014, 39(8):1018-1023.
[6] 马均宝, 崔东岚, 吴智刚, 等.MRSA与MRCNS的淋巴感染特点及其耐药性分析[J].国际检验医学杂志, 2009, 30(8):756-759. MA Junbao, CUI Donglan, WU Zhigang, et al. Characteristic of clinical infection and drug resistance of MRSA and MRCNS[J]. Int J Lab Med, 2009, 30(8):756-759.
[7] 朱笑频, 楼正才.慢性化脓性中耳炎病原学研究及其临床应用[J].山东大学耳鼻喉眼学报, 2006, 20(5):397-400. ZHU Xiaopin, LOU Zhengcai. Pathogens of chronic suppurative otitis media and their clinical significances[J]. J Otolaryngol Ophthalmol Shandong Univ, 2006, 20(5):397-400.
[8] 胡旭武.慢性化脓性中耳炎合并真菌感染的临床分析[J].中华医院感染学杂志, 2011, 21(9):1790-1791. HU Xuwu. Clinical analysis of chronic suppurative otitis media combined with fungal infection[J]. Chin J Nosocomiol, 2011, 21(9):1790-1791.
[9] Ahn J H, Kim M N, Suk Y A, et al. Preoperative, intraoperative, and postoperative results of bacterial culture from patients with chronic suppurative otitis media[J]. Otol Nerotol, 2012, 33(1):54-59.

多维度评价

Viewed

Full text

Abstract

Cited

Shared

Discussed

53例慢性化脓性中耳炎患者分泌物细菌培养及药敏学分析

Pathogenic bacteria and drug sensitivity in 53 patients with chronic suppurative otitis media

PDF (PC)

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 9

多维度评价

本文评价

推荐阅读 0

[1]	李兆生, 洪斌, 黄前进, 蒲伟民, 许振跃. 耳源性小脑脓肿2例并文献复习[J]. 山东大学耳鼻喉眼学报, 2015, 29(5): 32-37.
[2]	陈泽, 钟兆棠, 梁敏志. 前壁外耳道-鼓膜瓣应用于开放式鼓室成形术31例[J]. 山东大学耳鼻喉眼学报, 2014, 28(6): 11-15.
[3]	房修岭, 姜虎林, 吴建霞, 韩旭巍, 张辽. 0.5%聚维酮碘冲洗结膜囊预防白内障术后眼内炎[J]. 山东大学耳鼻喉眼学报, 2014, 28(5): 66-67.
[4]	王进，何跃. 慢性化脓性中耳炎及中耳胆脂瘤术前颞骨CT及耳内镜评估的意义[J]. 山东大学耳鼻喉眼学报, 2013, 27(6): 45-47.
[5]	周洁，何源萍，曾旭东，潘芳名，李泽文 . 手术治疗儿童慢性化脓性中耳炎82例[J]. 山东大学耳鼻喉眼学报, 2009, 23(2): 45-46 .
[6]	张秀秀，张秋贵，朱富高，张晓恒 . 耳部炎性疾病的细菌学及药物敏感性分析[J]. 山东大学耳鼻喉眼学报, 2009, 23(1): 48-51 .
[7]	高春荣,闫永红,柴相君 . 喉癌患者气管切开术后痰细菌培养与药敏分析[J]. 山东大学耳鼻喉眼学报, 2007, 21(6): 498-499 .
[8]	朱笑频,楼正才 . 慢性化脓性中耳炎病原学研究及其临床应用[J]. 山东大学耳鼻喉眼学报, 2006, 20(5): 397-398 .
[9]	王国义,阎俊新,周宣岩,邹建平 . 腭裂与中耳功能障碍的相关性[J]. 山东大学耳鼻喉眼学报, 2006, 20(5): 403-405 .