山东大学耳鼻喉眼学报 ›› 2020, Vol. 34 ›› Issue (3): 75-80.doi: 10.6040/j.issn.1673-3770.1.2020.022

• 临床研究 • 上一篇    下一篇

BRAFV600E突变比值在合并桥本甲状腺炎甲状腺乳头状癌中的初步研究

倪烨钦1,吴凡1,荀延萍2,赵盼3,张仕蓉2,周天晗1,孙思涵4,陆凯宁4,罗定存5   

  1. 1. 浙江中医药大学第四临床医学院, 浙江 杭州 310006;
    2. 浙江大学医学院附属杭州市第一人民医院 转化医学研究中心, 浙江 杭州 310006;
    3. 浙江大学医学院附属杭州市第一人民医院 病理科, 浙江 杭州 310006;
    4. 南京医科大学, 江苏 南京 210000;
    5. 浙江大学医学院附属杭州市第一人民医院 肿瘤外科, 浙江 杭州 310006
  • 发布日期:2020-06-29
  • 通讯作者: 罗定存. E-mail:ldc65@163.com
  • 基金资助:
    杭州市重大科技创新专项项目(20131813A08);杭州市卫生科技计划项目(OO20190490、OO20190151)

Clinical effect of Hashimoto thyroiditis on the ratio of BRAFV600E alleles in papillary thyroid carcinoma

NI Yeqin1, WU Fan1, XUN Yanping2, ZHAO Pan3, ZHANG Shirong2, ZHOU Tianhan1, SUN Sihan4, LU Kaining4, LUO Dingcun5   

  1. 1. The Fourth Clinical Medical College, Zhejiang Chinese Medical University, Hangzhou 310006, Zhejiang, China;
    2. Centre of Translational Medicine, Affiliated Hangzhou First People's Hospital, Zhejiang University School of Medicine, Hangzhou 310006, Zhejiang, China;
    3. Department of Pathology, Affiliated Hangzhou First People's Hospital, Zhejiang University School of Medicine, Hangzhou 310006, Zhejiang China;
    4. Nanjing Medical University Nanjing 210000, Jiangsu, China;
    5. Department of Surgical Oncology, Affiliated Hangzhou First People's Hospital, Zhejiang University School of Medicine, Hangzhou 310006, Zhejiang, China;
  • Published:2020-06-29

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摘要： 目的 探索桥本甲状腺炎(HT)对甲状腺乳头状癌(PTC)中BRAFV600E突变比值(the ratio of BRAFV600E alleles)的影响。 方法 收集242例PTC患者临床资料,其中PTC合并HT 63例,PTC不合并HT 179例,采用微滴数字PCR(ddPCR)法检测PTC中BRAFV600E状况。利用倾向性评分匹配法(PSM),以性别、年龄、多灶性、腺外侵犯、肿瘤直径为控制变量按1∶1比例构成63对配对,比较匹配后两组BRAFV600E阳性率和突变比值。 结果 倾向性评分匹配前,PTC合并HT组和PTC不合并HT组BRAFV600E阳性率分别为87.3%和92.7%,两组间差异无统计学意义(P>0.05),而PTC合并HT组BRAFV600E突变比值(3.20%)显著低于PTC不合并HT组(8.80%)(P<0.001)。经过倾向性评分匹配,两组基线水平保持一致,PTC合并HT组BRAFV600E突变比值(3.20%)仍显著低于PTC不合并HT组(9.00%),两组间差异有统计学意义(P=0.001)。 结论 在合并HT的PTC中,BRAFV600E突变的发生未受影响,但是BRAFV600E突变比值明显降低,说明HT对PTC发展过程中BRAFV600E突变存在一定影响,从而影响PTC发展及其预后。

关键词: 甲状腺肿瘤, 倾向性评分匹配, 桥本甲状腺炎, BRAFV600E突变比值

Abstract: Objective To evaluate the clinical effect of Hashimoto thyroiditis(HT)on the ratio of BRAFV600E alleles in papillary thyroid carcinoma(PTC). Methods Two hundred and forty-two patients who underwent surgery at our hospital between December 2017 and December 2019 were enrolled in this study. The ratio of BRAFV600E alleles was measured using droplet digital PCR and formalin-fixed paraffin-embedded tissues, and its correlation with HT was analyzed. Using propensity score matching and based on the 63 patients with HT(1∶1), 179 PTC patients without HT during the same period were retrospectively enrolled as controls. Results There was a significant difference between the ratio of BRAFV600E alleles(P<0.001)of the two groups(PTC patients with HT versus those without HT); the rates of positive-BRAFV600E were not significantly different(87.3% vs 92.7%, P>0.05). After propensity score matching, the significant difference between the two groups at baseline was no longer observed. The ratio of BRAFV600E alleles was still significantly lower in patients with HT(median 3.20%)than in those without HT(median 9.00%, P=0.001). Conclusion HT can significantly reduce the ratio of BRAFV600E alleles in PTC, and this has an important effect on PTC progression and prognosis.

Key words: Thyroid neoplasms, Propensity score matching, Hashimoto thyroiditis, Ratio of BRAFV600E alleles

中图分类号: 

  • R604
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