突触囊泡蛋白2A在大鼠中枢听觉系统的差异表达

doi:10.6040/j.issn.1673-3770.1.2019.004

摘要/Abstract

摘要： 目的

研究SD大鼠中枢听觉系统中各个亚区突触囊泡蛋白2A（SV₂A）的差异表达，寻找区域特异性蛋白。

方法

采用Western blot和免疫组化对SD大鼠的下丘（IC）、耳蜗核（CN）、上橄榄核复合体（SOC）、剩余脑组织包括大脑和小脑（Rest）这4个区域中SV₂A表达量进行检测，然后比较各组之间SV₂A表达量的差异。

结果

Western blotting结果显示SV₂A表达量，CN与Rest、IC、SOC各组相比差异有统计学意义(P=0.006、P=0.019、P=0.013)，而IC与Rest（P=0.802）、SOC与Rest（P=0.928）、IC与SOC（P=0.99）相比，尚不能认定有差异，根据实验数据可判定SV₂A在CN的表达量高于Rest、IC、SOC各组。免疫组化结果同样显示SV₂A表达量，CN与Rest、IC、SOC各组相比差异有统计学意义(P=0.003、P=0.016、P＜0.001)，而IC与Rest（P=0.94）、SOC与Rest（P=0.863）、IC与SOC（P=0.536）相比，尚不能认定有差异，根据实验数据可判定SV₂A在CN的表达量高于Rest、IC、SOC各组。因此表明SV₂A为CN区域特异性蛋白。

结论

SV₂A在大鼠中枢听觉核团CN中高表达，SV₂A可作为CN区域特异性蛋白，推测SV₂A可能在CN的听觉信息处理过程中发挥重要作用，这为进一步在分子水平探索CN的生理功能和CAPD病因提供了新的线索。

关键词: 突触囊泡蛋白2A, 蛋白质, 耳蜗核, 中枢听觉处理

Abstract: Objective

To study the differential expression of synaptic vesicle glycoprotein 2A (SV₂A) in various subregions of the central auditory system (CAS) of SD rats and to search for region-specific proteins.

Methods

Western blotting and immunohistochemistry were used to detect the expression of SV₂A in the inferior colliculus (IC), cochlear nucleus (CN), superior olivary nucleus complex (SOC), and the remaining brain tissues including brain and cerebellum (Rest) of SD rats. One-way analysis of variance was used to compare the differences in protein expression between the groups.

Results

The results of western blotting showed that the expression of SV₂A in CN was higher than that in Rest, IC, and SOC, and the differences were statistically significant (P = 0.006, P = 0.019, and P = 0.013, respectively). However, there were no statistically significant differences between IC and Rest (P = 0.802), SOC and Rest (P = 0.928), and IC and SOC (P = 0.99). The immunohistochemistry results also indicated that the expression of SV₂A in CN was higher than that in Rest, IC, and SOC, and the differences were statistically significant (P = 0.003, P = 0.016, and P＜0.001, respectively). However, there were no statistically significant differences between IC and Rest (P = 0.94), SOC and Rest (P = 0.863), and IC and SOC (P = 0.536). Therefore, SV₂A can be considered as a region-specific protein of CN.

Conclusion

The high expression of SV₂A in CN of rats suggests that SV₂A may be involved in the processing of auditory information in the CN, which provides a new clue for further exploring the physiological function of CN and the etiology of central auditory processing disorder (CAPD) at the molecular level.

Key words: Synaptic vesicle glycoprotein 2A, Protein, Cochlear nucleus, Central auditory processing

中图分类号:

R764.35

刘鹏利,张璐,李晓璐. 突触囊泡蛋白2A在大鼠中枢听觉系统的差异表达[J]. 山东大学耳鼻喉眼学报, 2019, 33(3): 56-60.

Pengli LIU,Lu ZHANG,Xiaolu LI. Differential expression of synaptic vesicle glycoprotein 2A in central auditory system of rats[J]. Journal of Otolaryngology and Ophthalmology of Shandong University, 2019, 33(3): 56-60.

图/表 2

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