山东大学耳鼻喉眼学报 ›› 2021, Vol. 35 ›› Issue (5): 125-131.doi: 10.6040/j.issn.1673-3770.0.2020.449

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感染性角膜炎的模型制备

闫语1,2,曾澳1,2，何宇茜1   

  1. 1.吉林大学第二医院 眼科中心, 吉林 长春 130041;
    2.吉林大学白求恩医学部, 吉林 长春 130021
  • 发布日期:2021-09-29
  • 通讯作者: 何宇茜. E-mall:heyuxihot@163.com
  • 基金资助:
    吉林省科技厅国际科技合作项目(20200801016GH);吉林省医疗卫生人才专项项目(2019SCZT052,2019SCZT057);吉林大学优秀青年教师培养计划(419080500586,419080520252);吉林省科技厅自然科学基金(20180101146JC);吉林省财政厅青年人才培养项目(3D5197389429);吉林省中医药科技资助项目(2019134);BMP4抑制角膜新生血管的机制研究(3R2196623429);专业学位研究生课程案例库(419100200379);吉林省科技厅国际科技合作项目(20180414063GH);吉林省教育厅科学技术研究项目(JJKH20201103KJ);吉林大学基本科研业务费项目(45119031C004);吉林省卫生专项项目(2020SCZT089)

Model preparation of infectious keratitis

YAN Yu1,2, ZENG Ao1,2,HE Yuxi1   

  1. 1. Department of Ophthalmology, the Second Hospital of Jilin University, Changchun 130041, Jilin, China;
    2. Norman Bethune Health Science Center of Jilin University, Changchun 130021, Jilin, China
  • Published:2021-09-29

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摘要： 感染性角膜炎是一种由致病微生物侵袭角膜组织引起的炎症,是世界性的常见致盲眼病,主要致病的病原体有细菌、真菌、病毒和棘阿米巴。其中,细菌感染是感染性角膜炎的主要原因,但近年来真菌性角膜炎有逐年增多趋势。目前的有效治疗手段主要是根据致病微生物不同,以局部使用或结膜下注射抗细菌和真菌制剂及全身运用抗病毒药物为主。综述从菌液制备、操作方法到评分检测方法系统地阐述了由细菌、真菌、病毒和棘阿米巴引起的角膜炎的模型制备。就如何制备出具有针对性的动物模型进行探讨,为评价及发掘治疗感染性角膜炎的治疗效果和不良反应提供依据。

关键词: 感染性角膜炎, 动物模型, 真菌性角膜炎, 细菌性角膜炎

Abstract: Infectious keratitis is an inflammation caused by the invasion of pathogenic microorganisms on corneal tissue. It is a common cause of blindness in the world. The main pathogenic microorganisms are bacteria, fungi, viruses, and Acanthamoeba. Bacteria is the main cause of infectious keratitis, but fungal keratitis has been increasing year by year in recent years. At present, the effective treatment is local use or subconjunctival injection of anti-bacterial and fungal agents, and systemic use of antiviral drugs, based on the different pathogenic microorganisms. We systematically describe models preparation of infectious keratitis caused by bacteria, fungi, viruses, and Acanthamoeba, including the preparation of the bacterial solution, operation methods and scoring detection methods. This paper elaborates how to prepare a targeted animal model to provide theoretical basis for evaluating and exploring the treatment effect and adverse reactions of infectious keratitis.

Key words: Infectious keratitis, Animal model, Fungal keratitis, Bacterial keratitis

中图分类号: 

  • R772.21
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