KCNK5双孔钾通道蛋白在甲状腺乳头状癌中的表达及临床意义

doi:10.6040/j.issn.1673-3770.0.2021.004

摘要/Abstract

摘要： 目的检测KCNK5在甲状腺乳头状癌(PTC)组织中的表达,探讨其与临床病理特征及患者预后的相关性。方法应用免疫组织化学和Western bloting方法检测PTC组织及癌旁正常组织中KCNK5表达,分析其与PTC临床病理特征的关系,KM Plotter数据库分析KCNK5与PTC预后的关系。结果免疫组化结果显示KCNK5表达于PTC细胞的胞膜、核膜和胞质,癌旁正常组织阴性或弱阳性;Western blot和免疫组化半定量积分显示PTC组织中的KCNK5水平显著高于癌旁正常组织(P<0.05)。KCNK5表达与PTC患者的TNM分期、淋巴结转移密切相关(均P<0.05);多因素Logistic回归分析进一步证实KCNK5与淋巴结转移密切相关(P<0.05)。Kaplan-Meier分析显示KCNK5高表达患者生存率明显低于KCNK5低表达患者(P<0.01)。结论 KCNK5与PTC患者淋巴结转移和预后密切相关,有望成为PTC评估预后的指标。

关键词: 甲状腺乳头状癌, 双孔钾通道, KCNK5, 免疫组织化学, Western bloting方法, KM Plotter数据库

Abstract: Objective To detect the expression of KCNK5 in papillary thyroid carcinoma(PTC)tissues and investigate its correlation with clinicopathological characteristics and prognosis in patients with PTC. Methods Immunohistochemistry and western blotting were used to detect the expression of KCNK5 in PTC tissues and paraneoplastic normal tissues, and the relationship between KCNK5 and the clinicopathological characteristics of PTC was analyzed; moreover, the relationship between KCNK5 and the prognosis of PTC was analyzed using the KM Plotter database. Results Immunohistochemical results showed that KCNK5 was expressed in the cytosol, nuclear membrane, and cytoplasm of PTC cells, and KCNK5 was either not expressed or weakly positively expressed in paraneoplastic normal tissues. Western blotting and immunohistochemical semi-quantitative integration showed that the expression level of KCNK5 in PTC tissues was significantly higher than that in paraneoplastic normal tissues(P<0.05). KCNK5 expression was positively correlated with TNM stage and lymph node metastasis in PTC patients(both P<0.05); multivariate logistic regression analysis further indicated that KCNK5 expression was related to lymph node metastasis(P<0.05). Kaplan-Meier analysis showed that the survival rate of patients with a high KCNK5 expression was significantly lower than that of patients with a low KCNK5 expression(P<0.01). Conclusion KCNK5 is closely associated with lymph node metastasis and prognosis of PTC patients and has the potential to become an indicator of PTC prognosis.

Key words: Papillary thyroid carcinoma, Two pore domain K⁺ channel, KCNK5, Immunohistochemistry, Western bloting, KM Plotter

中图分类号:

R736.1

薛刚,孙新民,林旭,吴靖芳. KCNK5双孔钾通道蛋白在甲状腺乳头状癌中的表达及临床意义[J]. 山东大学耳鼻喉眼学报, 2021, 35(4): 64-69.

XUE Gang, SUN Xinmin, LIN Xu, WU Jingfang. Expression and clinical significance of the KCNK5 double-pore potassium channel protein in papillary thyroid carcinoma[J]. Journal of Otolaryngology and Ophthalmology of Shandong University, 2021, 35(4): 64-69.

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