TNF-α和IL-8在突发性聋患者血清及外周血单个核细胞中的表达及其意义

doi:10.6040/j.issn.1673-3770.0.2015.473

山东大学耳鼻喉眼学报 ›› 2016, Vol. 30 ›› Issue (2): 40-45.doi: 10.6040/j.issn.1673-3770.0.2015.473

TNF-α和IL-8在突发性聋患者血清及外周血单个核细胞中的表达及其意义

衡伟伟^1,2,后婕¹,魏先梅¹,佘万东¹

1.南京大学医学院附属鼓楼医院耳鼻咽喉头颈外科, 江苏南京 210008;
2.南京大学医学院附属口腔医院-南京市口腔医院耳鼻咽喉头颈外科, 江苏南京 210008

收稿日期:2015-11-09 出版日期:2016-04-16 发布日期:2016-04-16
通讯作者: 佘万东. E-mail:shewangdong@163.com E-mail:hengww006@163.co
作者简介:衡伟伟. E-mail:hengww006@163.co
基金资助:
国家自然科学基金项目(81271074);江苏省第十批“六大人才高峰项目”、江苏省临床医学科技专项(BL2014002);南京市国际联合研究项目(2012sd311038)联合资助

Expressions and significance of TNF-α and IL-8 in serum and peripheral blood mononuclear cells from patients with sudden sensorineural hearing loss.

HENG Weiwei^1,2, HOU Jie¹, WEI Xianmei¹, SHE Wandong¹

1. Department of Otolaryngology &Head and Neck Surgery, Affiliated Drum Tower Hospital, Medical College of Nanjing University, Nanjing 210008, Jiangsu, China;2. Department of Otolaryngology &Head and Neck Surgery, Nanjing Stomatological Hospital, Medical College of Nanjing University, Nanjing 210008, Jiangsu, China

Received:2015-11-09 Online:2016-04-16 Published:2016-04-16

摘要/Abstract

摘要： 目的探讨TNF-α和IL-8在突发性聋患者血清及外周血单个核细胞(PBMC)中的表达及其临床意义。方法选择突发性耳聋患者44例作为实验组,健康志愿者32例作为对照组,分别测定血清和PBMC上清液中的TNF-α和IL-8的浓度以及PBMC细胞中TNF-α和IL-8的mRNA表达水平。结果 TNF-α和IL-8在实验组患者血清中的浓度与对照组比较,差异无统计学意义(P>0.05);TNF-α和IL-8在PBMC上清液中的浓度明显增加,且在PBMC细胞中mRNA表达水平显著提高,差异具有统计学意义(P<0.05)。结论 TNF-α和IL-8的浓度在突发性聋患者体内升高与内耳的免疫介导机制密切相关,提示炎症反应在突发性聋的发生中起着重要作用,为突发性耳聋的诊断及治疗提供新思路和方法。

关键词: 免疫介导, IL-8, 外周血单个核细胞, 突发性聋, TNF-α, 炎症, 血清

Abstract: Objective To explore the expressions of tumor necrosis factor-α(TNF-α)and intedeukik-8(IL-8)in serum and peripheral blood mononuclear cells(PBMCs)from patients with sudden sensorineural hearing loss(SSNHL)and their clinical significance. Methods A total of 44 patients with SSNHL and 32 age-matched controls were enrolled in the study. The expressions of TNF-α and IL-8 in the serum and PBMCs were detected respectively. Results The expressions of TNF-α and IL-8 in the serum from patients with SSNHL were not much different from those in normal controls(P>0.05). However, they were significantly higher in PBMCs(P<0.05). Conclusion The elevated expressions of TNF-α and IL-8 in patients with SSNHL are closely related to the inner ear immune-mediation, indicating that inflammation may play an important role in the pathogenesis of the disease. The expressions of TNF-α and IL-8 may serve as novel diagnostic and therapeutic targets of SSNHL.

Key words: IL-8, Sudden sensorineural hearing loss, Inflammation, TNF-α, Immune-mediated, Serum, Peripheral blood mononuclear cell

中图分类号:

R764.4

衡伟伟,后婕,魏先梅,佘万东. TNF-α和IL-8在突发性聋患者血清及外周血单个核细胞中的表达及其意义[J]. 山东大学耳鼻喉眼学报, 2016, 30(2): 40-45.

HENG Weiwei, HOU Jie, WEI Xianmei, SHE Wandong. Expressions and significance of TNF-α and IL-8 in serum and peripheral blood mononuclear cells from patients with sudden sensorineural hearing loss.[J]. JOURNAL OF SHANDONG UNIVERSITY (OTOLARYNGOLOGY AND OPHTHALMOLOGY), 2016, 30(2): 40-45.

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TNF-α和IL-8在突发性聋患者血清及外周血单个核细胞中的表达及其意义

Expressions and significance of TNF-α and IL-8 in serum and peripheral blood mononuclear cells from patients with sudden sensorineural hearing loss.

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