山东大学耳鼻喉眼学报 ›› 2014, Vol. 28 ›› Issue (5): 1-3.doi: 10.6040/j.issn.1673-3770.0.2014.139

• 睡眠呼吸障碍性疾病 •    下一篇

褪黑素对慢性间歇性缺氧性心肌cTnT及 Ca2+-ATPase的影响

董译元, 凌月福, 张耀庭   

  1. 桂林医学院附属医院耳鼻咽喉头颈外科, 广西 桂林 541001
  • 收稿日期:2014-04-28 出版日期:2014-10-16 发布日期:2014-10-16
  • 通讯作者: 凌月福，教授。E-mail:Lingyuefu@yahoo.com.cn E-mail:Lingyuefu@yahoo.com.cn
  • 作者简介:董译元。E-mail:tiandd1977@tom.com
  • 基金资助:
    广西壮族自治区卫生厅科研课题(Z2010285)

Effect of melatonin on the cTnT and Ca2+-ATPase of chronic intermittent hypoxia cardiac muscular tissues

DONG Yi-yuan, LING Yue-fu, ZHANG Yao-ting   

  1. Department of Otorhinolaryngology & Head and Neck Surgery, Affiliated Hospital of Guilin Medical College, Guilin 541001, Guangxi, China
  • Received:2014-04-28 Online:2014-10-16 Published:2014-10-16

PDF (PC)

344

摘要： 目的 探讨褪黑素(MLT)对常压下慢性间歇性缺氧SD大鼠心肌的保护作用。方法 将24只雄性SD大鼠(5周龄)随机分成3组:对照组、实验A组、实验B组。对照组在空气中正常饲养，实验A组和实验B组在常压慢性间歇性低氧箱内，对照组和实验B组灌服生理盐水，实验A组灌服褪黑素。8周后用光电比色法测定血清肌钙蛋白T(cTnT)的浓度和心肌钙离子ATP酶(Ca2+-ATPase)的活性，取大鼠右心室做病理切片。结果 ①实验B组与实验A组比较：实验B组的cTnT明显增加(P<0.05)，心肌组织的Ca2+-ATPase的活性明显降低(P<0.05)。病理切片显示,个别心肌细胞核消失，心肌细胞肥大，心肌之间有大量出血；②实验A组与对照组比较：cTnT和Ca2+-ATPase均无明显差异(P>0.05)。病理切片显示，心肌细胞之间有少量出血；③实验B组与对照组比较：实验B组的cTnT明显增加(P<0.05)，心肌组织的Ca2+-ATPase的活性明显降低(P<0.05)。病理切片显示,个别心肌细胞核消失，心肌细胞肥大，心肌之间有大量出血。结论 褪黑素对慢性间歇性缺氧心肌具有保护作用。

关键词: 肌钙蛋白T, 慢性间歇性缺氧, 心肌钙离子ATP酶, 褪黑素

Abstract: Objective To investigate whether the melatonin (melatonin, MLT) could protect SD rats' cardiac muscular tissues from chronic intermittent hypoxia damage in the atmospheric pressure. Methods The 24 male, SD rats(5 weeks) were randomly divided into three groups: the Control group, the experimental group A and the experimental group B. The control group rats were fed with normally reared in the air, and the experimental group A and B rats were living in the atmospheric pressure and chronic intermittent hypoxia boxes. The control group rats and the experimental group B rats were fed with normal saline solution(NS), and the experimental group A rats were fed with melatonin (MLT). After 8 weeks, the cTnT capacity and Ca2+- ATPase activity of each rat were detected, and the right ventricles were taken for pathological section. Results ①Comparison of the experimental group B and the experimental group A: The cTnT capacity of the experimental group B was increased (P<0.05), and the Ca2+- ATPase activity of the experimental group B was decreased (P<0.05). Individual myocardial cell nuclei disappeared. Myocardial cells were hypertrophy and there was a large amount of bleeding between myocardial. ②Comparison of the experimental group A and the control group: The above indicators had no obvious differences (P>0.05). There was a small amount of bleeding between myocardial. ③Comparison of the experimental group B and the control group: The cTnT capacity of the experimental group B was increased (P<0.05), and the Ca2+-ATPase activity of the experimental group B was decreased (P<0.05). Individual myocardial cell nuclei disappeared. Myocardial cells were hypertrophy, and there was a large amount of bleeding in myocardial. Conclusion MLT can protect rats' cardiac muscular tissue from injury of chronic intermittent hypoxia.

