山东大学耳鼻喉眼学报 ›› 2020, Vol. 34 ›› Issue (4): 97-100.doi: 10.6040/j.issn.1673-3770.0.2019.413

• 论著 • 上一篇    下一篇

2-APB体外抑制嗜中性粒细胞型鼻息肉中的炎症反应

戴飞,魏瑾瑾,汤欣玥,陈峥,蔺林   

  1. 戴飞, 魏瑾瑾, 汤欣玥, 陈峥, 蔺林复旦大学附属华山医院北院 耳鼻咽喉头颈外科, 上海 201907
  • 收稿日期:2019-08-19 出版日期:2020-07-20 发布日期:2020-08-28

2- aminoethoxydiphenyl borate inhibits inflammatory reactions of neutrophilic nasal polyps in vitroDAI Fei, WEI Jinjin, TANG Xinyue, CHEN Zheng, LIN Lin Department of Otorhinolaryngology-Head and Neck Surgery, Huashan Hospital North of Fudan University, Shanghai 201907, ChinaAbstract:Objective〓

Nasal polyps(NP)are frequently divided into subtypes, including eosinophilic and neutrophilic, among others, according to their endotypes. However, the mechanisms of neutrophilic NP need to be further elucidated. This study explores the influence of 2-aminoethoxydiphenyl borate(2-APB)on inflammatory reactions of neutrophilic NP in vitro. MethodsWe used immunofluorescence and immunohistochemical staining to detect the infiltration of neutrophils(NEU)in normal nasal mucosa and NP tissues. These tissues were subsequently cultured in vitro using an organ culture technique, and concentrations of Orai1 and myeloperoxidase(MPO)were determined using enzyme-linked immunosorbent assay(ELISA). 2-APB was administered to both cultures, and the measurement of Orai1 and MPO concentrations using ELISA was repeated. ResultsAll samples were identified as neutrophilic NP. The number of NEU was higher in NP than in normal nasal mucosa. Concentrations of Orai1 and MPO were also higher in NP cultured in vitro than in normal tissue. After the administration of 2-APB, Orai1 protein and the mediator MPO were reduced significantly in the cultures. Conclusion2-APB may inhibit inflammatory reactions of neutrophilic NP in vitro.   

  1. Keywords: Neutrophils;
    Nasal polyps;
    Nasal mucosa;
    Inflammatory mediator;
    Myeloperoxidase慢性鼻窦炎(chronic rhinosinusitis, CRS)临床常分为两型:慢性鼻窦炎不伴鼻息肉(chronic rhinosinusitis without nasal polyps, CRSsNP)和慢性鼻窦炎伴有鼻息肉(chronic rhinosinusitis with nasal polyps, CRSwNP)[1-2]。CRS在我国的发病率大约为8%[3], 该病会给患者本人及其家庭都带来繁重的经济负担和削弱的劳动力, 目前针对CRS的治疗已成为国家和社会的沉重负担。
  • Received:2019-08-19 Online:2020-07-20 Published:2020-08-28

摘要: 目的 鼻息肉(NP)按照其细胞内表型可分为嗜酸性粒细胞(EOS)型、嗜中性粒细胞(NEU)型等,而NEU型NP的发病机制尚不十分清楚。研究旨在探讨2-氨基乙基联苯基硼酸酯(2-APB)体外干预对NEU型NP中的炎症反应的影响。 方法 分别用免疫荧光染色方法和免疫组织化学染色方法检测正常鼻黏膜组织和NP组织中NEU的浸润情况,接着用器官培养方法将两种组织进行体外培养,随后用酶联免疫吸附试验(ELISA)检测其中Orai1蛋白和炎症介质髄过氧化物酶(MPO)的含量,然后将2-APB加入两种组织的培养基中,再次以ELISA法检测其中Orai1和MPO的浓度。 结果 所有NP标本均鉴定为NEU型,NP组织中NEU数目明显多于正常鼻黏膜组织,体外培养的NP组织中Orai1蛋白和MPO含量均显著高于正常鼻黏膜组织,2-APB干预后Orai1和炎症介质MPO浓度均出现下降。 结论 2-APB可以抑制NEU型NP的炎症反应。

关键词: 嗜中性粒细胞, 鼻息肉, 鼻黏膜, 炎症介质, 髄过氧化物酶

中图分类号: 

