Journal of Otolaryngology and Ophthalmology of Shandong University ›› 2020, Vol. 34 ›› Issue (5): 138-144.doi: 10.6040/j.issn.1673-3770.0.2020.247

Previous Articles     Next Articles

Effect of light-emitting diode light of different wavelengths on the rat lens: a preliminary study

  

  • Received:2020-05-29 Published:2020-11-17

PDF (PC)

137

Abstract: Objective This study was aimed at exploring the effects of light-emitting diode(LED)light of different peak wavelengths(622.791, 509.699, and 462.826 nm)on the rat lens, including damage to the morphology of the lens epithelial cells. Further, we tested the activities of the antioxidant enzymes superoxide dismutase(SOD)and glutathione peroxidase(GSH-Px)in the lens and compared the malondialdehyde(MDA)activity in the lens after irradiation by LED of different wavelengths. Methods Sprague Dawley rats were irradiated with LED light of different peak wavelengths(color temperature=4 500 K; illuminance: 500 lux)12 h a day for 5 days. Hematoxylin and eosin(HE)staining was used to observe the paraffin sections of rat lens epithelial cells. The activities of SOD, GSH-Px, and MDA in the lens were detected by the automatic biochemical analyzer. Results HE staining showed that lens epithelial cells in the control group were flat, monolayer, and uniform. In the blue-light group, lens epithelial cells were disordered, swollen, and changed from a single layer to double or even multiple layers. In the green-light group, lens epithelial cells were slightly swollen, and some areas showed a double-layer arrangement. In the red-light group, lens epithelial cells were flat, monolayer and uniform too. MDA activities in the rat lens of the control and red-, green-, and blue-light groups were approximately 0.004 3, 0.004 4, 0.015 6, and 0.017 8 U/mgprot, respectively. There were no significant differences between the red-light and control groups(P>0.05), but there were significant differences among the other groups(P<0.05). SOD activities in the lens of the control and red-, green-, and blue-light groups were 1.306 7, 7.926 7, 3.070 0, and 2.1233 U/mgprot, respectively, showing significant differences(P<0.05). GSH-Px activities in the lens of the control and red-, green-, and blue-light groups were 1.413 3, 9.793 3, 2.696 0, and 2.159 0 U/mgprot, respectively, showing significant differences(P<0.05). Conclusion The influence of LED light on rat lens epithelial cells is wavelength-dependent. Shorter wavelengths of LED light are associated with greater changes in the lens epithelial cell morphology, greater effects of SOD and GSH-Px activities in the lens, and more lipid peroxide accumulation in the lens.

Key words: LED light, Peak wavelength, Lens, Lens epithelial cells, Oxidative damage

CLC Number: 

