Journal of Otolaryngology and Ophthalmology of Shandong University ›› 2024, Vol. 38 ›› Issue (4): 22-27.doi: 10.6040/j.issn.1673-3770.0.2023.200

• Original Article • Previous Articles    

Clomipramine hydrochloride protects auditory hair cells from neomycin-induced damage

ZHOU Jing1, BI Xiuli2, XIAO Yu3, HU Jun4, FU Xiaolong2, YU Yafeng1   

  1. 1. Department of Otorhinolaryngology & Head and Neck Surgery, The First Affiliated Hospital of Soochow University, Suzhou 215006, Jiangsu, China2. Science and Technology Innovation Center, Shandong First Medical University, Jinan 250117, Shangdong, China3. College of Life Sciences, Shandong University, Jinan 250100, Shangdong, China4. Department of Otorhinolaryngology & Head and Neck Surgery, Wenzhou Medical University, Wenzhou 325035, Zhejiang, China
  • Published:2024-07-09

PDF (PC)

100

Abstract: Objective The aim of this study was to identify small-molecule drugs that inhibit neomycin-induced hair-cell-like cell(HEI-OC1)death, and to verify their effects on hair cells in cochlear explants and the lateral line of zebrafish. Methods Small-molecule drugs that increased the viability of HEI-OC1 cells were identified using Cell Counting Kit-8. The protective effect of the identified small-molecule drugs was verified in hair cells of cochlear explants and zebrafish by Immunofluorescence staining. Results Of the 350 small-molecule drugs screened, clomipramine hydrochloride was found to significantly improve the survival rate of neomycin-treated HEI-OC1 cells. In addition, clomipramine hydrochloride was found to have a protective effect on hair cells of neomycin-treated mouse cochlear explants and the lateral line of zebrafish. Conclusion Clomipramine hydrochloride, which as an FDA-approved clinical drug, has a protective effect against neomycin-induced hair cell damage.

Key words: Neomycin, Aminoglycosides, Hair cells, Clomipramine hydrochloride, Deafness

CLC Number: 

