Journal of Otolaryngology and Ophthalmology of Shandong University ›› 2024, Vol. 38 ›› Issue (4): 22-27.doi: 10.6040/j.issn.1673-3770.0.2023.200

• Original Article • Previous Articles    

Clomipramine hydrochloride protects auditory hair cells from neomycin-induced damage

ZHOU Jing1, BI Xiuli2, XIAO Yu3, HU Jun4, FU Xiaolong2, YU Yafeng1   

  1. 1. Department of Otorhinolaryngology & Head and Neck Surgery, The First Affiliated Hospital of Soochow University, Suzhou 215006, Jiangsu, China2. Science and Technology Innovation Center, Shandong First Medical University, Jinan 250117, Shangdong, China3. College of Life Sciences, Shandong University, Jinan 250100, Shangdong, China4. Department of Otorhinolaryngology & Head and Neck Surgery, Wenzhou Medical University, Wenzhou 325035, Zhejiang, China
  • Published:2024-07-09

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Abstract: Objective The aim of this study was to identify small-molecule drugs that inhibit neomycin-induced hair-cell-like cell(HEI-OC1)death, and to verify their effects on hair cells in cochlear explants and the lateral line of zebrafish. Methods Small-molecule drugs that increased the viability of HEI-OC1 cells were identified using Cell Counting Kit-8. The protective effect of the identified small-molecule drugs was verified in hair cells of cochlear explants and zebrafish by Immunofluorescence staining. Results Of the 350 small-molecule drugs screened, clomipramine hydrochloride was found to significantly improve the survival rate of neomycin-treated HEI-OC1 cells. In addition, clomipramine hydrochloride was found to have a protective effect on hair cells of neomycin-treated mouse cochlear explants and the lateral line of zebrafish. Conclusion Clomipramine hydrochloride, which as an FDA-approved clinical drug, has a protective effect against neomycin-induced hair cell damage.

Key words: Neomycin, Aminoglycosides, Hair cells, Clomipramine hydrochloride, Deafness

CLC Number: 

  • R764.43
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