  • R776.1
[1] Robman L, Taylor H. External factors in the development of cataract[J]. Eye(Lond), 2005, 19(10): 1074-1082. doi:10.1038/sj.eye.6701964.
[2] Zuclich JA, Lund DJ, Stuck BE. Wavelength dependence of ocular damage thresholds in the near-ir to far-ir transition region: proposed revisions to MPES[J]. Health Phys, 2007, 92(1): 15-23. doi:10.1097/01.HP.0000232188.69779.69.
[3] 赵星, 方志良, 母国光. LED投影光源的色度学特性研究[J]. 物理学报, 2007, 56(5): 2537-2540. doi:10.3321/j.issn:1000-3290.2007.05.011. ZHAO Xing, FANG Zhiliang, MU Guoguang. Study on the colorimetric properties of the LED projection sources[J]. Acta Physica Sinica, 2007, 56(5): 2537-2540. doi:10.3321/j.issn:1000-3290.2007.05.011.
[4] 马年骏. LED光源: 新一代绿色照明[J]. 上海节能, 2009(12): 19-23. MA Nianjun. LED light source: the new generation green illumination[J]. Shanghai Energy Conservation, 2009(12): 19-23.
[5] 麻佳琪, 张孟杨, 杨胡江, 等. 基于三基色 LED 的色匹配实验[J]. 物理实验, 2014, 34(4): 42-44, 48. doi:10.3969/j.issn.1005-4642.2014.04.011. MA Jiaqi, ZHANG Mengyang, YANG Hujiang, et al. Chromaticity experiments using tricolor LEDs[J]. Physics Experimentation, 2014, 34(4): 42-44, 48. doi:10.3969/j.issn.1005-4642.2014.04.011.
[6] 刘承东. LED路灯在道路照明中的应用[J]. 中国照明电器, 2009(3): 30-34. doi:10.3969/j.issn.1002-6150.2009.03.007. LIU Chengdong. Application of LED street lamp in road lighting[J]. China Light & Lighting, 2009(3): 30-34. doi:10.3969/j.issn.1002-6150.2009.03.007.
[7] 夏智锋. 基于LED光源的反光和背光器件的探索与设计[D]. 广州: 广东工业大学, 2016.
[8] 区志诚. LED灯具的现在与明天[J]. 经营管理者, 2010(5):361-361.
[9] 吴寿良. LED照明灯在现代建筑中的应用[J]. 智能建筑电气技术, 2014, 8(5): 23-25. doi:10.13857/j.cnki.cn11-5589/tu.2014.05.005. WU Shouliang. The application of LED in modern architecture[J]. Electrical Technology of Intelligent Buildings, 2014, 8(5): 23-25. doi:10.13857/j.cnki.cn11-5589/tu.2014.05.005.
[10] 周孟欣, 朱大庆, 廖欣怡. 低蓝光危害的白光LED光源的光谱设计与优化[J]. 激光与光电子学进展, 2019, 56(18): 182302. doi:10.3788/LOP56.182302. ZHOU Mengxin, ZHU Daqing, LIAO Xinyi. Spectral design and optimization of white LED light sources with low blue light hazard[J]. Laser & Optoelectronics Progress, 2019, 56(18): 182302. doi:10.3788/LOP56.182302.
[11] 肖芬. 紫外激发白光LED荧光粉的制备及发光特性研究[D]. 广州: 华南理工大学, 2011.
[12] 徐华. LED在学校照明中的应用探讨[J]. 智能建筑电气技术, 2018, 12(4): 17-19. doi:10.13857/j.cnki.cn11-5589/tu.2018.04.005. XU Hua. Discussion on the application of LED for school lighting[J]. Electrical Technology of Intelligent Buildings, 2018, 12(4): 17-19. doi:10.13857/j.cnki.cn11-5589/tu.2018.04.005.
[13] 姜翔, 林治. LED照明产品的蓝光危害检测和富蓝化探析[J]. 日用电器, 2018(2): 19-22. doi:10.3969/j.issn.1673-6079.2018.02.006. JIANG Xiang, LIN Zhi. Blue light hazard testing and rich blue environment analysis of LED luminaires[J]. Electrical Appliances, 2018(2): 19-22. doi:10.3969/j.issn.1673-6079.2018.02.006.
[14] 邓宗勇. 糖皮质激素性白内障发病机制的研究进展[J]. 医学综述, 2013, 19(17): 3155-3157. doi:10.3969/j.issn.1006-2084.2013.17.027. DENG Zongyong. Research advances in pathogenesis of glucocorticoid induced cataract[J]. Medical Recapitulate, 2013, 19(17): 3155-3157. doi:10.3969/j.issn.1006-2084.2013.17.027.
[15] 刘苹, 康刚劲. 细胞因子在后发性白内障发病机制中的研究进展[J]. 眼科新进展, 2013, 33(3): 297-300. doi:10.13389/j.cnki.rao.2013.03.024. LIU Ping, KANG Gangjin. Research progress of cytokines in mechanisms of posterior capsule opacification[J]. Recent Advances in Ophthalmology, 2013, 33(3): 297-300. doi:10.13389/j.cnki.rao.2013.03.024.