  • R764.43
[1] Prasad KN, Bondy SC. Increased oxidative stress, inflammation, and glutamate: potential preventive and therapeutic targets for hearing disorders[J]. Mech Ageing Dev, 2020, 185: 111191. doi:10.1016/j.mad.2019.111191
[2] 丘春柳. 舍曲林对强迫症患者临床症状、执行功能和职业功能的远期效果 [J]. 中华行为医学与脑科学杂志,2014,23(6): 507-510. doi: 10.3760/cma.j.issn.1674-6554.2014.06.009
[3] He ZH, Sun S, Waqas M, et al. Reduced TRMU expression increases the sensitivity of hair-cell-like HEI-OC-1 cells to neomycin damage in vitro[J]. Sci Rep, 2016, 6: 29621. doi:10.1038/srep29621
[4] Zhou MJ, Sun GY, Zhang LL, et al. STK33 alleviates gentamicin-induced ototoxicity in cochlear hair cells and House Ear Institute-Organ of Corti 1 cells[J]. J Cell Mol Med, 2018, 22(11): 5286-5299. doi:10.1111/jcmm.13792
[5] Kalinec GM, Webster P, Lim DJ, et al. A cochlear cell line as an in vitro system for drug ototoxicity screening[J]. Audiol Neurotol, 2003, 8(4): 177-189. doi:10.1159/000071059
[6] He ZH, Sun S, Waqas M, et al. Reduced TRMU expression increases the sensitivity of hair-cell-like HEI-OC-1 cells to neomycin damage in vitro[J]. Sci Rep, 2016, 6: 29621. doi:10.1038/srep29621
[7] Kalinec G, Thein P, Park C, et al. HEI-OC1 cells as a model for investigating drug cytotoxicity[J]. Hear Res, 2016, 335: 105-117. doi:10.1016/j.heares.2016.02.019
[8] Yang QQ, Zhou YW, Yin HY, et al. PINK1 protects against gentamicin-induced sensory hair cell damage: possible relation to induction of autophagy and inhibition of p53 signal pathway[J]. Front Mol Neurosci, 2018, 11: 403. doi:10.3389/fnmol.2018.00403
[9] Zhou MJ, Sun GY, Zhang LL, et al. STK33 alleviates gentamicin-induced ototoxicity in cochlear hair cells and House Ear Institute-Organ of Corti 1 cells[J]. J Cell Mol Med, 2018, 22(11): 5286-5299. doi:10.1111/jcmm.13792
[10] Ogier JM, Burt RA, Drury HR, et al. Organotypic culture of neonatal murine inner ear explants[J]. Front Cell Neurosci, 2019, 13: 170. doi:10.3389/fncel.2019.00170
[11] Nadol JB Jr. Hearing loss[J]. N Engl J Med, 1993, 329(15): 1092-1102. doi:10.1056/nejm199310073291507
[12] Forge A, Schacht J. Aminoglycoside antibiotics[J]. Audiol Neurotol, 2000, 5(1): 3-22. doi:10.1159/000013861
[13] Yumi Sugimoto, et al. The tricyclic antidepressant Clomipramine increases plasma glucose levels of mice[J]. Pharmacol Sci, 2003,93(1):74-79.doi: 10.1254/jphs.93.74
[14] Berton O, Nestler EJ. New approaches to antidepressant drug discovery: beyond monoamines[J]. Nat Rev Neurosci, 2006, 7(2): 137-151. doi:10.1038/nrn1846
[15] Mushtaq Ahmed. Comparative study of the inhibitory effect of antidepressants on cholinesterase activity in Bungarus sindanus(krait)venom, human serum and rat striatum[J]. Enzyme Inhib Med Chem, 2008,23(6):912-7.doi: 10.1080/14756360701809977
[16] Cavaliere F, Fornarelli A, Bertan F, et al. The tricyclic antidepressant clomipramine inhibits neuronal autophagic flux[J]. Sci Rep, 2019, 9(1): 4881. doi:10.1038/s41598-019-40887-x
[17] Yao Q, DeSmidt AA, Tekin M, et al. Hearing assessment in zebrafish during the first week postfertilization[J]. Zebrafish, 2016, 13(2): 79-86. doi:10.1089/zeb.2
[18] Riley BB, Moorman SJ. Development of utricular otoliths, but not saccular otoliths, is necessary for vestibular function and survival in zebrafish[J]. J Neurobiol, 2000, 43(4): 329-337. doi:10.1002/1097-4695(20000615)43: 4<329: aid-neu2>3.0.co;2-h
[19] Sun P, Zhang YL, Zhao F, et al. An assay for systematically quantifying the vestibulo-ocular reflex to assess vestibular function in zebrafish larvae[J]. Front Cell Neurosci, 2018, 12: 257. doi:10.3389/fncel.2018.00257
[20] Tanya T Whitfield, Bruce B Riley, et al. Development of the zebrafish inner ear[J]. Dev Dyn, 2002,223(4):427-458.doi: 10.1002/dvdy.10073
[21] Ghysen A, Dambly-Chaudière C. The lateral line microcosmos[J]. Genes Dev, 2007, 21(17): 2118-2130. doi:10.1101/gad.1568407
[22] Zhong ZH, Fu XL, Li H, et al. Citicoline protects auditory hair cells against neomycin-induced damage[J]. Front Cell Dev Biol, 2020, 8: 712. doi:10.3389/fcell.2020.00712
[23] Zheng ZW, Tang DM, Zhao LP, et al. Erratum to “liproxstatin-1 protects hair cell-like HEI-OC1 cells and cochlear hair cells against neomycin ototoxicity”[J]. Oxid Med Cell Longev, 2021: 9873282. doi:10.1155/2021/9873282
