姜黄素联合白藜芦醇抑制人头颈部肿瘤细胞系增殖的机制研究

doi:10.6040/j.issn.1673-3770.0.2016.425

山东大学耳鼻喉眼学报 ›› 2017, Vol. 31 ›› Issue (2): 67-72.doi: 10.6040/j.issn.1673-3770.0.2016.425

姜黄素联合白藜芦醇抑制人头颈部肿瘤细胞系增殖的机制研究

周华群¹,张立庆¹,徐朝琪²,姜盼²,王愿¹,刘晓静¹,董伟达¹

1. 南京医科大学第一附属医院/江苏省人民医院耳鼻咽喉科, 江苏南京 210029;
2. 南京医科大学公共卫生学院营养与食品卫生学系, 江苏南京 211166

收稿日期:2016-10-09 出版日期:2017-04-16 发布日期:2017-04-16
通讯作者: 董伟达. E-mail:weidadong2649@126.com
基金资助:
江苏省卫生计生委面上课题(编号H201603)

Curcumin combined with resveratrol enhances their anti-tumoral effects in the head and neck carcinoma.

1.Department of Otorhinolaryngology, First Affiliated Hospital of Nanjing Medical University/Jiangsu Provincial Peoples Hospital, Nanjing 210029, Jiangsu, China;2.Department of Nutrition and Food Hygiene, School of Public Health, Nanjing Medical University, Nanjing 211166, Jiangsu, China

Received:2016-10-09 Online:2017-04-16 Published:2017-04-16

摘要/Abstract

摘要： 目的探讨姜黄素联合白藜芦醇抑制人头颈部肿瘤细胞系增殖的机制。方法用姜黄素和白藜芦醇联合处理Hep2(人喉癌细胞株)及FaDu(人下咽癌细胞株),应用MTT(塞唑蓝)比色法检测细胞增殖抑制率,平板克隆增殖实验检测单个细胞集落形成能力,Hoechst33258染色检测细胞凋亡情况,同时应用Real-time PCR法检测Bax和Bcl-2 的mRNA 表达水平。结果姜黄素和白藜芦醇对Hep2和FaDu细胞增殖有抑制作用,二者联合处理后,对细胞增殖、细胞克隆形成能力及细胞凋亡的抑制均明显增强;同时,姜黄素和白藜芦醇上调细胞中Bax和下调Bcl-2的mRNA水平。结论姜黄素与白藜芦醇联合处理可抑制Hep2及FaDu细胞增殖,作用机制可能与上调Bax、下调Bcl-2基因表达有关。

关键词: 白藜芦醇, 增殖, 姜黄素, FaDu细胞, Hep2细胞, 凋亡

Abstract: Objective To study the effect of curcumin in combination of resveratrol on proliferation in the head and neck neoplasm cell lines. Methods Hep2 cells and FaDu cells were treated with Curcumin and resveratrol. MTT was used to determine cell proliferation. Colony formation was used to detect the cell viability. Hoechst 33258 staining was performed to observe the cell apoptosis. The mRNA expressions of Bax and Bcl-2 were analyzed by Real-time PCR. Results Hep2 and FaDu cell proliferation could be inhibited with curcumin and resveratrol in a dose-dependent manner. Their combination exhibited the best inhibitory effects. Curcumin combined with resveratrol exhibited colony repression and showed a more significant apoptosis in Hep2 and FaDu cells. Real-time PCR demonstrated that Bax mRNA was upregulated and Bcl-2 mRNA was downregulated by curcumin and resveratrol. Meanwhile, Bax mRNA was upregulated and Bcl-2 mRNA was downregulated by curcumin and resveratrol. Conclusion The combination of curcumin and resveratrol can inhibit the proliferation of Hep2 and FaDu cells more significantly. The potential mechanism may be related with the up-regulation of Bax and the down-regulation of Bcl-2.

Key words: Proliferation, Curcumin, Resveratrol, Hep2 cells, FaDu cells, Apoptosis

中图分类号:

R739.91

周华群,张立庆,徐朝琪,姜盼,王愿,刘晓静,董伟达. 姜黄素联合白藜芦醇抑制人头颈部肿瘤细胞系增殖的机制研究[J]. 山东大学耳鼻喉眼学报, 2017, 31(2): 67-72.

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姜黄素联合白藜芦醇抑制人头颈部肿瘤细胞系增殖的机制研究

Curcumin combined with resveratrol enhances their anti-tumoral effects in the head and neck carcinoma.

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