JOURNAL OF SHANDONG UNIVERSITY (OTOLARYNGOLOGY AND OPHTHALMOLOGY) ›› 2015, Vol. 29 ›› Issue (5): 38-42.doi: 10.6040/j.issn.1673-3770.0.2015.143

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Effects of silencing hypoxia inducible factor-1α on the expression of cytokines in cultured human nasal epithelial cells

LI Yingying, ZHOU Han, ZHANG Weiqiang, DONG Weida   

  1. Department of Otorhinolaryngology, The First Affiliated Hospital with Nanjing Medical University, Jiangsu Provence Hospital, Nanjing 210029, Jiangsu, China
  • Received:2015-04-07 Revised:2015-07-09 Online:2015-10-16 Published:2015-10-16

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Abstract: Objective To investigate the effects of silencing hypoxia inducible factor-1α(HIF-1α) by transfecting short hairpin RNA(shRNA)into cultured human nasal epithelial cells (HNECs) and its further regulations on vascular endothelial growth factor(VEGF), transforming growth factor(TGF)β1 and basic fibroblast growth factor(bFGF). Methods HNECs were isolated from 10 inferior turbinate-cutting patients'mucosa. Two HIF-shRNA fragments were built and the best silencing shRNA sequence was used for transfection. We separated cells into three groups: the blank group, the negative control group and the ad-HIFshRNA interference group. The protein and gene expressions were detected by Western blotting and PCR. Results HNECs were transfected with GFP marketed adenovirus which carry the best silencing HIF-1α shRNA. The protein and mRNA expressions of HIF-1α were significantly reduced 40% and 39%(q=31.469, 16.590), and following, the protein and mRNA expressions of VEGF, TGF-β1 and bFGF were reduced (P<0.05). Conclusion Transfection of ad-HIFshRNA can effectively silence the expression of HIF-1α gene and protein and furthermore inhibit the expressions of VEGF, TGF-β1 and FGF-2, which suggests HIF-1α regulates the expression of VEGF, bFGF and TGF-β1 through direct or indirect ways. Thus silencing HIF-1α may provide a new way towards allergic rhiniti's treatment.

Key words: Nasal epithilial cells, RNA interference, Hypoxia-inducible factor-1α, Transforming growth factor-β1, Vascular endothelial growth factor, Basic fibroblast growth factor

CLC Number: 

  • R765.2
[1] Baldea A J, Luchette F A. The role of hypoxia-inducible factor-1alpha in response to injury and hypoxia[J]. Crit Care Med, 2014, 42(10):2312-2313.
[2] Walmsley S R, Cadwallader K A, Chilvers E R. The role of HIF-1alpha in myeloid cell inflammation[J]. Trends Immunol, 2005, 26(8):434-439.
[3] Bollinger T, Gies S, Naujoks J, et al. HIF-1alpha-and hypoxia-dependent immune responses in human CD4+CD25high T cells and T helper 17 cells[J]. J Leukoc Biol, 2014, 96(2):305-312.
[4] Hirota S A, Beck P L, MacDonald J A. Targeting hypoxia-inducible factor-1 (HIF-1) signaling in therapeutics: implications for the treatment of inflammatory bowel disease[J]. Recent Pat Inflamm Allergy Drug Discov, 2009, 3(1):1-16.
[5] Sumbayev V V, Yasinska I, Oniku A E, et al. Involvement of hypoxia-inducible factor-1 in the inflammatory responses of human LAD2 mast cells and basophils[J]. PloS One,2012, 7(3):e34259.
[6] Steinke J W, Woodard C R, Borish L. Role of hypoxia in inflammatory upper airway disease[J]. Curr Opin Allergy Clin Immunol, 2008, 8(1):16-20.
[7] Sumbayev V V, Nicholas S A. Hypoxia-inducible factor 1 as one of the "signaling drivers" of Toll-like receptor-dependent and allergic inflammation[J]. Arch Immunol Ther Exp (Warsz), 2010, 58(4):287-294.
[8] 杨琳红,董震.人鼻黏膜上皮细胞HIF-1α和VEGF的表达及意义[J].临床耳鼻咽喉头颈外科杂志, 2008, 22(8):341-345.YANG Linhong, DONG Zhen. Expression and the role of HIF-la and VEGF in human nasal epithelial cells[J]. J Clin Otolaryngol Head Neck Surgery, 2008, 22(8):341-345.
[9] Kim C H, Kim J K, Kim H J, et al. Comparison of intranasal ciclesonide, oral levocetirizine,and combination treatment for allergic rhinitis[J]. Allergy Asthma Immunol Res, 2015, 7(2):158-166.
[10] Meadows A, Kaambwa B, Novielli N, et al. A systematic review and economic evaluation of subcutaneous and sublingual allergen immunotherapy in adults and children with seasonal allergic rhinitis[J]. Health Technol Assess [JP3](Winchester, England), 2013, 17(27):vi, xi-xiv, 1-322.
[11] Baay-Guzman G J, Bebenek I G, Zeidler M, et al. HIF-1 expression is associated with CCL2 chemokine expression in airway inflammatory cells: implications in allergic airway inflammation[J]. Respirat Res,2012,13(1):60.
[12] Huerta-Yepez S, Baay-Guzman G J, Bebenek I G, et al. Hypoxia inducible factor promotes murine allergic airway inflammation and is increased in asthma and rhinitis[J]. Allergy, 2011, 66(7):909-918.
[13] Kim S R, Lee K S, Park H S, et al.HIF-1alpha inhibition ameliorates an allergic airway disease via VEGF suppression in bronchial epithelium[J]. Eur J Immunol, 2010, 40(10):2858-2869.
[14] Zhou H, Chen X, Zhang W M, et al.HIF-1alpha inhibition reduces nasal inflammation in a murine allergic rhinitis model[J]. PloS One, 2012, 7(11):e48618.
[15] Lee K S, Kim S R, Park S J, et al. Mast cells can mediate vascular permeability through regulation of the PI3K-HIF-1alpha-VEGF axis[J]. Am J Respirat Crit Care Med, 2008, 178(8):787-797.
[16] Mo J H, Kim J H, Lim D J, et al. The role of hypoxia-inducible factor 1alpha in allergic rhinitis[J]. Am J Rhinol Allergy, 2014, 28(2):e100-106.
[17] Park S J, Lee K S, Kim S R, et al. AMPK activation reduces vascular permeability and airway inflammation by regulating HIF/VEGFA pathway in a murine model of toluene diisocyanate-induced asthma[J]. Inflamm Res, 2012, 61(10):1069-1083.
[18] Capetandes A, Zhuang M, Haque F N, et al. Vascular endothelial growth factor is increased by human pulmonary cells stimulated with Dermatophagoides sp. extract[J]. Allergy Asthma Proc, 2007, 28(3):324-330.
[19] Kim S R, Lee K S, Lee K B, et al. Recombinant IGFBP-3 inhibits allergic lung inflammation, VEGF production, and vascular leak in a mouse model of asthma[J]. Allergy, 2012, 67(7):869-877.
[20] Ganesan P, Matsubara K, Sugawara T, et al. Marine algal carotenoids inhibit angiogenesis by down-regulating FGF-2-mediated intracellular signals in vascular endothelial cells[J]. Mol Cell Biochem, 2013, 380(1-2):1-9.
[21] Yum H Y, Cho J Y, Miller M, et al. Allergen-induced coexpression of bFGF and TGF-beta1 by macrophages in a mouse model of airway remodeling: bFGF induces macrophage TGF-beta1 expression in vitro[J]. Inter Arch Allergy Immunol, 2011, 155(1):12-22.
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