山东大学耳鼻喉眼学报 ›› 2017, Vol. 31 ›› Issue (5): 79-84.doi: 10.6040/j.issn.1673-3770.0.2017.321

• 论著 • 上一篇    下一篇

MiR-150调控Nanog对鼻咽癌侧群细胞增殖、侵袭的影响

周毅波,龚小蓉,于锋   

  1. 广州市第十二人民医院 广州市耳鼻咽喉头颈外科医院 广州医科大学耳鼻咽喉头颈外科研究所, 广东 广州 510620
  • 收稿日期:2017-07-26 出版日期:2017-10-16 发布日期:2017-10-16
  • 通讯作者: 于锋. E-mail:fishwoo@sina.com
  • 基金资助:
    广东省医学科学技术研究基金项目(A2015235);广州市医药卫生科技项目(20141A011045)

Effects of miR-150 targeted to Nanog on proliferation and invasion of nasopharyngeal carcinoma side population cells.

ZHOU Yibo, GONG Xiaorong, YU Feng   

  1. Guangzhou 12th Peoples Hospital, Guangzhou Hospital of Otolaryngology Head and Neck Surgery, Institute of Otolaryngology Head and Neck Surgery of Guangzhou Medical University, Guangzhou 510620, Guangdong, China
  • Received:2017-07-26 Online:2017-10-16 Published:2017-10-16

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摘要： 目的 检测MiR-150在鼻咽癌侧群(SP)细胞中的表达,并探讨其是否通过调控靶基因Nanog促进鼻咽癌侧群细胞的增殖与侵袭。 方法 采用SYBR Green 实时定量荧光聚合酶链式反应(qRT-PCR)法检测MiR-150及Nanog在鼻咽癌侧群细胞和非侧群(NSP)细胞中的表达情况;并通过瞬时转染miR-150 inhibitor 至SP细胞、miR-150 mimic至NSP细胞中,western blotting检测Nanog的表达情况;进一步通过CCK-8实验、Transwall实验检测鼻咽癌侧群细胞增殖、侵袭能力的变化,数据处理采用两独立样本t检验。 结果 qRT-PCR检测结果表明,MiR-150在SP细胞(0.99±0.05)较NSP细胞(0.59±0.02)中表达水平升高(t=8.06, P<0.000 1),Nanog在鼻咽癌SP细胞(0.99±0.47)较NSP 细胞(0.49±0.05)表达升高(t=7.5, P<0.000 1);SP细胞转染MiR-150 inhibitor(0.46±0.03)较对照组(1.01±0.07)Nanog表达下调(t=6.85, P=0.000 5)、CCK-8实验示增殖受抑制、Transwell侵袭实验示侵袭能力减弱(114.40±5.14 vs 57.30±4.29, t=8.5, P<0.000 1);NSP细胞转染MiR-150 mimic(1.01±0.06)组较对照组(0.48±0.04)Nanog表达上调(t=6.16, P=0.000 8),增殖及侵袭能力增强。 结论 鼻咽癌SP细胞中MiR-150与Nanog呈高表达,MiR-150可通过调控靶基因Nanog促进鼻咽癌侧群细胞的增殖与侵袭。

关键词: SP细胞, 增殖, 侵袭, 鼻咽癌, MiR-150, NSP细胞

Abstract: Objective To detect the level of miR-150 in side population(SP)and non-side population(NSP)cells of nasopharyngeal carcinoma and to investigate whether miR-150 promoted proliferation and invasion via the Nanog gene. Method miR-150 and Nanog were detected by qRT-PCR in two cell types. An miR-150 inhibitor and miR-150 mimic were used to reduce and upregulate the level of miR-150, respectively, and CCK-8 and Transwell assays were performed to detect cell proliferation and invasion; the change in Nanog was measured by qRT-PCR and western blot analysis. A t-test for independent samples was used to analyze the data. Results The miR-150 level in SP cells(0.99±0.05)was significantly higher than that in NSP cells(0.59±0.02, t=8.06, P<0.000 1)and the level of Nanog in SP cells(0.99±0.47)was significantly higher than that in NSP cells(0.49±0.05, t=7.5, P<0.000 1). The CCK-8 and Transwell assays showed that MiR-150 inhibited proliferation and invasion(114.40±5.14 vs 57.30±4.29, t=8.5, P<0.000 1); western blotting showed that the reduction in MiR-150 decreased the protein expression of Nanog in SP cells(1.01±0.07 vs 0.46±0.03, t=6.85, P=0.000 5). In contrast, the miR-150 mimic group showed significant upregulation of Nanog in NSP cells(0.48±0.04 vs 1.01±0.06, t=6.16, P=0.000 8). Conclusion miR-150 and Nanog were highly expressed in SP cells of nasopharyngeal carcinoma; additionally, miR-150 promoted proliferation and invasion through upregulation of the Nanog gene in nasopharyngeal carcinoma.

Key words: MiR-150, Side population cells, Invasion, Non- side population cells, Proliferation, Nasopharyngeal carcinoma

中图分类号: 

  • R739.63
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