[16] Venugopalan P, Wang Y, Nguyen T, et al. Transplanted neurons integrate into adult retinas and respond to light[J]. Nat Commun, 2016, 7: 10472. doi:10.1038/ncomms10472.
[17] 袁牧, 王昌留, 王一斐, 等. 超氧化物歧化酶的研究进展[J]. 中国组织化学与细胞化学杂志, 2016, 25(6): 550-558. doi:10.16705/j.cnki.1004-1850.2016.06.015. YUAN Mu, WANG Changliu, WANG Yifei, et al. Progress in the research of superoxide dismutase[J]. Chinese Journal of Histochemistry and Cytochemistry, 2016, 25(6): 550-558. doi:10.16705/j.cnki.1004-1850.2016.06.015.
[18] 孙旋, 冯卓蕾, 陈娜. 川芎嗪对过氧化氢致大鼠视网膜神经节细胞氧化损伤的抑制作用及机制[J]. 眼科新进展, 2019, 39(6): 515-518. doi:10.13389/j.cnki.rao.2019.0118. SUN Xuan, FENG Zhuolei, CHEN Na. Inhibitory effect of ligustrazine on rat retinal ganglion cell oxidative damage induced by peroxide hydrogen and its mechanisms[J]. Recent Advances in Ophthalmology, 2019, 39(6): 515-518. doi:10.13389/j.cnki.rao.2019.0118.
[19] Cui Y, Xu N, Xu W, et al. Mesenchymal stem cells attenuate hydrogen peroxide-induced oxidative stress and enhance neuroprotective effects in retinal ganglion cells[J]. Vitro Cell Dev Biol - Animal, 2017, 53(4): 328-335. doi:10.1007/s11626-016-0115-0.
[20] 陈冉冉, 吴进忠, 吴子敬, 等. 不同力度屏蔽蓝光对正常成年人眼视功能的影响[J]. 中华眼视光学与视觉科学杂志, 2017, 19(11): 656-662. doi:10.3760/cma.j.issn.1674-845X.2017.11.004. CHEN Ranran, WU Jinzhong, WU Zijing, et al. Effect of different intensities of blue light shielding on visual function of normal adults[J]. Chinese Journal of Optometry Ophthalmology and Visual Science, 2017, 19(11): 656-662. doi:10.3760/cma.j.issn.1674-845X.2017.11.004.
[21] Zhu X, Guo X, Zhang JL, et al. Phosphor-free, color-mixed, and efficient illuminant: Multi-chip packaged LEDs for optimizing blue light hazard and non-visual biological effects[J]. Opt Lasers Eng, 2020, 134: 106174. doi:10.1016/j.optlaseng.2020.106174.
[22] Bian MJ, Du XY, Cui JG, et al. Celastrol protects mouse retinas from bright light-induced degeneration through inhibition of oxidative stress and inflammation[J]. J Neuroinflammation, 2016, 13: 50. doi:10.1186/s12974-016-0516-8.
[23] 王旻舒, 刘梦媛, 董栩然, 等. 年龄相关性白内障对老年人生物节律的影响[J]. 中华眼科杂志, 2016, 52(4): 309-314. doi:10.3760/cma.j.issn.0412-4081.2016.04.021. WANG Minshu, LIU Mengyuan, DONG Xuran, et al. Impact of age-related cataract on regulation of circadian rhythm in elderly[J]. Chinese Journal of Ophthalmology, 2016, 52(4): 309-314. doi:10.3760/cma.j.issn.0412-4081.2016.04.021.
[24] 丁辰, 蔡建明. 紫外线辐射对眼的损伤效应[J]. 中华放射医学与防护杂志, 2016, 36(2): 149-153. doi:10.3760/cma.j.issn.0254-5098.2016.02.014. DING Chen, CAI Jianming. Effect of ultraviolet rays and mechanism of injury to eyes[J]. Chinese Journal of Radiological Medicine and Protection, 2016, 36(2): 149-153. doi:10.3760/cma.j.issn.0254-5098.2016.02.014.
[25] 郑建. 应用于儿童的LED光源的光辐射安全性评价研究[D]. 杭州: 浙江大学, 2016. ZHENG Jian. Evaluation on photobiological safety of LED ligit sources for children[D]. Hangzhou: Zhejiang University, 2016.
[26] 汪雪梅. 老年白内障发病的氧化应激机制研究[J]. 实用临床医药杂志, 2015, 19(17): 196-197.
