单细胞测序分析小鼠耳蜗螺旋神经节与内外毛细胞间的细胞信号通路

doi:10.6040/j.issn.1673-3770.0.2023.294

Abstract

Abstract: Objective To investigate the cell populations of cochlea during hearing formation and maturation phases and the ligand receptors that regulating the expression of signaling pathways between spiral ganglion neuron and inner and outer hair cells. Methods We investigated the potential cell-cell interactions between inner and outer hair cells and adjacent cells by collecting single-cell sequencing data from mouse cochlea and using CellChat software. Results Seurat analysis showed 15 significant groups of cell types in the mouse cochlea, including Fibroblast, Hensen's cell, Tympanic Border Cell, Coch+/Spp1+ Fibroblast, Inner/Outer Sulcus Cell, Inner Phalangeal Cell/Inner Border Cell, Outer Hair Cell, Reissner's Membrane, Macrophage, Spindle Cell/Root Cell, Spiral Ganglion Neuron, Intermediate Cell, Schwann Cell/Satellite Glial Cell, Inner Hair Cell and Red Blood Cell. Through the CellChat analysis, we observed that the communication relationship between inner and outer hair cells and spiral ganglion neurons in the P14 mouse cochlea was mainly mediated by the Bdnf/Ntrk2 signaling pathway, and Gdf6/(Bmpr1a+Bmpr2), Bmp6/(Bmpr1a+Bmpr2), Ntf3/Ntrk3, Ntf3/Ntrk2, Spp1/Cd44, Spp1/(Itgav+Itgb1), Pdgfa/Pdgfra, Gas6/Tyro3 signaling pathways during P28 period. Conclusion This study initially identified specific ligand receptors between spiral ganglion neuron and inner and outer hair cells during the cochlear hearing formation and cochlea maturation phases based on single-cell transcriptome data.

Key words: Single-cell RNA sequencing, Spiral ganglion neurons, Hair cells, Intercellular communication networks

CLC Number:

R764

DONG Wenqi, YU Dongzhen. Single-cell RNA sequencing reveals cellular communication between spiral ganglion neurons and inner and outer hair cells in postnatal mice[J].Journal of Otolaryngology and Ophthalmology of Shandong University, 2025, 39(3): 51-60.

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Single-cell RNA sequencing reveals cellular communication between spiral ganglion neurons and inner and outer hair cells in postnatal mice

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