MGMT和EGFR蛋白在喉鳞状细胞癌中的表达及临床意义

doi:10.6040/j.issn.1673-3770.0.2017.116

山东大学耳鼻喉眼学报 ›› 2017, Vol. 31 ›› Issue (4): 68-72.doi: 10.6040/j.issn.1673-3770.0.2017.116

MGMT和EGFR蛋白在喉鳞状细胞癌中的表达及临床意义

朱晓城¹,钱晓云²,顾亚军²,沈晓辉²,宋盼盼²,李惠²,高下²

1. 东南大学医学院, 江苏南京 210009;
2. 南京大学医学院附属鼓楼医院耳鼻咽喉头颈外科, 江苏南京 210008

收稿日期:2017-03-17 出版日期:2017-08-16 发布日期:2017-08-16
通讯作者: 高下. E-mail:xiagaogao@hotmail.com
基金资助:
南京市医学发展项目(YKK14062)

Expression and clinical value of MGMT and EGFR in laryngeal squamous cell carcinoma.

ZHU Xiaocheng¹, QIAN Xiaoyun², GU Yajun², SHEN Xiaohui², SONG Panpan², LI Hui², GAO Xia

1.School of Medicine, Southeast University, Nanjing 210009, Jiangsu, China;2.Department of Otolaryngology &Head and Neck Surgery, Affiliated Drum Tower Hospital of Nanjing University Medical School, Nanjing 210008, Jiangsu, China

Received:2017-03-17 Online:2017-08-16 Published:2017-08-16

摘要/Abstract

摘要： 目的探讨喉鳞状细胞癌组织中O6-甲基鸟嘌呤DNA甲基转移酶(MGMT)、表皮生长因子受体(EGFR)的表达与临床病理特征及其相互之间的关系,研究其在肿瘤发生发展以及治疗中的作用。方法采用免疫组织化学染色法检测MGMT、EGFR的表达,分析103例喉癌组织标本与临床病理特征的关系。结果 MGMT的表达与分化程度、T分期以及临床分期呈正相关(χ2为13.302、8.394、11.403,P值分别为0.001、0.039、0.010<0.05);EGFR的表达与T分期以及临床分期呈正相关(χ2为14.933、16.603,P值分别为0.002、 0.001<0.05)。分层分析发现,MGMT、EGFR 两者的表达强度存在正相关(χ2=5.369, P=0.019<0.05)。结论喉鳞状细胞癌中 MGMT、EGFR的表达与T分期、临床分期均有相关性,测定两者的表达水平可评估喉鳞状细胞癌的恶性程度并指导喉癌的治疗。

关键词: 喉鳞状细胞癌, O6-甲基鸟嘌呤DNA甲基转移酶, 表皮生长因子受体

Abstract: Objective To investigate the relationship between expression of O6-methylguanine-DNA methyltransferase(MGMT)and epidermal growth factor receptor(EGFR)in laryngeal squamous cell carcinoma(LSCC), and their relationship with clinicopathological features. Methods We collected 103 tumor tissues, detected the expression of MGMT and EGFR by immunohistochemistry(IHC), and explored their relationship with the clinicopathological features. Results The expression of MGMT in tumor tissues was positively related to differentiation degree, T stage, and clinical stage(χ2 13.302, 8.394, 11.403, P=0.001, 0.039, and 0.010, respectively). The expression of EGFR in tumor tissue was positively correlated with T stage and clinical stage(χ2 = 14.933, 16.603, P=0.002, and 0.001, respectively). Further analysis found that the expression levels of MGMT and EGFR were positively correlated(MGMT vs EGFR χ2=5.369, P=0.019). Conclusion The expression of MGMT and EGFR was correlated with T stage and clinical stage. Detecting their expression can assist in assessing the malignancy of LSCC and guide treatment.

Key words: Epidermal growth factor receptor, Laryngeal squamous cell carcinoma, O6-Methylguanine-DNA methyltransferase

中图分类号:

R739.65

朱晓城,钱晓云,顾亚军,沈晓辉,宋盼盼,李惠,高下. MGMT和EGFR蛋白在喉鳞状细胞癌中的表达及临床意义[J]. 山东大学耳鼻喉眼学报, 2017, 31(4): 68-72.

ZHU Xiaocheng, QIAN Xiaoyun, GU Yajun, SHEN Xiaohui, SONG Panpan, LI Hui, GAO Xia. Expression and clinical value of MGMT and EGFR in laryngeal squamous cell carcinoma.[J]. JOURNAL OF SHANDONG UNIVERSITY (OTOLARYNGOLOGY AND OPHTHALMOLOGY), 2017, 31(4): 68-72.

