山东大学耳鼻喉眼学报 ›› 2017, Vol. 31 ›› Issue (4): 43-46.doi: 10.6040/j.issn.1673-3770.0.2017.189

• 论著 • 上一篇    下一篇

嗜酸性粒细胞及IL5在上颌窦后鼻孔息肉与鼻息肉中表达的差异

郅莉莉1,宋道亮2   

  1. 1. 淄博市中心医院北院区耳鼻喉科变态反应门诊;
    2. 淄博市中心医院耳鼻喉科, 山东 淄博 255031
  • 收稿日期:2017-05-02 出版日期:2017-08-16 发布日期:2017-08-16
  • 通讯作者: 宋道亮. E-mail:songdaoliang1971@163.com

Expressions of eosinophils and IL5 in antrochoanal polyps and nasal polyps.

ZHI Lili1, SONG Daoliang2   

  1. 1. Allergic Clinic of Department of Otolaryngology, North Branch of Zibo Central Hospital;2. Department of Otolaryngology, Zibo Central Hospital, Zibo 255031, Shandong, China
  • Received:2017-05-02 Online:2017-08-16 Published:2017-08-16

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摘要： 目的 通过对比上颌窦后鼻孔息肉(ACP)、伴变应性鼻炎的鼻息肉(NPwAR)及不伴变应性鼻炎的鼻息肉(NPsAR)组织中嗜酸性粒细胞浸润及IL5的表达水平,探讨嗜酸性粒细胞及IL5在不同鼻腔炎性疾病发生中作用的差异。 方法 对10例ACP、9例NPwAR及14例NPsAR组织切片行H&E染色,对嗜酸性粒细胞的浸润进行计数;提取组织总RNA,通过实时荧光定量核酸扩增检测系统(QPCR)对组织中IL5的mRNA表达水平进行检测。 结果 嗜酸性粒细胞在ACP中的浸润显著低于NPwAR(P<0.001),嗜酸性粒细胞在ACP中浸润百分比的中位数低于NPsAR,但两者间差异无统计学意义;嗜酸性粒细胞在NPwAR中的表达水平显著高于NPsAR(P<0.05)。在ACP组织中IL5的mRNA表达水平显著低于NPsAR(P<0.05)和NPwAR(P=0.001),且NPsAR组织中IL5的mRNA表达水平显著低于NPwAR(P<0.05)。 结论 ACP与NP的发病机制有所不同。嗜酸性粒细胞及IL5参与了NP的发病过程,并与变应性鼻炎的发生密切相关,但在ACP的形成中可能未起到重要作用。

关键词: 上颌窦后鼻孔息肉, 嗜酸性粒细胞, 变应性鼻炎, 鼻息肉, 白细胞介素5

Abstract: Objective To compare eosinophil infiltration as well as the IL5 mRNA levels in antrochoanal polyps(ACP), nasal polyps with allergic rhinitis(NPwAR), and nasal polyps without allergic rhinitis(NPsAR). Methods A total of 10 patients with ACP, 9 with NPwAR, and 13 with NPsAR were enrolled in this study. Eosinophil infiltration was analyzed by hematoxylin and eosin staining. The level of IL5 mRNA was determined by quantitative PCR. Results Eosinophil infiltration in ACP was significantly less than that in NPwAR(P<0.001). Although the median eosinophil infiltration in ACP was less than that in NPsAR, the difference was not statistically significant. Eosinophil infiltration in NPsAR was significantly less than that in NPwAR(P< 0.05). The IL5 mRNA level in ACP was significantly lower than those in NPsAR(P<0.05)and NPwAR(P=0.001). In addition, the IL5 mRNA level in NPsAR was significantly lower than that in NPwAR(P<0.05). Conclusion Antrochoanal polyps may differ in their pathogenesis from NP. Eosinophils and IL5 both play a role in the etiology of NP with or without AR, but may not play important roles in the pathogenesis of ACP.

Key words: Nasal polyps, Allergic rhinitis, Eosinophils, Interleukin-5, Antrochoanal polyps

中图分类号: 

  • R765.2
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