[27] 张硕, 刘立莉, 杨华, 等. 白光LED的光生物安全性[J]. 半导体技术, 2016, 41(5): 329-334, 370. doi:10.13290/j.cnki.bdtjs.2016.05.002. ZHANG Shuo, LIU Lili, YANG Hua, et al. Photobiological safety of white LEDs[J]. Semiconductor Technology, 2016, 41(5): 329-334, 370. doi:10.13290/j.cnki.bdtjs.2016.05.002.
[28] 施晓红, 陈超中. LED灯具蓝光危害评估方法[J]. 中国照明电器, 2013(3): 25-32. doi:10.3969/j.issn.1002-6150.2013.03.009. SHI Xiaohong, CHEN Chaozhong. Methods for evaluating LED luminaire blue light hazards[J]. China Light & Lighting, 2013(3): 25-32. doi:10.3969/j.issn.1002-6150.2013.03.009.
[29] 杨超普, 方文卿, 刘明宝, 等. 一种光源的蓝光危害与节律效应可视化评估方法[J]. 光谱学与光谱分析, 2018, 38(11): 3476-3482. doi:10.3964/j.issn.1000-0593(2018)11-3476-07. YANG Chaopu, FANG Wenqing, LIU Mingbao, et al. A visualization evaluation method for blue light hazard and circadian effect of light source[J]. Spectroscopy and Spectral Analysis, 2018, 38(11): 3476-3482. doi:10.3964/j.issn.1000-0593(2018)11-3476-07.
[1] HU Zunxia, SIMA Jing, QIN Bo, CAO Jiaguo, PAN Wei. Comparative study on the use of contact lens in Nd: YAG laser posterior capsulotomy [J]. Journal of Otolaryngology and Ophthalmology of Shandong University, 2022, 36(5): 41-45.
[2] DAI ChengOverview,LI BinzhongGuidance. Advances in multifocal soft corneal contact lens research [J]. Journal of Otolaryngology and Ophthalmology of Shandong University, 2022, 36(5): 100-105.
[3] LI Jing, ZHANG Hui, WANG Jing. A clinical study on the effect of using different intraocular lens implants on visual quality [J]. Journal of Otolaryngology and Ophthalmology of Shandong University, 2022, 36(2): 90-95.
[4] LI Bin, FANG Xuejun, WU De, HUANG Min. Agreement study of implantable collamer lens based on NK and KS formulas in the early stage after surgery [J]. Journal of Otolaryngology and Ophthalmology of Shandong University, 2021, 35(6): 33-41.
[5] ZHANG Ying, LEI Yulin, MA Zhixing, YANG Xinghua, ZHANG Jing, HOU Jie. Early clinical observation of corneal densitometry after SMILE combined with rapid corneal cross-linking [J]. Journal of Otolaryngology and Ophthalmology of Shandong University, 2021, 35(6): 52-58.
[6] XIAO Yu, ZHANG Youyin, DENG Yingping. Comparison of pain between two eye operations in patients undergoing implantable collamer lens implantation [J]. Journal of Otolaryngology and Ophthalmology of Shandong University, 2021, 35(4): 88-95.
[7] ZHOU Huaisheng, LIANG Wanling, LANG Haibo, XIAN Zhilin, YAN Shigang, Kong Xiangbin, SHEN Peiyang. Clinical application of the ultra-widefield laser ophthalmoscope to the preoperative examination of patients with rhegmatogenous retinal detachment [J]. Journal of Otolaryngology and Ophthalmology of Shandong University, 2021, 35(3): 96-99.
[8] WEI Peipei,LI Can. Visual quality and complications after implantation of multifocal intraocular lens [J]. Journal of Otolaryngology and Ophthalmology of Shandong University, 2021, 35(2): 141-146.
[9] LI Ying, JIANG Yang. A discussion on the choice of surgical methods for correction of myopia [J]. Journal of Otolaryngology and Ophthalmology of Shandong University, 2020, 34(2): 1-6.
[10] WAN Bo, LI Donghui, LUO Yan, LI Ying. Lens vault changes and associated factors after implantation of implantable collamer lenses with central flow [J]. Journal of Otolaryngology and Ophthalmology of Shandong University, 2020, 34(2): 36-41.