  • R765.4
[1] Fokkens WJ, Lund VJ, Mullol J, et al. European position paper on rhinosinusitis and nasal polyps 2012[J]. Rhinol Suppl, 2012, 23: 3 p preceding table of contents, 1-3 p preceding table of contents298.
[2] 中华耳鼻咽喉头颈外科杂志编辑委员会鼻科组, 中华医学会耳鼻咽喉头颈外科学分会鼻科学组. 中国慢性鼻窦炎诊断和治疗指南(2018)[J]. 中华耳鼻咽喉头颈外科杂志, 2019, 54(2): 81-100. doi:10.3760/cma.j.issn.1673-0860.2019.02.001. Chinese guidelines for diagnosis and treatment of chronic rhinosinusitis(2018)[J]. Chinese Journal of Otorhinolaryngology Head and Neck Surgery, 2019, 54(2): 81-100. doi:10.3760/cma.j.issn.1673-0860.2019.02.001.
[3] Shi JB, Fu QL, Zhang H, et al. Epidemiology of chronic rhinosinusitis: results from a cross-sectional survey in seven Chinese cities[J]. Allergy, 2015, 70(5): 533-539. doi:10.1111/all.12577.
[4] Zhang N, van Zele T, Perez-Novo C, et al. Different types of T-effector cells orchestrate mucosal inflammation in chronic sinus disease[J]. J Allergy Clin Immunol, 2008, 122(5): 961-968. doi:10.1016/j.jaci.2008.07.008.
[5] Mahdavinia M, Suh LA, Carter RG, et al. Increased noneosinophilic nasal polyps in chronic rhinosinusitis in US second-generation Asians suggest genetic regulation of eosinophilia[J]. J Allergy Clin Immunol, 2015, 135(2): 576-579. doi:10.1016/j.jaci.2014.08.031.
[6] Park SK, Lee WJ, Yang YI. Organ culture at the air-liquid interface maintains structural and functional integrities of inflammatory and fibrovascular cells of nasal polyps[J]. Am J Rhinol, 2007, 21(4): 402-407. doi:10.2500/ajr.2007.21.3050.
[7] Lin L, Wei JJ, Chen Z, et al. Activations of group 2 innate lymphoid cells depend on endotypes of chronic rhinosinusitis[J]. Eur Arch Otorhinolaryngol, 2018, 275(12): 3007-3016. doi:10.1007/s00405-018-5180-4.
[8] Berridge MJ, Bootman MD, Llewelyn Roderick H. Calcium signalling: dynamics, homeostasis and remodelling[J]. Nature, 2003, 4(7): 517. doi:10.1038/nrm1155.[LinkOut]
[9] Lin L, Zheng CQ, Zhang LP, et al. Up-regulation of Orai1 in murine allergic rhinitis[J]. Histochem Cell Biol, 2010, 134(1): 93-102. doi:10.1007/s00418-010-0717-7.
[10] Yeung PS, Yamashita M, Ing CE, et al. Mapping the functional anatomy of Orai1 transmembrane domains for CRAC channel gating[J]. Proc Natl Acad Sci USA, 2018, 115(22): E5193-E5202. doi:10.1073/pnas.1718373115.
[11] Hogan PG, Rao A. Store-operated calcium entry: Mechanisms and modulation[J]. Biochem Biophys Res Commun, 2015, 460(1): 40-49. doi:10.1016/j.bbrc.2015.02.110.
