大气污染中气态污染物致大鼠喉黏膜急性损伤的研究

doi:10.6040/j.issn.1673-3770.0.2019.048

Abstract

Abstract: Objective To investigate air pollutant-induced acute laryngeal mucosa injury and its possible mechanisms in rats. Methods Twelve healthy SPF Sprague Dawley(SD)rats were randomly and equally divided into control and gas pollutant exposure groups(6 rats in each group). The rats in the control group were fed in an SPF environment, while the rats in the exposure group were exposed to HEPA membrane-filtered air obtained during the red alert period in Beijing in 2016. The rats were sacrificed after exposure to pollutants for 6 days. The pathological changes in rat laryngeal mucosa and IL-1beta, IL-4, IL-5, IL-6, IL-10, IL-12, IL-13, IL-17a, IFN-gamma, and TNF-alpha cytokine expression were then detected. Results The laryngeal mucosa epithelium of rats exposed to air pollutants showed hyperkeratosis and basal cell disorder of varying degrees. IL-1beta, IL-10, IL-13, IL-17a, and TNF-alpha expression also increased (P<0.05) and IL-12 and IFN-gamma expression decreased (P<0.05) in rats exposed to air pollutants when compared to the control group. Conclusion Gas pollutants in the air can cause laryngeal mucosa injury in rats, and its mechanism may be related to Th1/Th2 imbalance, which is a Th2 dominant response.

Key words: Air pollution, Gaseous pollutants, Laryngeal mucosa, Cytokines, Acute injury

CLC Number:

R767.8

LI Xin, WU Wei, WANG Gang, SI Shaoyan, HAN Haolun, LI Baowei, FAN Xin, ZHANG Chi,. Acute laryngeal mucosa injury in rats induced by gaseous pollutants in the air[J].Journal of Otolaryngology and Ophthalmology of Shandong University, 2019, 33(6): 75-79.

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