[11] WANG Xuanqi,CHEN XunReview,WANG XiaoyingGuidance. Research advances on vault and relevant factors after implantable collamer lens implantation [J]. Journal of Otolaryngology and Ophthalmology of Shandong University, 2020, 34(2): 72-78.
[12] YUE Shali, DENG Yingping. Implantable collamer lens implantation for regression of refraction after radical keratotomy: case report [J]. Journal of Otolaryngology and Ophthalmology of Shandong University, 2020, 34(2): 85-87.
[13] TAN Yan, LI Can. Progress in the research of intraocular lens power calculation formulas [J]. Journal of Otolaryngology and Ophthalmology of Shandong University, 2019, 33(6): 95-98.
[14] Danfeng ZHANG. Changes in the ocular biometric parameters before and after cycloplegia in juveniles with different degrees of myopia [J]. Journal of Otolaryngology and Ophthalmology of Shandong University, 2019, 33(5): 121-124.
[15] LIN Jia, LIAO Xuan, LAN Changjun, TAN Qingqing, WEN Baiwei, TIAN Jing. Visual quality research on a custom selection of aspheric intraocular lenses in cataract patients [J]. Journal of Otolaryngology and Ophthalmology of Shandong University, 2019, 33(4): 109-114.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
[1] . [J]. JOURNAL OF SHANDONG UNIVERSITY (OTOLARYNGOLOGY AND OPHTHALMOLOGY), 2006, 20(2): 116 -118 .
[2] ZHOU Zi-ning,JIN Guo-wei . [J]. JOURNAL OF SHANDONG UNIVERSITY (OTOLARYNGOLOGY AND OPHTHALMOLOGY), 2006, 20(5): 462 -465 .
[3] ZHOU Bin,LI Bin . Endoscopic sinus surgery for 75 patients with chronic sinusitis and nasal polyps[J]. JOURNAL OF SHANDONG UNIVERSITY (OTOLARYNGOLOGY AND OPHTHALMOLOGY), 2006, 20(1): 24 -26 .
[4] XU Sainan,YANG Lei . Apoptosis of epithelial cells in nasal polyps promoted by erythromycin[J]. JOURNAL OF SHANDONG UNIVERSITY (OTOLARYNGOLOGY AND OPHTHALMOLOGY), 2006, 20(1): 27 -29 .
[5] . [J]. JOURNAL OF SHANDONG UNIVERSITY (OTOLARYNGOLOGY AND OPHTHALMOLOGY), 2006, 20(1): 94 -95 .
[6] LIU Lian-he . Treatment of deep neck abscess in 37 cases[J]. JOURNAL OF SHANDONG UNIVERSITY (OTOLARYNGOLOGY AND OPHTHALMOLOGY), 2008, 22(2): 180 -181 .
[7] . [J]. JOURNAL OF SHANDONG UNIVERSITY (OTOLARYNGOLOGY AND OPHTHALMOLOGY), 2008, 22(3): 200 -203 .
[8] QIAO Yi,NI Guan-sen,CHEN Wen-wen . Effect of H-UPPP and nasal operations on obstructive sleep apnea syndrome in 38 cases
[J]. JOURNAL OF SHANDONG UNIVERSITY (OTOLARYNGOLOGY AND OPHTHALMOLOGY), 2008, 22(3): 206 -208 .
[9] WANG Xiao-feng,LIN Chang,CHENG Jin-mei . Expression of ABAD in inner ears and its clinical significance in different age mice[J]. JOURNAL OF SHANDONG UNIVERSITY (OTOLARYNGOLOGY AND OPHTHALMOLOGY), 2008, 22(3): 207 -211 .
[10] FAN Qi-jun,HUANG Zhi-wu,MEI Ling,XIAO Bo-kui . Expression of the heat shock protein 27 in rat cochlea induced by sodium salicylate injection by the FQ-PCR technique[J]. JOURNAL OF SHANDONG UNIVERSITY (OTOLARYNGOLOGY AND OPHTHALMOLOGY), 2008, 22(3): 212 -214 .
Website Copyright: Editorial Office of Journal of Otolaryngology and Ophthalmology of Shandong University
Address: No.27 Shanda South Road, Jinan, Shandong, China. Post code: 250100 Tel: +86-0531-88366912 Email: ebhxb@sdu.edu.cn Supported by Beijing Magtech Co.Ltd