J Otolaryngol Ophthalmol Shandong Univ ›› 2018, Vol. 32 ›› Issue (4): 17-23.doi: 10.6040/j.issn.1673-3770.0.2018.183

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Transcription factor NKX2-1 regulates eosinophilic inflammation in chronic rhinosinusitis with nasal polyps

HUANG Yuan1, BA Luo2, LI Bo3, YU Wenxing1, YANG Fangli2, DU Jintao3   

  1. Department of Otorhinolaryngology, Tibet Autonomous Region Peoples Hospital, Lhasa 850000, Tibet, China;3. Department of Otolaryngology Head and Neck Surgery, West China Hospital of Sichuan University, Chengdu 610041, Sichuan, China
  • Received:2018-04-16 Online:2018-07-20 Published:2018-07-20

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Abstract: Objective To define the endotypes of chronic rhinosinusitis with nasal polyps(CRSwNP)on the basis of the expression of different key cytokines, and to investigate the mechanism via which the new transcription factor, NKX2-1, orchestrates eosinophilic inflammation in nasal polyps(NPs). Methods Polyp tissue was obtained from patients with nasal polyps and turbinate tissue was obtained from the controls after functional nasal endoscopic surgery. The expression levels of key cytokines(IL-5, IFN-γ, and IL-17A,)as well as NKX2-1 and CCL17 in NPs of different endotypes and control tissues were determined using enzyme-linked immunosorbent assay,immunohistochemistry, and western blot analysis. Results We observed that 24% patients with CRSwNP were IL-5 positive and showed infiltration of eosinophilic cells and increased IL-4 and IgE levels; 40% NPs were negative for key cytokines(KCNNPs)but showed increased levels of IL-8 and IL-1β and no eosinophilic inflammation. The expression of NKX2-1 in IL-5 + NPs was significantly lower than in normal controls. Moreover, the expression of CCL17 was significantly higher in IL-5 + NPs, which was associated with reduced NKX2-1 expression and eosinophil accumulation. Conclusion The downregulation of NKX2-1 in IL-5+ NPs may be associated with tissue eosinophilic inflammation. This indicates that NKX2-1 might be a special factor in NP pathophysiology.

Key words: Cytokine, NKX2-1, Nasal polyps, Endotype

CLC Number: 

  • R765.4
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