JOURNAL OF SHANDONG UNIVERSITY (OTOLARYNGOLOGY AND OPHTHALMOLOGY) ›› 2015, Vol. 29 ›› Issue (5): 38-42.doi: 10.6040/j.issn.1673-3770.0.2015.143

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Effects of silencing hypoxia inducible factor-1α on the expression of cytokines in cultured human nasal epithelial cells

LI Yingying, ZHOU Han, ZHANG Weiqiang, DONG Weida   

  1. Department of Otorhinolaryngology, The First Affiliated Hospital with Nanjing Medical University, Jiangsu Provence Hospital, Nanjing 210029, Jiangsu, China
  • Received:2015-04-07 Revised:2015-07-09 Online:2015-10-16 Published:2015-10-16

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Abstract: Objective To investigate the effects of silencing hypoxia inducible factor-1α(HIF-1α) by transfecting short hairpin RNA(shRNA)into cultured human nasal epithelial cells (HNECs) and its further regulations on vascular endothelial growth factor(VEGF), transforming growth factor(TGF)β1 and basic fibroblast growth factor(bFGF). Methods HNECs were isolated from 10 inferior turbinate-cutting patients'mucosa. Two HIF-shRNA fragments were built and the best silencing shRNA sequence was used for transfection. We separated cells into three groups: the blank group, the negative control group and the ad-HIFshRNA interference group. The protein and gene expressions were detected by Western blotting and PCR. Results HNECs were transfected with GFP marketed adenovirus which carry the best silencing HIF-1α shRNA. The protein and mRNA expressions of HIF-1α were significantly reduced 40% and 39%(q=31.469, 16.590), and following, the protein and mRNA expressions of VEGF, TGF-β1 and bFGF were reduced (P<0.05). Conclusion Transfection of ad-HIFshRNA can effectively silence the expression of HIF-1α gene and protein and furthermore inhibit the expressions of VEGF, TGF-β1 and FGF-2, which suggests HIF-1α regulates the expression of VEGF, bFGF and TGF-β1 through direct or indirect ways. Thus silencing HIF-1α may provide a new way towards allergic rhiniti's treatment.

Key words: Nasal epithilial cells, RNA interference, Hypoxia-inducible factor-1α, Transforming growth factor-β1, Vascular endothelial growth factor, Basic fibroblast growth factor

CLC Number: 

  • R765.2
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