ISSN 1673-3770 CN 37-1437/R
山东大学耳鼻喉眼学报 ›› 2020, Vol. 34 ›› Issue (4): 97-100.doi: 10.6040/j.issn.1673-3770.0.2019.413
戴飞,魏瑾瑾,汤欣玥,陈峥,蔺林
Nasal polyps(NP)are frequently divided into subtypes, including eosinophilic and neutrophilic, among others, according to their endotypes. However, the mechanisms of neutrophilic NP need to be further elucidated. This study explores the influence of 2-aminoethoxydiphenyl borate(2-APB)on inflammatory reactions of neutrophilic NP in vitro. MethodsWe used immunofluorescence and immunohistochemical staining to detect the infiltration of neutrophils(NEU)in normal nasal mucosa and NP tissues. These tissues were subsequently cultured in vitro using an organ culture technique, and concentrations of Orai1 and myeloperoxidase(MPO)were determined using enzyme-linked immunosorbent assay(ELISA). 2-APB was administered to both cultures, and the measurement of Orai1 and MPO concentrations using ELISA was repeated. ResultsAll samples were identified as neutrophilic NP. The number of NEU was higher in NP than in normal nasal mucosa. Concentrations of Orai1 and MPO were also higher in NP cultured in vitro than in normal tissue. After the administration of 2-APB, Orai1 protein and the mediator MPO were reduced significantly in the cultures. Conclusion2-APB may inhibit inflammatory reactions of neutrophilic NP in vitro.
摘要： 目的 鼻息肉(NP)按照其细胞内表型可分为嗜酸性粒细胞(EOS)型、嗜中性粒细胞(NEU)型等,而NEU型NP的发病机制尚不十分清楚。研究旨在探讨2-氨基乙基联苯基硼酸酯(2-APB)体外干预对NEU型NP中的炎症反应的影响。 方法 分别用免疫荧光染色方法和免疫组织化学染色方法检测正常鼻黏膜组织和NP组织中NEU的浸润情况,接着用器官培养方法将两种组织进行体外培养,随后用酶联免疫吸附试验(ELISA)检测其中Orai1蛋白和炎症介质髄过氧化物酶(MPO)的含量,然后将2-APB加入两种组织的培养基中,再次以ELISA法检测其中Orai1和MPO的浓度。 结果 所有NP标本均鉴定为NEU型,NP组织中NEU数目明显多于正常鼻黏膜组织,体外培养的NP组织中Orai1蛋白和MPO含量均显著高于正常鼻黏膜组织,2-APB干预后Orai1和炎症介质MPO浓度均出现下降。 结论 2-APB可以抑制NEU型NP的炎症反应。
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| [1] | Fokkens WJ, Lund VJ, Mullol J, et al. European position paper on rhinosinusitis and nasal polyps 2012[J]. Rhinol Suppl, 2012, 23: 3 p preceding table of contents, 1-3 p preceding table of contents298. |
| [2] | 中华耳鼻咽喉头颈外科杂志编辑委员会鼻科组, 中华医学会耳鼻咽喉头颈外科学分会鼻科学组. 中国慢性鼻窦炎诊断和治疗指南(2018)[J]. 中华耳鼻咽喉头颈外科杂志, 2019, 54(2): 81-100. doi:10.3760/cma.j.issn.1673-0860.2019.02.001. Chinese guidelines for diagnosis and treatment of chronic rhinosinusitis(2018)[J]. Chinese Journal of Otorhinolaryngology Head and Neck Surgery, 2019, 54(2): 81-100. doi:10.3760/cma.j.issn.1673-0860.2019.02.001. |
| [3] | Shi JB, Fu QL, Zhang H, et al. Epidemiology of chronic rhinosinusitis: results from a cross-sectional survey in seven Chinese cities[J]. Allergy, 2015, 70(5): 533-539. doi:10.1111/all.12577. |
| [4] | Zhang N, van Zele T, Perez-Novo C, et al. Different types of T-effector cells orchestrate mucosal inflammation in chronic sinus disease[J]. J Allergy Clin Immunol, 2008, 122(5): 961-968. doi:10.1016/j.jaci.2008.07.008. |
| [5] | Mahdavinia M, Suh LA, Carter RG, et al. Increased noneosinophilic nasal polyps in chronic rhinosinusitis in US second-generation Asians suggest genetic regulation of eosinophilia[J]. J Allergy Clin Immunol, 2015, 135(2): 576-579. doi:10.1016/j.jaci.2014.08.031. |
| [6] | Park SK, Lee WJ, Yang YI. Organ culture at the air-liquid interface maintains structural and functional integrities of inflammatory and fibrovascular cells of nasal polyps[J]. Am J Rhinol, 2007, 21(4): 402-407. doi:10.2500/ajr.2007.21.3050. |
| [7] | Lin L, Wei JJ, Chen Z, et al. Activations of group 2 innate lymphoid cells depend on endotypes of chronic rhinosinusitis[J]. Eur Arch Otorhinolaryngol, 2018, 275(12): 3007-3016. doi:10.1007/s00405-018-5180-4. |
| [8] | Berridge MJ, Bootman MD, Llewelyn Roderick H. Calcium signalling: dynamics, homeostasis and remodelling[J]. Nature, 2003, 4(7): 517. doi:10.1038/nrm1155.[LinkOut] |
| [9] | Lin L, Zheng CQ, Zhang LP, et al. Up-regulation of Orai1 in murine allergic rhinitis[J]. Histochem Cell Biol, 2010, 134(1): 93-102. doi:10.1007/s00418-010-0717-7. |
| [10] | Yeung PS, Yamashita M, Ing CE, et al. Mapping the functional anatomy of Orai1 transmembrane domains for CRAC channel gating[J]. Proc Natl Acad Sci USA, 2018, 115(22): E5193-E5202. doi:10.1073/pnas.1718373115. |
| [11] | Hogan PG, Rao A. Store-operated calcium entry: Mechanisms and modulation[J]. Biochem Biophys Res Commun, 2015, 460(1): 40-49. doi:10.1016/j.bbrc.2015.02.110. |
| [12] | Frischauf I, Fahrner M, Jardín I, et al. The STIM1: orai interaction[J]. Adv Exp Med Biol, 2016, 898: 25-46. doi:10.1007/978-3-319-26974-0_2. |
| [13] | Shim AH, Tirado-Lee L, Prakriya M. Structural and functional mechanisms of CRAC channel regulation[J]. J Mol Biol, 2015, 427(1): 77-93. doi:10.1016/j.jmb.2014.09.021. |
| [14] | Sogkas G, Vögtle T, Rau E, et al. Orai1 controls C5a-induced neutrophil recruitment in inflammation[J]. Eur J Immunol, 2015, 45(7): 2143-2153. doi:10.1002/eji.201445337. |
| [15] | Immler R, Simon SI, Sperandio M. Calcium signalling and related Ion channels in neutrophil recruitment and function[J]. Eur J Clin Invest, 2018, 48(Suppl 2): e12964. doi:10.1111/eci.12964. |
| [16] | Zhang N, van Zele T, Perez-Novo C, et al. Different types of T-effector cells orchestrate mucosal inflammation in chronic sinus disease[J]. J Allergy Clin Immunol, 2008, 122(5): 961-968. doi:10.1016/j.jaci.2008.07.008. |
| [17] | Peinelt C, Lis A, Beck A, et al. 2-Aminoethoxydiphenyl borate directly facilitates and indirectly inhibits STIM1-dependent gating of CRAC channels[J]. J Physiol(Lond), 2008, 586(13): 3061-3073. doi:10.1113/jphysiol.2008.151365. |
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