JOURNAL OF SHANDONG UNIVERSITY (OTOLARYNGOLOGY AND OPHTHALMOLOGY) ›› 2016, Vol. 30 ›› Issue (1): 31-35.doi: 10.6040/j.issn.1673-3770.0.2015.318

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Effect of NLRP3 inflammasome in the pathogenesis and relapse of eosinophilic nasal polyps.

DU Zhihong1, YU Yafeng2.   

  1. 1. Department of Otorhinolaryngology, Gaochun Peoples Hospital, Nanjing 211300, Jiangsu, China;2. Department of Otorhinolaryngology, The First Affiliated Hospital of Soochow University, Suzhou 215006, Jiangsu, China
  • Received:2015-08-02 Online:2016-02-16 Published:2016-02-16

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Abstract: Objective To explore the possible effect and mechanism of NLRP3 inflammasome in the pathogenesis and relapse of eosinophilic nasal polyps(ENP). Methods According to HE staining, the nasal polyp tissues from 80 cases of ENP(ENP group)and 24 cases of recurrent essinophilic nasal polyps(RENP, RENP group)as well as middle turbinate mucosa from 30 cases of deviated nasal septum(control group)were selected. The number of eosinophil(EOS)infiltration in all patients was detected and observed by HE staining. The immunohistochemistry was used to detect the expression of NLRP3 inflammasome, IL-1β and IL-18 in three groups, and the correlation between NLRP3 inflammasome and the number of EOS infiltration was analyzed in ENP and RENP groups. Results The expression of IL-1β and IL-18 in both ENP and RENP groups was higher than in control group(P<0.05), but no significant difference was presented between ENP group and RENP group(P>0.05). The expression of NLRP3 inflammasome in RENP group was higher significantly than in ENP group and control group(P<0.01), and the number of EOS infiltration more than ENP group(P<0.05)and control group(P<0.01). The expression of NLRP3 inflammasome in both ENP and RENP groups positively correlated with the number of EOS infiltration. Conclusion NLRP3 inflammasome, IL-1β and IL-18 were all associated with the pathogenesis of ENP closely, and NLRP3 inflammasome may involve in the relapse of ENP.

Key words: Eosinophil, NLRP3 inflammasome, Nasal polyps, Recurrent

CLC Number: 

  • R765.2
[1] Bteman N D, Fahy C, Woolford T J. Nasal polyps: still more questions than answers[J]. J Laryngol Otol, 2003, 117(1): 1-9.
[2] Mygind N. Nasal Allergy[M]. 2nd edition. London: Blackwell Scientific Publications, 1979: 233-234.
[3] Ishitoya J, Sakuma Y, Tsukuda M. Eosinophilic chronic rhinosinusitis in Japan[J]. Allergol Int, 2010, 59(3): 239-245.
[4] Ferguson B J. Categorization of eosinophilic chronic rhinosinusitis[J]. Curr Opin Otolaryngol Head Neck Surg, 2004, 12(3): 237-242.
[5] Sun D I, Joo Y H, Kang J M. Clinical significance of eosinophilic cationic protein levels in nasal secretions of patients with nasal polyposis[J]. Eur Arch Otorhinolaryngol, 2009, 266(7): 981-986.
[6] Martinon F, Burns K, Tschopp J. The inflammasome: a molecular platform triggering activation of inflammatory caspases and processing of pro-IL-beta[J]. Mol Cell, 2002, 10(2): 417-426.
[7] 王金风,刘晓菊,曾晓丽.NLRP3炎性小体与肺部疾病[J].国际呼吸杂志,2014,34(2):115-118.
[8] Cao P P, Li H B, Wang B F, et al. Distinct immunopathologic characteristics of various types of chronic rhino sinusitis in adult Chinese[J]. J Allergy Clin Immunol, 2009, 124(3): 478-484.
[9] Bachert C, Watelet J B, Gevaert P, et al. Pharmacological management of nasal polyposis[J]. Drugs, 2005, 65(11): 1537-1552.
[10] Albu S, Tomescu E, Mexca Z, et al. Recurrence rates in endonasal surgery for polyposis[J]. Acta Otorhinolaryngol Belg, 2004, 58(1): 79-86.
[11] Stamberger H. Diffuse eosinophil-dominated polyposis. FESS-endoscopic diagnosis and surgery of the paranasal sinuses and anterior skullbase[M]. Tuttlinge: Verlag Endo-Press, 1988: 41-44.
[12] Newman L J, Platts-Mills T A, Phillips D, et al. Chronic sinusitis: relationship of computed tomographic findings to allergy, asthma, and eosinophilia[J]. JAMA, 1994, 271(5): 363-367.
[13] Medzliitov R, Janeway C J. Innate immune recognition: mechanisms and pathways[J]. Immunol Rev, 2000, 173: 89-97.
[14] Yamasaki K, Muto J, Taylor K R, et al. NLRP3/cryopyrin is necessary for interleukin-1beta(IL-1beta)release in response to hyaluronan, an endogenous trigger of inflammation in response to injury[J]. J Biol Chem, 2009, 284(19): 12762-12771.
[15] Feldmann J, Prieur A M, Quartier P, et al. Chronic inflatile neurological cutaneous and articular syndrome is caused by mutations in CIAS1, a gene highly expressed in polymorphonuclear cells and chondrocytes[J]. Am J Hum Genet, 2002, 71(1): 198-203.
[16] Zaki M H, Boyd K L, Vogel P, et al. The NLRP3 inflammasome protects against loss of epithelial integrity and mortality during experimental colitis[J]. Immunity, 2010, 32(3): 379-391.
[17] Gauer S, Sichler O, Obermuller N, et al. IL-18 is expressed in the intercalated cell of human kidney[J]. Kidney Int, 2007, 72(9): 1081-1087.
[18] Besnard A G, Guillou N, Tschopp J, et al. NLRP3 inflammasome is required in murine asthma in the absence of aluminum adjuvant[J]. Allergy, 2011, 187(7): 1047-1057.
[19] Månsson A, Bogefors J, Cervin A, et al. NOD-like receptors in the human upper airways: a potential role in nasal polyposis[J]. Allergy, 2011, 66(5): 621-628.
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