[12] Frischauf I, Fahrner M, Jardín I, et al. The STIM1: orai interaction[J]. Adv Exp Med Biol, 2016, 898: 25-46. doi:10.1007/978-3-319-26974-0_2.
[13] Shim AH, Tirado-Lee L, Prakriya M. Structural and functional mechanisms of CRAC channel regulation[J]. J Mol Biol, 2015, 427(1): 77-93. doi:10.1016/j.jmb.2014.09.021.
[14] Sogkas G, Vögtle T, Rau E, et al. Orai1 controls C5a-induced neutrophil recruitment in inflammation[J]. Eur J Immunol, 2015, 45(7): 2143-2153. doi:10.1002/eji.201445337.
[15] Immler R, Simon SI, Sperandio M. Calcium signalling and related Ion channels in neutrophil recruitment and function[J]. Eur J Clin Invest, 2018, 48(Suppl 2): e12964. doi:10.1111/eci.12964.
[16] Zhang N, van Zele T, Perez-Novo C, et al. Different types of T-effector cells orchestrate mucosal inflammation in chronic sinus disease[J]. J Allergy Clin Immunol, 2008, 122(5): 961-968. doi:10.1016/j.jaci.2008.07.008.
[17] Peinelt C, Lis A, Beck A, et al. 2-Aminoethoxydiphenyl borate directly facilitates and indirectly inhibits STIM1-dependent gating of CRAC channels[J]. J Physiol(Lond), 2008, 586(13): 3061-3073. doi:10.1113/jphysiol.2008.151365.
[1] 陈国辉,和守睆,孙青,高天乐,白广平. 鼻内镜术中不同中鼻甲处理方式治疗慢性鼻-鼻窦炎伴鼻息肉效果比较[J]. 山东大学耳鼻喉眼学报, 2019, 33(5): 82-86.
[2] 张宇,宋西成. 慢性鼻窦炎伴鼻息肉与哮喘的相关性机制及治疗策略研究进展[J]. 山东大学耳鼻喉眼学报, 2019, 33(1): 49-52.
[3] 王亚坤,张宇,宋西成. 不伴有下气道疾病的慢性鼻-鼻窦炎患者肺功能及相关因素分析[J]. 山东大学耳鼻喉眼学报, 2019, 33(1): 99-102.
[4] 王建伟,张宇,宋西成. 慢性鼻-鼻窦炎伴鼻息肉合并哮喘患者肺功能分析[J]. 山东大学耳鼻喉眼学报, 2019, 33(1): 103-108.
[5] 宋晓宇, 张宇, 李成林, 宋西成. 加速康复外科理念在慢性鼻-鼻窦炎伴鼻息肉手术治疗中的应用[J]. 山东大学耳鼻喉眼学报, 2018, 32(5): 9-12.
[6] 王驰,刘星,孔磊,洪兴和,宁博. 鼻内镜下等离子射频消融术对合并鼻窦炎的小儿腺样体肥大患者疗效及鼻腔黏膜纤毛清除功能的影响[J]. 山东大学耳鼻喉眼学报, 2018, 32(5): 78-81.
[7] 黄远,巴罗,李波,余文兴,杨芳丽,杜进涛. 慢性鼻窦炎鼻息肉中转录因子NKX2-1调控嗜酸性炎症反应的作用[J]. 山东大学耳鼻喉眼学报, 2018, 32(4): 17-23.
[8] 陈望兴,赵雪,刘岩,刘悦,金春顺. 鼻中隔偏曲及慢性鼻窦炎鼻息肉患者的精神心理状态调查[J]. 山东大学耳鼻喉眼学报, 2018, 32(4): 24-27.
[9] 黄凯丰. 糖皮质激素短疗程雾化吸入对嗜酸粒细胞性鼻窦炎伴鼻息肉患者鼻部症状及肾上腺皮质功能的影响[J]. 山东大学耳鼻喉眼学报, 2018, 32(4): 28-31.
[10] 李华斌, 赖玉婷. 慢性鼻-鼻窦炎的发病机制及诊疗进展[J]. 山东大学耳鼻喉眼学报, 2018, 32(3): 4-9.
[11] 李宏梅,王兰,高照慧,王莹,薛金梅. p-ERK2及ERK2在鼻息肉组织的表达及其意义[J]. 山东大学耳鼻喉眼学报, 2018, 32(3): 42-46.
[12] 甘彬,张永举,许安廷. BRF2在慢性鼻-鼻窦炎伴鼻息肉发病过程中的作用研究[J]. 山东大学耳鼻喉眼学报, 2018, 32(3): 47-53.
[13] 张劼,龚齐. 不同分型慢性鼻-鼻窦炎患者外周血嗜酸性粒细胞与中性粒细胞百分比及变态反应临床差异性分析[J]. 山东大学耳鼻喉眼学报, 2018, 32(3): 54-57.
[14] 于倩倩,关鸽,张念凯,李娜,温君凤,刘婷婷,连媛媛. 鼻腔嗅裂区呼吸道上皮腺瘤样错构瘤的CT影像表现[J]. 山东大学耳鼻喉眼学报, 2018, 32(3): 76-81.
[15] 郅莉莉,宋道亮. 嗜酸性粒细胞及IL5在上颌窦后鼻孔息肉与鼻息肉中表达的差异[J]. 山东大学耳鼻喉眼学报, 2017, 31(4): 43-46.
Viewed
Full text


Abstract

Cited

  Shared   
  Discussed   
No Suggested Reading articles found!