Journal of Otolaryngology and Ophthalmology of Shandong University ›› 2019, Vol. 33 ›› Issue (6): 75-79.doi: 10.6040/j.issn.1673-3770.0.2019.048

Previous Articles    

Acute laryngeal mucosa injury in rats induced by gaseous pollutants in the air

LI Xin1,2,3, WU Wei1,2,3, WANG Gang2,3, SI Shaoyan2,3,4, HAN Haolun2,3, LI Baowei2,3, FAN Xin1,2,3, ZHANG Chi1,2,3,   

  1. SHANG Ying2, 3, DING Ruiying2, 3
  • Published:2019-12-23

Abstract: Objective To investigate air pollutant-induced acute laryngeal mucosa injury and its possible mechanisms in rats. Methods Twelve healthy SPF Sprague Dawley(SD)rats were randomly and equally divided into control and gas pollutant exposure groups(6 rats in each group). The rats in the control group were fed in an SPF environment, while the rats in the exposure group were exposed to HEPA membrane-filtered air obtained during the red alert period in Beijing in 2016. The rats were sacrificed after exposure to pollutants for 6 days. The pathological changes in rat laryngeal mucosa and IL-1beta, IL-4, IL-5, IL-6, IL-10, IL-12, IL-13, IL-17a, IFN-gamma, and TNF-alpha cytokine expression were then detected. Results The laryngeal mucosa epithelium of rats exposed to air pollutants showed hyperkeratosis and basal cell disorder of varying degrees. IL-1beta, IL-10, IL-13, IL-17a, and TNF-alpha expression also increased (P<0.05) and IL-12 and IFN-gamma expression decreased (P<0.05) in rats exposed to air pollutants when compared to the control group. Conclusion Gas pollutants in the air can cause laryngeal mucosa injury in rats, and its mechanism may be related to Th1/Th2 imbalance, which is a Th2 dominant response.

Key words: Air pollution, Gaseous pollutants, Laryngeal mucosa, Cytokines, Acute injury

CLC Number: 

