山东大学耳鼻喉眼学报 ›› 2010, Vol. 24 ›› Issue (01): 61-64.

• 论文 • 上一篇    下一篇

热凝脑膜中动脉对硝酸甘油致偏头痛大鼠三叉神经尾核c-fos基因表达的影响

朱晓凤1,韩月臣1,熊文萍1,逯素梅2,李建峰2,王海波1,2,樊兆民1   

  1. 1. 山东大学附属省立医院耳鼻咽喉-头颈外科,济南 250021;
    2. 山东省临床医学研究院眼耳鼻喉科学研究所,济南 250021
  • 收稿日期:2009-11-17 修回日期:2010-01-17 出版日期:2010-02-16 发布日期:2010-02-16
  • 通讯作者: 樊兆民(1956- ),男,教授,硕士生导师,主要从事耳科疾病的临床研究
  • 作者简介:朱晓凤(1985- ),女,硕士研究生,主要从事耳鼻咽喉科学的临床与基础研究工作。 Email:zhuxiaofeng.1984@163.com
  • 基金资助:

    山东省科技攻关计划课题(2007GG20002)

Effects of heating coagulation of the middle meningeal artery on c-fos expression in the trigeminal  caudalis nucleus in migrainerats triggered by nitroglycerin

ZHU Xiaofeng1, HAN Yuechen1, XIONG Wenping1, LU Sumei2, LI Jianfeng2,WANG Haibo1,2, FAN Zhaomin1   

  1. 1. OtolaryngologyHead and Neck Surgery, Provincial Hospital Affiliated to Shandong University, Jinan 250021,China;
    2. Institute of Eye and ENT, Shandong  Clinic Research Institute, Jinan 250021,, China
  • Received:2009-11-17 Revised:2010-01-17 Online:2010-02-16 Published:2010-02-16

摘要:

目的  探讨热凝脑膜中动脉对硝酸甘油诱发的偏头痛大鼠三叉神经尾核c-fos基因表达的影响。方法  48只健康雌性Wistar大鼠随机分为4组(每组12只):生理盐水组(A组),硝酸甘油组(B组,10mg/kg),假手术组(C组),手术组(D组)。观察各组大鼠行为学表现,成功建模后每组随即平分为2小组分别采用RT-PCR和Western blot法检测大鼠三叉神经尾核c-fos基因表达。结果  各组大鼠挠头、爬笼及耳红等行为学评分。结论  热凝脑膜中动脉可显著减轻硝酸甘油诱发的大鼠偏头痛症状,表明脑膜中动脉在硝酸甘油诱导的大鼠偏头痛模型致痛过程中起主要作用,这可能是其通过降低硝酸甘油诱导的大鼠三叉神经尾核c-fos基因表达,减少或抑制三叉神经尾核神经元的激活引起的。

关键词: 偏头痛; 脑膜中动脉;三叉神经尾核;c-fos基因;大鼠,Wistar

Abstract:

Objective  To detect the influence of heating coagulation of the middle meningeal artery on c-fos expression in the trigeminal  caudalis nucleus in migraine rats triggered by nitroglycerin. Methods  Forty-eight female Wistar rats were randomly assigned to four groups, the isotonic saline group (group A), the nitroglycerin group (group B), the operation group (group C), and the sham operation group (group D), with 12 subjects in each group and each group was subdivided into another two equal groups after successfully constructing  the animal model. C-fos expression in the trigeminal caudalis nucleus  was detected by means of  RT-PCR and  Western blot respectively. Results  Injection of 10mg/kg nitroglycerin was able to trigger the animal model of migraine as evidenced by the appearances of a series of symptoms, such as   frequently head scratching, climbing the cage and  red ear. The scores of these behaviors for each group were respectively 2.33±0.74, 39.57±5.44, 37.87±4.68, 15.6±3.30. At the same time,  c-fos expressionsin the trigeminal caudalis nucleu  in group B, group C, or group D was significantly increased  compared with that of group A (P<0.05). More importantly,  c-fos expression in the trigeminal caudalis  nucleus in group D was much lower than that in group B and group C (P<0.05), but there was no statistical difference between group B and group C (P>0.05). Conclusions  Results show that heat coagulation of the middle meningeal artery may attenuate head complaint of rats induced by nitroglycerin,  and it is concluded that the middle meningeal artery plays a primary role in the pathophysiology of the rat migraine model triggered by nitroglycerin and the efficacy may play a part in decreasing c-fos expression and containing activation of neurons in the trigeminal caudalis nucleus .

Key words: Migraine; Middle meningeal artery; Trigeminal  caudalis nucleus; Cfos Genes; Rats,Wistar

中图分类号: 

  • R747.2
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