ISSN 1673-3770 CN 37-1437/R
山东大学耳鼻喉眼学报 ›› 2020, Vol. 34 ›› Issue (4): 97-100.doi: 10.6040/j.issn.1673-3770.0.2019.413
戴飞,魏瑾瑾,汤欣玥,陈峥,蔺林
Nasal polyps(NP)are frequently divided into subtypes, including eosinophilic and neutrophilic, among others, according to their endotypes. However, the mechanisms of neutrophilic NP need to be further elucidated. This study explores the influence of 2-aminoethoxydiphenyl borate(2-APB)on inflammatory reactions of neutrophilic NP in vitro. MethodsWe used immunofluorescence and immunohistochemical staining to detect the infiltration of neutrophils(NEU)in normal nasal mucosa and NP tissues. These tissues were subsequently cultured in vitro using an organ culture technique, and concentrations of Orai1 and myeloperoxidase(MPO)were determined using enzyme-linked immunosorbent assay(ELISA). 2-APB was administered to both cultures, and the measurement of Orai1 and MPO concentrations using ELISA was repeated. ResultsAll samples were identified as neutrophilic NP. The number of NEU was higher in NP than in normal nasal mucosa. Concentrations of Orai1 and MPO were also higher in NP cultured in vitro than in normal tissue. After the administration of 2-APB, Orai1 protein and the mediator MPO were reduced significantly in the cultures. Conclusion2-APB may inhibit inflammatory reactions of neutrophilic NP in vitro.
摘要： 目的 鼻息肉(NP)按照其细胞内表型可分为嗜酸性粒细胞(EOS)型、嗜中性粒细胞(NEU)型等,而NEU型NP的发病机制尚不十分清楚。研究旨在探讨2-氨基乙基联苯基硼酸酯(2-APB)体外干预对NEU型NP中的炎症反应的影响。 方法 分别用免疫荧光染色方法和免疫组织化学染色方法检测正常鼻黏膜组织和NP组织中NEU的浸润情况,接着用器官培养方法将两种组织进行体外培养,随后用酶联免疫吸附试验(ELISA)检测其中Orai1蛋白和炎症介质髄过氧化物酶(MPO)的含量,然后将2-APB加入两种组织的培养基中,再次以ELISA法检测其中Orai1和MPO的浓度。 结果 所有NP标本均鉴定为NEU型,NP组织中NEU数目明显多于正常鼻黏膜组织,体外培养的NP组织中Orai1蛋白和MPO含量均显著高于正常鼻黏膜组织,2-APB干预后Orai1和炎症介质MPO浓度均出现下降。 结论 2-APB可以抑制NEU型NP的炎症反应。
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