Journal of Otolaryngology and Ophthalmology of Shandong University ›› 2023, Vol. 37 ›› Issue (5): 6-15.doi: 10.6040/j.issn.1673-3770.0.2022.246

• Original Article • Previous Articles     Next Articles

Bioinformatic analysis of the transcriptome of basal stem cells of individuals with chronic rhinosinusitis with nasal polyps

WANG Mingming, LUO Yang, HE Shaojuan, ZHANG Xianxing, LI Xuezhong   

  1. Department of Otorhinolaryngology, Qilu Hospital of Shandong University, Jinan 250012, Shandong, China
  • Published:2023-10-13

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Abstract: Objective The differentially expressed genes(DEGs)involved in the proliferation and differentiation of basal stem cells(BSCs)of individuals with chronic rhinosinusitis with nasal polyps(CRSwNP)were analyzed using bioinformatics to identify the mechanisms underlying the epithelial barrier damage in CRSwNP and to gain insights for potential treatment strategies. Methods The transcriptome chip microarray dataset of the proliferation and differentiation of BSCs of nasal polyps was downloaded from the gene expression omnibus(GEO)database. R software was then used for weighted gene co-expression network analysis(WGCNA)to build networks and screen disease-associated genes on the basis of their differential expression between the CRSwNP group and the normal control group, and the online tool DAVID was used to perform gene ontology(GO)analysis and Kyoto Encyclopedia of Genes and Genomes(KEGG)pathway enrichment of the DEGs. A protein interaction network of the DEGs was constructed using the STRING database and analyzed by applying the MCODE plugin in Cytoscape software. Finally, a transcription factor network of core genes was constructed using the NetworkAnalyst tool and by integrating the transcription factor database. Results This study found that a total of 175 disease-associated genes showing differential expression between the BSC of the CRSwNP and normal control groups(P<0.05, ∣logFC∣>1). GO and KEGG pathway analysis revealed that these DEGs were mainly enriched in the negative regulation of endopeptidase activity, serine-type endopeptidase inhibitor activity, and the Wnt signaling pathway. Analysis of the constructed protein interaction network was performed to identify core genes such as IVL and SPRR2A. These genes were all upregulated during the differentiation of BSCs, and they were found to interact with each other. Furthermore, this analysis revealed that the transcription factor GATA2 plays a critical role in the differentiation of BSCs. Conclusion IVL, SPRR2A, and GATA2 may be involved in the mechanism through which the epithelial barrier is damaged in CRSwNP. Inhibition of endopeptidase activity may be the key mechanism involved in the epithelial barrier damage. This study provides new insights into the biological mechanism underlying the epithelial barrier damage in CRSwNP and our findings could help develop a treatment strategy for CRSwNP.

Key words: Chronic sinusitis, Epithelial barrier, Basal stem cells, Bioinformatics

CLC Number: 

  • R765.4+1
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