山东大学耳鼻喉眼学报 ›› 2017, Vol. 31 ›› Issue (4): 76-79.doi: 10.6040/j.issn.1673-3770.0.2016.477
王慧1,温晏1,闫丽1,于晓明1,宁宏2
WANG Hui1, WEN Yan1, YAN Li1, YU Xiaoming1, NING Hong
摘要: 目的 研究TGF-β1对人晶状体上皮细胞(HLEC)上皮间质转分化(EMT)的影响。 方法 根据处理不同,将细胞分为TGF-β1组、siRNA-TGF-β1组和空白对照组,培养24 h后观察细胞形态,Transwell法检测各组细胞迁移能力,实时荧光定量PCR检测各组细胞中TGF-β1、α-SMA、E-cadherin和vimentin基因表达,Western blotting法检测各组细胞中PI3K、Akt和p-Akt蛋白表达。 结果 TGF-β1组迁移细胞数为(31.2±4.7)个,高于siRNA-TGF-β1组(14.6±5.3)个及空白对照组(3.9±1.4)个,且空白对照组高于siRNA-TGF-β1组;siRNA-TGF-β1组TGF-β1、α-SMA 及Vimentin的mRNA相对表达量均低于TGF-β1组及空白对照组,且空白对照组低于TGF-β1组;siRNA-TGF-β1组E-cadherin的mRNA相对表达量均高于TGF-β1组及空白对照组,且空白对照组高于TGF-β1组;siRNA-TGF-β1组细胞中PI3K蛋白及p-Akt蛋白相对表达量均低于TGF-β1组和空白对照组,且空白对照组低于TGF-β1组,siRNA-TGF-β1组Akt蛋白相对表达量高于TGF-β1处理组和空白对照组,且空白对照组高于TGF-β1组。 结论 TGF-β1可诱导HLEC发生EMT,且可促进HLEC迁移,其机制可能与细胞中PI3K/Akt信号通路活化有关。
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