参考文献

[1] Corry J, Rischin D, Cotton S, et al. Larynx preservation with primarynon-surgical treatment for loco-regionally advanced larynx cancer[J]. J Med Imaging Radiat Oncol, 2011, 55(2): 229-235.
[2] Hashibe M, Brennan P, Benhamou S, et al. Alcohol drinking in never users of tobacco, cigarette smoking in never drinkers, and the risk of head and neck cancer: pooled analysis in the International Head and Neck Cancer Epidemiology Consortium[J]. J National Cancer Institute, 2007, 99(10):777-789.
[3] Burger PC, Green SB. Patient age, histologic features, and length of survival in patients with glioblastoma multiforme[J]. Cancer, 1987, 59(9):1617-1625.
[4] Grandis JR, Melhem MF, Barnes EL, et al. Quantitative immunohistochemical analysis of transforming growth factor-α and epidermal growth factor receptor in patients with squamous cell carcinoma of the head and neck[J]. Cancer, 1996, 78(6):1284-1292.
[5] Grandis JR, Melhem MF, Gooding WE, et al. Levels of TGF-α and EGFR protein in head and neck squamous cell carcinoma and patient survival[J]. J Natl Cancer Inst, 1998, 90(11): 824-832.
[6] Singh T, Gupta NA, Xu S, et al. Honokiol inhibits the growth of head and neck squamous cell carcinoma by targeting epidermal growth factor receptor [J]. Oncotarget, 2015, 6(25):21268-21282.
[7] Rahman WF, Rahman KS, Nafi SN, et al. Overexpression of DNA methyltransferase 1(DNMT1)protein in astrocytic tumour and its correlation with O6-methylguanine-DNA methyltransferase(MGMT)expression[J]. Int J Clin Exp Pathol, 2015, 8(6):6095-6106.
[8] Starska K, Brys M, Forma E. Impact of EGFR immunoexpression on STAT3 activation and association with proinflammatory/regulatory cytokine pattern in laryngeal squamous cell carcinoma[J]. Oncol Rep, 2009, 21(2):539-548.
[9] Christmann M, Verbeek B, Roos W, et al. O(6)-Methylguanine- DNA methyltransferase(MGMT)in normal tissues and tumors: enzyme activity, promoter methylation and immunohistochemistry[J]. Biochim Biophys Acta, 2011,1816(2):179-190.
[10] 徐红超,陶静莉,许成杰,等.MGMT VEGF在脑胶质瘤中的表达及临床意义分析[J].中国实用神经疾病杂志,2016(2):41-43.
[11] Wick W, Platten M. Understanding and targeting alkylator resistance in glioblastoma[J]. Cancer Discov, 2014, 4(10):1120-1122.
[12] Wickström M, Dyberg C,Milosevic J, et al. Wnt/β-catenin pathway regulates MGMT gene expression in cancer and inhibition of Wnt signalling prevents chemoresistance[J]. Nat Commun, 2015, 6:8904.doi: 10.1038/ncomms9904.
[13] Kontic M, Milovanovic J, Colovic Z, et al. Epidermal growth factor receptor(EGFR)expression in patients with laryngeal squamous cell carcinoma[J]. Eur Arch Otorhinolaryngol, 2015, 272(2):401-405.
[14] Agra IM, Ferlito A, Takes RP, et al. Diagnosis and treatment of recurrent laryngeal cancer following initial nonsurgical therapy[J]. Head Neck, 2012, 34(5):727-735.
[15] Orlova RV, Vaizyan RI, Ivanova AK, et al. Chemotherapy for malignant tumors: problems and prospects[J].Vopr Onkol, 2015, 61(2):244-251.
[16] Belanich M, Pastor M, Randall T, et al. Retrospective study of the correlation between the DNA repair protein alkyltransferase and survival of brain tumor patients treated with carmustine[J]. Cancer Res, 1996, 56(4):783-788.
[17] Esteller M, Garcia-Foncillas J, Andion E, et al. Inactivation of the DNA-repair gene MGMT and the clinical response of gliomas to alkylating agents[J]. N Engl J Med, 2000, 343(19):1350-1354.
[18] Tini P, Pastina P, Nardone V, et al. The combined EGFR protein expression analysis refines the prognostic value of the MGMT promoter methylation status in glioblastoma. Clin Neurol Neurosurg, 2016, 149(10):15-21.

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MGMT和EGFR蛋白在喉鳞状细胞癌中的表达及临床意义

Expression and clinical value of MGMT and EGFR in laryngeal squamous cell carcinoma.

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