  • R767.8
[1] 范欣. 空气污染对喉部急性损伤及急性喉炎门诊量影响的研究[D]. 北京:北京大学,2017:29-37.
[2] 范欣, 丁文军, 王刚, 等. 细颗粒物及污染气体对大鼠喉黏膜的急性损伤[J]. 临床耳鼻咽喉头颈外科杂志, 2016, 30(23): 1876-1880. doi:10.13201/j.issn.1001-1781.2016.23.010. FAN Xin, DING Wenjun, WANG Gang, et al. PM2.5 and polluted gas exposure-induced acute injury in rats larynx membrane[J]. Journal of Clinical Otorhinolaryngology Head and Neck Surgery, 2016, 30(23): 1876-1880. doi:10.13201/j.issn.1001-1781.2016.23.010.
[3] 孟紫强. 环境毒理学[M].北京:中国环境科学出版社,2000:360-362.
[4] Lindgren A, Stroh E, Montnémery P, et al. Traffic-related air pollution associated with prevalence of asthma and COPD/chronic bronchitis. A cross-sectional study in Southern Sweden[J]. Int J Health Geogr, 2009, 8: 2. doi:10.1186/1476-072X-8-2.
[5] Takenoue Y, Kaneko T, Miyamae T, et al. Influence of outdoor NO2 exposure on asthma in childhood: meta-analysis[J]. Pediatr Int, 2012, 54(6): 762-769. doi:10.1111/j.1442-200X.2012.03674.x.
[6] Faustini A, Stafoggia M, Cappai G, et al. Short-term effects of air pollution in a cohort of patients with chronic obstructive pulmonary disease[J]. Epidemiology, 2012, 23(6): 861-879. doi:10.1097/EDE.0b013e31826767c2.
[7] Raaschou-Nielsen O, Bak H, Srensen M, et al. Air pollution from traffic and risk for lung cancer in three Danish cohorts[J]. Cancer Epidemiol Biomarkers Prev, 2010, 19(5): 1284-1291. doi:10.1158/1055-9965.EPI-10-0036.
[8] Chen F, Jackson H, Bina WF. Lung adenocarcinoma incidence rates and their relation to motor vehicle density[J]. Cancer Epidemiol Biomarkers Prev, 2009, 18(3): 760-764. doi:10.1158/1055-9965.EPI-08-0741.
[9] Hamra GB, Laden F, Cohen AJ, et al. Lung cancer and exposure to nitrogen dioxide and traffic: a systematic review and meta-analysis[J]. Environ Health Perspect, 2015, 123(11): 1107-1112. doi:10.1289/ehp.1408882.
[10] 曾强, 李国星, 张磊, 等. 中国六个城市大气中SO2和NO2对人群死亡的急性效应研究[J]. 中华预防医学杂志, 2015, 49(12): 1085-1091. doi:10.3760/cma.j.issn.0253-9624.2015.12.013. ZENG Qiang, LI Guoxing, ZHANG Lei, et al. Acute effects of SO2 and NO2 on mortality in the six cities of China[J]. Chinese Journal of Preventive Medicine, 2015, 49(12): 1085-1091. doi:10.3760/cma.j.issn.0253-9624.2015.12.013.
[11] 赵肃, 王任群, 金焕荣, 等. SO2和NO2混合气对大鼠呼吸系统的影响[J]. 中国公共卫生, 2004, 20(12): 1516-1517. doi:10.3321/j.issn:1001-0580.2004.12.037. ZHAO Su, WANG Renqun, JIN Huanrong, et al. Effect of mixtures of SO2 and NO2 on respiratory system in rats[J]. Chinese Journal of Public Health, 2004, 20(12): 1516-1517. doi:10.3321/j.issn:1001-0580.2004.12.037.
[12] 孟紫强, 桑楠, 张波. 二氧化硫体内衍生物诱发CHL细胞染色体畸变效应[J]. 中国环境科学, 2000, 20(1): 8-12. doi:10.3321/j.issn:1000-6923.2000.01.003. MENG Ziqiang, SANG Nan, ZHANG Bo. Induced effects of the in vivo derivatives of sulfur dioxide on chromosomal aberration in CHL cells[J]. China Environmental Science, 2000, 20(1): 8-12. doi:10.3321/j.issn:1000-6923.2000.01.003.
[13] 孟紫强, 桑楠, 张波, 等. 二氧化硫体内衍生物诱发小鼠骨髓嗜多染红细胞微核的效应[J]. 环境科学学报, 2000, 20(2): 239-243. doi:10.3321/j.issn:0253-2468.2000.02.022. MENG Ziqiang, SANG Nan, ZHANG Bo, et al. Micronuclei induced by SO2 derivatives in mouse bone marrow in vivo[J]. Acta Scientiae Circumstantiae, 2000, 20(2): 239-243. doi:10.3321/j.issn:0253-2468.2000.02.022.
[14] 孟紫强, 李瑞金, 白巨利, 等. 二氧化硫亚慢性染毒对大鼠肺细胞DNA的损伤[J]. 应用与环境生物学报, 2005, 11(6): 726-728. doi:10.3321/j.issn:1006-687X.2005.06.016. MENG Ziqiang, LI Ruijin, BAI Juli, et al. Damaging effect of SO2 on dna in lung cells of rats[J]. Chinese Journal of Applied & Environmental Biology, 2005, 11(6): 726-728. doi:10.3321/j.issn:1006-687X.2005.06.016.
[15] 孟紫强, 刘玉香, 武冬梅. 二氧化硫对小鼠肺及血清中细胞因子的影响[J]. 中国公共卫生, 2004, 20(9): 1050-1051. doi:10.3321/j.issn:1001-0580.2004.09.012. MENG Ziqiang, LIU Yuxiang, WU Dongmei. Effect of sulfur dioxide inhalation on cytokine levels in lungs and serum of mice[J]. Chinese Journal of Public Health, 2004, 20(9): 1050-1051. doi:10.3321/j.issn:1001-0580.2004.09.012.
[16] 肖纯凌, 王任群, 赵肃, 等. 大气污染物对大鼠肺炎性细胞因子影响[J]. 中国公共卫生, 2007, 23(1): 83-84. XIAO Chunling, WANG Renqun, ZHAO Su, et al. Effects of air pollutants on pulmonary inflammatory cytokines in rats[J]. Chinese Journal of Public Health, 2007, 23(1): 83-84.
[17] 王曦,吴人亮,郝天玲,等.IL-1β和TNF-α对培养的气道上皮细胞E钙黏着蛋白表达的影响 [J].同济医科大学学报,2000,29(3):189-191. WANG Xi, WU Renliang, HAO Tianling, et al. Effects of IL-1β and TNF-αon E-cadherin Expression of cultured airway epithelial cells[J]. Acta Univ Med Tongji, 2000,29(3):189-191.
[18] Birrell MA, Eltom S. The role of the NLRP3 inflammasome in the pathogenesis of airway disease[J]. Pharmacol Ther, 2011, 130(3): 364-370. doi:10.1016/j.pharmthera.2011.03.007.
[19] Nembrini C, Marsland BJ, Kopf M. IL-17-producing T cells in lung immunity and inflammation[J]. J Allergy Clin Immunol, 2009, 123(5): 986-994. doi:10.1016/j.jaci.2009.03.033.
[1] LI Lisha, GUAN Kai, WANG Zixi, CUI Le. The effects of air pollutants on the severity of allergic rhinitis symptoms caused by cypress pollen [J]. Journal of Otolaryngology and Ophthalmology of Shandong University, 2019, 33(1): 81-87.
[2] . The aqueous levels of TNF-α and IP-10 in different kinds of glaucoma and their correlation. [J]. JOURNAL OF SHANDONG UNIVERSITY (OTOLARYNGOLOGY AND OPHTHALMOLOGY), 2017, 31(1): 103-106.
[3] LIU Ye, SUN Dianshui, YIN Jinjun, XIA Ming, XU Ying, SUN Jianhua. Vestibular function damage mechanisms in nasopharyngeal carcinoma patients after intensity-modulated radiotherapy. [J]. JOURNAL OF SHANDONG UNIVERSITY (OTOLARYNGOLOGY AND OPHTHALMOLOGY), 2016, 30(6): 14-17.
[4] WANG Zengzhi. Immunosuppressive treatment for non-infectious uveitis and its effect on cytokines [J]. JOURNAL OF SHANDONG UNIVERSITY (OTOLARYNGOLOGY AND OPHTHALMOLOGY), 2015, 29(6): 64-67.
[5] ZHANG Qing-zhao1, SHIMADA Takashi2, CHENG Lei1. Effect of lysed Enterococcus faecalis FK-23 (LFK) on serum IL-12 in allergic rhinitis mice [J]. JOURNAL OF SHANDONG UNIVERSITY (OTOLARYNGOLOGY AND OPHTHALMOLOGY), 2014, 28(3): 7-9.
Full text



No Suggested Reading articles found!