[24] Miyoshi H, Otomo M, Takahashi K. Clomipramine inhibits dynamin GTPase activity by L-α-phosphatidyl-L-serine stimulation[J]. J Biochem, 2023, 174(3): 267-272. doi:10.1093/jb/mvad038
[1] ZHANG Jiajia, SHI Anni, XING Yu, HONG Yuying, BAI Peng. Acupuncture combined with western medicine for the treatment of total deafness in sudden sensorineural hearing loss: a case report and literature review [J]. Journal of Otolaryngology and Ophthalmology of Shandong University, 2024, 38(3): 88-92.
[2] LI Wei, ZHAO Yi, GE Yueming, FU Hongtao, WANG Jindong, ZHANG Xiaolong, DONG Jie, CHENG Yuxiang. Hotspots of deafness gene mutation and its effect on hearing in newborns [J]. Journal of Otolaryngology and Ophthalmology of Shandong University, 2024, 38(1): 1-8.
[3] XU Haiyan, CAO Wei, FAN Dachuan. Efficacy of compound sound therapy for patients with tinnitus and sudden deafness [J]. Journal of Otolaryngology and Ophthalmology of Shandong University, 2023, 37(4): 38-45.
[4] SUN Yongqiang, SONG Zhongyi, WANG Chunfang, LI Xiao, FU Ying, PAN Xinliang. Prognosis and influencing factors in sudden deafness patients with acoustic stapedius reflex threshold [J]. Journal of Otolaryngology and Ophthalmology of Shandong University, 2023, 37(2): 7-14.
[5] YANG Kun, CHEN Lijuan, HE Xiaodan, LIU Zhiqi, SHA Suhua. Comparative study of ototoxicity between kanamycin and 2-hydroxypropyl-β-cyclodextrin [J]. Journal of Otolaryngology and Ophthalmology of Shandong University, 2022, 36(4): 6-11.
[6] ZHANG Yanhong, LI Juanjuan, ZENG Xianhai, GOU Lingshan, WANG Zhaoxia, WEI Jianfang, MA Fang, QIU Shuqi. Clinical application of target gene panel testing in genetic diagnosis of deafness [J]. Journal of Otolaryngology and Ophthalmology of Shandong University, 2022, 36(4): 27-34.
[7] SHI Anni, ZHANG Jiajia, BAI Peng, ZHANG Chongyang. Analysis of a therapeutic modality for sudden deafness utilizing neck acupuncture comprising seven lines of treatment [J]. Journal of Otolaryngology and Ophthalmology of Shandong University, 2022, 36(4): 103-107.
[8] LI Zhen, CUI Limei,SUN Yan. Advances in the role of bone morphogenetic protein-4 in inner ear development and regeneration of hair and spiral ganglion cells [J]. Journal of Otolaryngology and Ophthalmology of Shandong University, 2022, 36(2): 108-112.
[9] LIN Yushan, LU Biaoqing. Treatment of sudden seafness by heart and spleen [J]. Journal of Otolaryngology and Ophthalmology of Shandong University, 2020, 34(6): 6-11.
[10] Yiliang YE,Biaoqing LU. Therapeutic outcome analysis of 704 cases of sudden deafness [J]. Journal of Otolaryngology and Ophthalmology of Shandong University, 2019, 33(5): 44-47.
[11] Xin CAO,Haijin YI,Xin LI,Jia XU,Juanjuan GAO. Secretory otitis media with sudden hearing loss: three cases′ report and a literature review [J]. Journal of Otolaryngology and Ophthalmology of Shandong University, 2019, 33(5): 56-61.
[12] Xiaosheng ZHONG,Haidi YANG,Yiqing ZHENG. Treatment and outcome of tinnitus in sudden deafness [J]. Journal of Otolaryngology and Ophthalmology of Shandong University, 2019, 33(4): 43-46.
[13] ZHAO Junying, WANG Jianming. Correlation between unilateral sudden deafness and imaging features of vertebrobasilar artery [J]. Journal of Otolaryngology and Ophthalmology of Shandong University, 2019, 33(4): 47-51.
[14] ZHANG Junjun, LIU Haili, LIU Jiafu, QIN Xiaofeng, DU Hongming, WANG Houhui. Clinical efficacy and safety of different methods of administration of glucocorticoids in the treatment of sudden deafness [J]. Journal of Otolaryngology and Ophthalmology of Shandong University, 2019, 33(4): 52-55.
[15] TONG Qiaozhen, ZHAO Yan, CHENG Sihua. The constructive and mediating effects of knowledge/attitude/belief/practice interference on negative emotion in patients with sudden deafness [J]. Journal of Otolaryngology and Ophthalmology of Shandong University, 2019, 33(4): 162-164.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
No Suggested Reading articles found!
Website Copyright: Editorial Office of Journal of Otolaryngology and Ophthalmology of Shandong University
Address: No.27 Shanda South Road, Jinan, Shandong, China. Post code: 250100 Tel: +86-0531-88366912 Email: ebhxb@sdu.edu.cn Supported by Beijing Magtech Co.Ltd