Key words: Chronic intermittent hypoxia, cTnT, Ca2+-ATPase, Melatonin

中图分类号: 

  • R762
[1] 周敬,鲁沈源,励雯静,等. 148例鼾症及阻塞性睡眠呼吸暂停低通气综合征危险因素的分析[J]. 复旦学报：医学版,2010,55(2):207-210.ZHOU Jing, LU Shenyuan, LI Wenjing, et al. Analysis of the clinicl characteristics in 148 patients with snoring and obstructive sleep apnea hypopnea syndrome[J]. Fudan University J Med Sci, 2010, 55(2):207-210.
[2] 王鹏,李海涛,白华. 褪黑素与心血管疾病相关性研究进展[J]. 中国循证心血管医学杂志, 2009,1(2):115-118.WANG Peng, LI Haitao, BAI Hua. Correlation between melatonin and cardiovascular diseases[J]. Chin J Evidence-Based Cardiovascular Med, 2009, 1(2):115-118.
[3] 张耀庭,凌月福.褪黑素对慢性间歇性缺氧心肌保护作用[J].临床医学工程,2012,19(9):1626-1628.ZHANG Yaoting, LING Yuefu. Protective effect of melatonin on chronic intermittent hypoxia cardiac muscular tissue[J]. Clin Med Engineering, 2012, 19(9):1626-1628.
[4] 王旭禛,凌月福. 褪黑素对常压下慢性缺氧性心肺的保护作用[J].中国医药导刊,2011,13(11)：1933-1935.WANG Xuzhen, LING Yuefu. Protective effect of melatonin on atmospheric pressure of chronic hypoxic heart and lung[J]. Chin Med Guide, 2011,13 (11):1933-1935.
[5] Song F, Jia W, Yao Y, et al.Oxidative stress,antioxidantstatus and DNA damage in patients with impaired glucoseregulation and newly diagnosed type 2 diabetes [J].Clin Sci(Lond), 2007, 112(12):599-606.
[6] Kaneto H, Katakami N, Kawamori D, et al. Involvement of oxidative stress in the pathogenesis of diabetes[J].Antioxid Redox Signal, 2007, 9(3):355-366.
[7] 曾爱辉, 谭茗月. 肌钙蛋白T与慢性心力衰竭的关系[J]. 心血管病学进展,2011,32(3):371-373. ZENG Aihui, TAN Mingyue. The relationship between cardiac troponint and chronic heart failure[J]. Advances Cardiovascular DiS, 2011,32(3):371-373.
[1] 宋西成. 加速康复外科理念在头颈肿瘤手术的应用价值[J]. 山东大学耳鼻喉眼学报, 2018, 32(5): 1-4.
[2] 孙莎莎,邱杰,臧传善,孙彦. 白塞病误诊为急性会厌炎两例[J]. 山东大学耳鼻喉眼学报, 2017, 31(5): 107-109.
[3] 郭颖媛,张德军,管国芳,文连姬,张辉,于多娇,马帅帅. 3D打印技术在耳鼻喉科住院医师规范化培训中的应用探索[J]. 山东大学耳鼻喉眼学报, 2017, 31(3): 119-122.
[4] 刘文静. TransPRK与LASEK对薄角膜近视眼术后视力、屈光度及角膜生物力学性能的影响[J]. 山东大学耳鼻喉眼学报, 2016, 30(6): 70-74.
[5] 王亚琳综述,陈剑秋审校. 声带白斑与癌前病变关系探讨及其研究进展[J]. 山东大学耳鼻喉眼学报, 2016, 30(6): 90-93.
[6] 谢畅,颜滨,李建兴,卢永田. 内镜经口入路寰枢椎解剖及临床可行性研究[J]. 山东大学耳鼻喉眼学报, 2016, 30(3): 47-51.
[7] 孟庆国, 卢永田, 伍小六, 李建兴, 孙焕吉, 张宝莹, 谢畅. 腭鞘管后部的浅沟:定位腭鞘管的解剖标记[J]. 山东大学耳鼻喉眼学报, 2015, 29(5): 55-61.
[8] 李丹凤, 陈福权, 石力, 陈俊. 阶梯式多模式融合教学法在耳鼻喉科本科临床教学中的应用[J]. 山东大学耳鼻喉眼学报, 2015, 29(4): 77-79.
[9] 严羽, 朱江. 早期喉癌及喉癌前病变诊断的研究进展[J]. 山东大学耳鼻喉眼学报, 2015, 29(4): 80-85.
[10] 苟栋明1，王海1，陈建超2. 近发际缘内头皮冠状切口径路在耳鼻咽喉头颈外科手术中应用的临床意义[J]. 山东大学耳鼻喉眼学报, 2014, 28(1): 62-64.
[11] 吴喜福，黄健聪，庄士民，黄子真，叶进，张革化. 颈部坏死性筋膜炎1例并文献复习[J]. 山东大学耳鼻喉眼学报, 2014, 28(1): 89-90.
[12] 黄郁林，梁健刚. 木村病1例[J]. 山东大学耳鼻喉眼学报, 2012, 26(4): 89-90.
[13] 秦贺,孙建军. 脱细胞真皮基质在耳鼻咽喉修复外科中的应用[J]. 山东大学耳鼻喉眼学报, 2012, 26(4): 75-77.
[14] 郝凯飞，陶树东，韩冰，闫朝晖. 头颈部皮肤恶性肿瘤16 例[J]. 山东大学耳鼻喉眼学报, 2011, 25(3): 12-14.
[15] 欧阳天斌综述,唐世雄审校. 咽喉反流疾病发病机制及诊断的相关进展[J]. 山东大学耳鼻喉眼学报, 2010, 24(5): 48-51.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
No Suggested Reading articles found!
网站版权所有 © 2018 《山东大学耳鼻喉眼学报》编辑部　
地址：山东省济南市山大南路27号（250100）电话：+86-0531-88366912 E-mail: ebhxb@sdu.edu.cn
本系统由北京玛格泰克科技发展有限公司设计开发